| Project/Area Number |
09640779
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | Himeji Institute of Technology |
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Principal Investigator |
SONOBE Seiji Himeji Institute of Technology, Assistant Professor, 理学部, 助教授 (30197024)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Plant cell / Cytokinesis / Cell Plate / Golgi apparatus / Xyloglucan / tobacco BY-2 / 隔膜形成体 / ゴルジ装置 / タバコ培養細胞BY-2 |
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| Research Abstract |
Isolation of golgi-derived vesicles participating in cell plate formation in higher plant cells was carried out using tobacco BY-2 cells whose cell-cycle had been synchronized. Protoplasts prepared from cells at anaphase were homogenized and fractionated by centrifugation in density gradient to obtain vesicle fractions. To identify golgi-derived vesicles, antibody against xyloglucan was used. Although XG-containing vesicles were distributed to a broad range of densities, vesicles which had an affinity to microtubules were found in a fraction. On the other hand, new informations on accumulation and fusion of golgi-derived vesicles were obtained. When BY-2 cells were cultured in the presence of propyzamide for a long time (8-10 hr), nuclear envelope was reappeared and simultaneously, a cell-plate like structure was formed close to the nucleus. Staining the structure with aniline blue indicated synthesis of callose. Electron microscopy revealed formation of a cell-plate with an irregular shape. Caffein or monen sin inhibited its formation. These results indicated that golgi-derived vesicles had accumulated aroun the nucleus by metaphase and fusion of them was induced when the cell-cycle had been shifted from mitotic phase to interphase. Therefore, cells at metaphase are thought to the best material for preparation of golgi-derived vesicles.
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