| Project/Area Number |
09650870
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物・生体工学
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| Research Institution | Niigata University |
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Principal Investigator |
TANIGUCHI Masayuki Niigata University Engineering Professor, 工学部, 教授 (00163634)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Co-Culture / Propionibacterium / Bifidobacterium / Growth factor / Biorecactor / Probiotics |
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| Research Abstract |
1. Several species of Propionibacterium produced bifidogenic growth stimulator (BGS), that is, 2-amino-3-carboxy- l, 4-naphthoquinone in culture broth. A bioassay system for BGS activity using Bifidobacterium longum as a test microorganism was established.
2. Propionibacterium freudenreichii preferred lactic acid to sugars such as lactose and glucose as a carbon source. Therefore, P.freudenreichii grew well in a co-culture of P.freudenreichii and B.adolescentis, where lactic acid was produced from glucose by B.adolescentis.
3. A novel bioreactor system with two fermentors and two microfiltration modules was developed for efficient production bifidobacterial cells by co-culture of P.freudenreichii and B.adolescentis as well as clarification of interactions between the two bacteria.
4. The proposed co-culture system allowed the successful exchange of culture medium between two fermentors. When P.freudenreichii and B.adolescentis were cultivated individually in the new co-culture system, the amount of bifidobacterial cells produced was twice as high as that with batch culture.
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