| Project/Area Number |
09640806
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
動物生理・代謝
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| Research Institution | Osaka University |
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Principal Investigator |
OZAKI Koichi Graduate School of Science Research Associate, 大学院・理学研究科, 助手 (90194539)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | vesicle transport / Rab / photoreceptor cell / neuron / rhodopsin / endocytosis / Drosophila / Rab蛋白質 / ゴルジ体 / 小胞体 |
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| Research Abstract |
In this project, the network system of vesicle transport and the communication between its elementary steps have been studied with special attention to Rab proteins by using Drosophila photoreceptor cell as a model cell for neuron. For this purpose, I investigated the tissue- and intracellular-distribution of Rab proteins, and carried out the biochemical and morphological analyses of the phenotypes of the dominant negative mutants of these Rab proteins. The results are as follows :
1. Proteins localized in the photoreceptive membrane domain as well as those in the axonal domain are transported from the rER to the Golgi body under the control of RAB1.
2. RAB2 would possibly control all pathways from the Golgi body to the photo-receptive membrane, the axonal membrane and the lysosome. This suggests that RAB2 would links between these pathways.
3. RAB11 specifically controls the vesicle transport between the trans-Golgi network and the photoreceptive membrane. Moreover, PAB11 may possibly inform the condition of the photoreceptive membrane because the morphological phenotype of photoreceptor cells in this mutant closely resembles to that of the photoreceptor cells abnormally activated by light.
4. The early step of endocytosis in the photoreceptor membrane region is controlled by RAB5. In contrast, the recycling of the synaptic vesicles, a major endocytic system in the axonal region, is independent of RAB5. This suggests that the axonal region would develop the partly self-supported system in the photoreceptor cell.
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