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Investigation on diversity in the structure of cellulose-synthesizing terminal complexes among algal groups possessing chlorophyll a and c.

Research Project

Project/Area Number 09640828
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 系統・分類
Research InstitutionKochi University

Principal Investigator

OKUDA Kazuo  Kochi University, Faculty of Science, Professor, 理学部, 教授 (40152417)

Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsChlorophyll a and c / Cellulose microfibril / Cellulose-synthesizing terminal complex / diversity / Freeze fracture electron microscopy / クロロフィルa+c植物 / セルロース / 細胞外被 / 凍結割断法
Research Abstract

The structure of cellulose microfibrils (CMFs) and cellulose-synthesizing terminal complexes (TCs) in some species with chlorophyll a and c such as phaeophycean, xanthophycean and dinophycean algae was examined by freeze fracture electron microscopy. Both of Sphacelaria rigidula (Phaeophyceae) and Botridium sp. (Xanthophyceae) synthesized ribbon-like CMFs. However, Sphacelaria had TCs whose subunits are arranged in a single linear row, whereas TCs in Botridium consisted of several diagonal rows of subunits. It was found first that alginates are present in Xanthophyceae besides in Phaeophyceae, indicating a phylogenetically close relationship between Xanthophyceae and Phaeophyceae. These results suggest that the TCs in both or either of - Xanthophyceae and/or Phaeophyceae had been acquired at divergence of these two clades. It was evident in the dinophycean Scrippsiella hexapraecingra that the thecal plates contain CMFs which form bundles consisting of several fine components by electron diffraction methods. A new TC was found in this alga.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] 奥田一雄、峯一郎: "藻類セルロースの生合成"Cellulose communications. 4. 101-107 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Okuda K.et al.: "Evidence for laternal association of nascent cellulose sheets synthesized by cellulose-synthesized by cellulose-sunthesizing teminal complexes"Memoirs of the Faculty of Science,Kochi Univ.,Ser.D(Biol.). 19. 1-5 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 奥田一雄: "藻類のセルロース合成"電子顕微鏡. 34. 81-86 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chi E-S.et al.: "Immunolgold-labeling analysis of alginate distributions in the cell walls of chromophyte algae"Phycological Research. 47. 53-60 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sekida S.et al.: "An improved method for fixing dinoflagellate non-motile cells for electron microscopy"Memoirs of the Faculty of Science,Kochi Univ.,Ser.D(Biol.). 20. 11-15 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sekida S.et al.: "Direct evidence for cellulose microfibrils present in thecal plates of the dinoflagellate Scrippsiella Hexapraecingula"Hikobia. 13. 65-69 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Okuda K & Mine l: "Biosynthesis of algal cellulose"Cellulose communications. 4. 101-107 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Okuda K et al.: "Evidence for lateral association of nascent cellulose sheets synthesized by cellulose-synthesized by cellulose-synthesizing complexes."Memoirs of the Faculty of Science, Kochi Univ., Ser. D (Biol.). 19. 1-5 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Okuda K: "Cellulose synthesis in algae"Electron Microscopy. 34. 81-86 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chi E-S et al.: "Immunogold-labeling analysis of alginate distributions in the cell walls of chromophyte algae."Phycological Research. 47. 53-60 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sekida S et al.: "An improved method for fixing dinoflagellate non-motile cells for electron microscopy."Memoirs of the Faculty of Science, Kochi Univ., Ser. D (Biol.). 20. 11-15 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sekida S et al.: "Direct evidence for cel-lulose microfibrils present in thecal plates of the dinoflagellate Scrippsiella hexapraecingula."Hikobia. 13. 65-69 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 奥田一雄、峯一朗: "藻類セルロースの生合成"Cellulose communications. 4. 101-107 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] Okuda K.et al.: "Evidence for lateral association of nascent cellulose sheets synthesized by cellulose-synthesized by cellulose-sunthesizing terminal complexes."Memoirs of the Faculty of Science,Kochi Univ.,Ser.D(Biol.). 19. 1-5 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 奥田一雄: "藻類のセルロース合成"電子顕微鏡. 34. 81-86 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Chi E-S.et al.: "Immunogold-labeling analysis of alginate distributions in the cell walls of chromophyte algae."Phycological Research. 47. 53-60 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sekida S.et al.: "An improved method for fixing dinoflagellate non-motile cells for electron microscopy."Memoirs of the Faculty of Science,Kochi Univ.,Ser.D(Biol.). 20. 11-15 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sekida S.et al.: "Direct evidence for cellulose microfibrils present in thecal plates of the dinoflagellate Scrippsiella hexapraecingula."Hikobia. 13. 65-69 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 奥田一雄・峯一朗: "藻類セルロース生合成" Cellulose Comunications. 4・3. 101-107 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] K Okuda,T Tamura,I Mine: "Evidence for lateral association of nascent cellulose sheets synthesized by cellulose-synthesizing terminal complexes." Men.Fac.Sci.Kochi Univ.Ser.D(Biol.). 19. 1-4 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] E-S Chi,EC Henry,H Kawai,K Okuda: "Immunogold-labeling analysis of alginate distributions in the cell walls of chromophyte algae." Phycological Research. 47 in press. in press (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] S Sekida,T Horiguchi, K Okuda: "Direct evidence for cellulose microfibrils present in thecal plates of the dinoflagellate Scrippsiella hexapraecingula." Hikobia. 13 in press. inpress (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] S Sekida,T Horiguchi, K Okuda: "An improved method for fixing dinoflagellate non-motile cells for electron microoscopy." Mem.Fac.Sci.Kochi Univ.Ser.D(Biol.). 20 in press. in press (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 奥田一雄: "藻類のセルロース合成" 電子顕微鏡. 34 in press. in press (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 奥田一雄・峯一朗: "藻類セルロースの生合成" Cellulose Comunications. 4・3. 101-107 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K Okuda,I Mine,T Morinaga,K Kuwaki: "Cytomorphogenesis in coenocytic green algae.V.Segregative cell division and cortical microtubules in Dictyosphaeria cavernose(Siphonocladales,Chlorophyceae)." Phycological Research. 45. 189-196 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K Okuda,S Ueno,I Mine: "Cytomorphogenesis in coenocytic green algae.IV.The contruction of cortical microtubules during lenticular cell formation in Valonia utricularis." Mem.Fac.Sci.Kochi Univ.Ser.D(Biol.). 18. 17-25 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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