| Project/Area Number |
09650482
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
計測・制御工学
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| Research Institution | HIROSHIMA CITY UNIVERSITY |
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Principal Investigator |
YANO Takuo HIROSHIMA CITY UNIVERSITY,FACULTY OF INFORMATION SCIENCES,DEPARTMENT OF INFORMATION MACHINES AND INTERFACES,PROFESSOR, 情報科学部, 教授 (20182389)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | NEAR-INFRARED SPECTROSCOPY / MUSHROOM / SOLID CULTIVATION / CONTROL OF CULTIVATION |
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| Research Abstract |
For mushroom production process, a near-infrared spectroscopy (NIR) was applied to measurement of cellmass, Ganoderma lucidum, in the cultured materials and to control of the process. Cell mass was conventionally measured by analysing the concentration of glucosamine which is a component of the cell wall. The correlation between the concentration of glucosamine obtained by the conventional method and that of obtained by NIR was examined by multiple regression analysis. The value predicted by NIR was in fairly good agreement with that obtained by the conventional method. This result suggests that NIR is applicable to the prediction of growth rate in the solid culture and gives a useful method for the control of mushroom production process.
Furthermore, NIR was employed for the simultaneous determination of the water and rice bran content in solid media in order to development of an automatic machine for mixing and preparation of solid media. The reflected reys, in the wavelength range between 400 and 2500nm, were measured at Znm intervals. Calibration equations for water and rice bran content were obtained using raw and second-derivative spectra, respectively. For both the water and rice bran content, exellent agreement was obseved between the results of the conventional method and those of NIR method. The content of water and rice bran in the solid media could be analyzed simultaneously by NIR.
The NIR procedure was simple, and the operation time required to determine the concentrations of the constituents was only 5min. These results indicate that NIR may be a useful method for monitoring and control of the mushroom production process.
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