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Physiological roles of insulin-like growth factor I in the mouse uterine endometrium.

Research Project

Project/Area Number 09640791
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物形態・構造
Research InstitutionOkayama University

Principal Investigator

TAKAHASHI Sumio  Okayama University, Faculty of Science, Professor, 理学部, 教授 (90144807)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsMouse / uterus / endometrium / insulin-like growth factor I / IGF-I / proliferation / 増殖
Research Abstract

Estrogens and progestin regulate the growth of uterine endometrial cells. Estrogen-induced proliferation and differentiation of mouse endometrial cells may be mediated by growth factors. This study was undertaken to determine whether insulin-like growth factor (IGF-I)' stimulates proliferation of mouse epithelial and stromal cells in primary serum-free culture. In both epithelial and stromal cells, IGF-I mRNA and IGF-I receptor (type r) mRNA were detected. Estrogen stimulated IGF-I synthesis, and combination of estrogen and progesterone stimulated IGF-I receptor synthesis at the level of gene transcription. IGF-I stimulated the proliferation of both the epithelial and stromal cells in a dose-dependent manner. These study altogether indicate that endometrial IGF-I regulates the growth of endometrial cells in a paracrine or autocnine manner, highly probably as mediator of estrogen actions.
We successfully reconstituted the uterine endometrium in vitro. The stromal cells were suspended in collagen gel, and the epithelial cells were plated on the stromal cell layer in the collagen gel. Junctional complex structure (tight-junction and zonula adherence) and microvilli were observed in the epithelial cells. Co-culture of epithelial cells and stromal cells increased the number of epithelial cells cultured. This suggests that the stromal cells affect the growth of epithelial cells. Molecular mechanism of this interaction between the epithelial cells and stromal cells needs to be clarified.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Shiraga, M.: "Insulin like growth factro I stimulates proliferation of mouse uterine epithelial cells in primary culture." Proc.Soc.Exp.Biol.Med.215. 412-417 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Oomizu, S.: "Stimulatory effects of insulin like growth factor I on proliferation of mouse pituitary cells in serum-free culture." J.Endocrinol.157. 53-62 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takeuchi, S.,: "Molecular cloning and sequence analysis of the chicken melanocortin 2 (ACTH) receptor gene." Biochim.Biophys.Acta. 1403. 102-108 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Morooka, Y.: "Augmentation of prolactin release by α melanocyte stimulating hormone is possibly mediated by melanocortin 3-receptors in the mouse anterior pituitary cells." Zool.Sci.15. 567-572 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Honda, J.: "Insulin-like growth factor-I and its receptor in mouse pituitary glands." Zool.Sci.15. 573-579 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yayoi, Y.: "Expression of neural cell adhesion molecules in the mouse anterior pituitary gland and pituitary tumor cells." Biomed.Res.19. 269-277 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takeuchi, S.: "Melanocortin receptor genes in the chicken-tissue distributions." Gen.Comp.Endocrinol.112. 220-231 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takeuchi, S.: "A possible involvement of melanocortin 3 receptor in the regulation of adrenal gland function in the chicken." Biochim.Biophys.Acta. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shiraga, M., Takahashi, S., Miyake, T., Takeuchi, S.and Fukamachi, H.: "Insulin-like growth factor-I stimulates proliferation of mouse uterine epithelial cells in primary culture." Proc.Soc.Exp.Biol.Med.215. 412-417 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Oomizu, S., Takeuchi, S.and Takahashi, S.: "Stimulatory effects of insulin-like growth factor I on proliferation of mouse pituitary cells in serum-free culture." J.Endocrinol.157. 53-62 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takeuchi, S., Kudo, T.and Takahashi, S.: "Molecular cloning and sequenceanalysis of the chicken melanocortin 2 (ACTH) -receptor gene." Biochim.Biophys.Acta. 1403. 102-108 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Morooka, Y., Oomizu, S., Takeuchi, S., Takahashi, S.: "Augmentation of prolactin release by alpha-melanocyte stimulating hormone is possibly mediated by melanocortin 3-receptors in the mouse anterior pituitary cells." Zool.Sci.15. 567-572 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Honda, J., Takeuchi, S., Fukamachi, H.and Takahashi, S.: "Insulin-like growth factor-I and its receptor in mouse pituitary glands." Zool.Sci.15. 573-579 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yayoi, Y., Takahashi, S., Takeuchi, S., Takeuchi, M., Seki, T.and Arai, Y.: "Expression of neural cell adhesion molecules in the mouse anterior pituitary gland and pituitary tumor cells." Biomed.Res.19. 269-277 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takeuchi, S.and Takahashi, S.: "Melanocortin receptor genes in the chicken tissue distributions." Gen.Comp.Endocrinol.112. 220-231 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takeuchi, S., and Takahashi, S.: "A possible in volvement of melanocortin 3-receptor in the regulation of adrenal gland function in the chicken." Biochim.Biophys.Acta. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Oomizu,S.: "Stimulatory effects of insulin like growth factor I on proliferation of mouse pituitary cells in serum-free culture." J.Endocrinol.157. 53-62 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takeuchi,S.,: "Molecular cloning and sequence analysis of the chicken melanocortin 2 (ACTH) receptor gene." Biochim.Biophys.Acta. 1403. 102-108 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Morooka,Y.: "Augmentation of prolactin release by α melanocyte stimulating hormone is possibly mediated by melanocortin 3-receptors in the mouse anterior pituitary cells." Zool.Sci.15. 567-572 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Honda,J.: "Insulin-like growth factor-I and its receptor in mouse pituitary glands." Zool.Sci.15. 573-579 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yayoi,Y.: "Expression of neural cell adhesion molecules in the mouse anterior pituitary gland and pituitary tumor cells." Biomed.Res.19. 269-277 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takeuchi,S.: "Melanocortin receptor genes in the chicken-tissue distributions." Gen.Comp.Endocrinol.112. 220-231 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takeuchi,S.: "A possible involvement of melanocortin 3 receptor in the regulation of adrenal gland function in the chicken." Biochim.Biophys.Acta. (印刷中).

    • Related Report
      1998 Annual Research Report
  • [Publications] Shiraga,Masahiro: "Insulin-like growth factor・1 stimulates proliferation of mouse uterine epithelial cells in primary culture" Proceedings of the Society for Experimental Biology and Medicine. 215・4. 412-417 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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