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Preparation of a model system for biological solutions and its industrial applications

Research Project

Project/Area Number 09650995
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 高分子構造・物性(含繊維)
Research InstitutionNagoya Institute of Technology

Principal Investigator

KINOSHITA Takatoshi  Nagoya Institute of Technology, Faculty of Engineering, Associate Professor, 工学部, 助教授 (60135407)

Co-Investigator(Kenkyū-buntansha) YOSHIMIZU Hiroaki  Nagoya Institute of Technology, Faculty of Engineering, Assistant Professor, 工学部, 助手 (10240350)
TSUJITA Yoshiharu  Nagoya Institute of Technology, Faculty of Engineering, Professor, 工学部, 教授 (70016591)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsamphiphilic / polypeptides / poly (ethylene glycol) / micelles / solubilization behaviors / orderly packed micelles / plane solutes / model system for biological solutions / ポリペプチド
Research Abstract

Amphiphilic polypeptides, poly( gamma-methyl L-glutamate)-block-poly(ethylene glycol), PMLG-PEG, and poly(gamma -benzyl L-glutamate)-block-poly(ethylene glycol), PBLG-PEG, were prepared and their aggregation structure and solubilization behaviors were investigated. These amphiphilic polypeptides were soluble in water to form spherical aggregates in which the hydrophobic alpha -helical peptide tail was orderly packed in the hexagonal manner. The solubilization of a plane solute, 1-naphthol, into these aggregates was characterized by semi- equilibrium dialysis (SED) method. Values of the solubilization equilibrium constant(K) decrease as the mole fraction of 1-naphthol in the aggregate (X) increases, And the solubilization isotherm of PBLG-PEG-1-naphthol system is higher as compared with that of PMLG-PEG-1-naphthol system. That is, the aromatic-aromatic interaction in the former system may enhance the solubilization affinity between the aggregate and solute. It is shown, therefore, that the interaction between peptide side chains and solutes is the effective factor to control the solubilization behavior of micellar aggregates composed of peptide-based amphiphiles. Thus, we can confirm the selective solubilization behaviors by the peptide- based orderly packed micelles in aqueous solutions, which may be a simple model system of our blood solutions containing albumin for their anomalous solubilization behaviors.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] M.Higuchi 他1名: "“Photocontrol of Molecular Orientation of a Photo-responsive Amphiphilic α-helix in a Lipid Momolayer"" Langmuir. 13.6. 1616-1622 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A.Toyotama 他4名: "“Controllable Orientation of the Peptide-based Surfactant at Air-Water Interface"" Chem.Lett.1997・5. 443-444 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 木下 隆利: "“両親媒性ポリペプチドと分子膜システム"" 高分子. 46・5. 335 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Kinoshita 他3名: "“The Molecular Orientation of a Peptide-based Amphiphile at Hexane/Water Interface"" Chem.Lett.1997・8. 745-746 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 広田安信 他4名: "“α-ヘリックス鎖に沿ったクラウンエーテルの一次元的配列制御とその機能"" 高分子論文集. 54・12. 966-973 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Kinoshita: "“Photoresponsive Membrane Systems"" Photochem.Photobiol., B : Biology. 42・1. 12-19 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Higuchi 他1名: "“Photoresponsive behavior of self-assembling systems be amphiphilic α-helix with azobenzene unit"" Photochem.Photobiol., B : Biology. 42・1. 143-150 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Kishihara 他3名: "“Vertical standing of Amphiphilic helical peptides at the hexane-water interface"" Chem.Lett.1998・9. 951-952 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Ihara 他6名: "“Detection of molecular-shape recognition for polycyclic aromatic hydrocarbons by α-helical poly(L-alanine) on silica"" Chem.Lett.1998・10. 963-964 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Kinoshita 他4名: "“The guest-induced oscillation of a monolayer composed of polypeptide containing β-cycldextrin at the terminal"" Chaos. 9 (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 杉山圭 他5名: "“ペプチド系ミセルの構造と可溶化挙動"" 高分子論文集. 55 (印刷中). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 木下 隆利 他16名: "生体工学" 槙書店, 246 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Higuchi, N.Minoura and T.Kinoshita: ""Photocontrol of Molecular Orientation of a Photo-responsive Amphiphilic alpha-helix in a Lipid Momolayer"" Langmuir. Vol.13, No.6. 1616-1622 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A.Toyotama, s.Kugimiya, M.Yonese, T.Kinoshita and Y.Tsujita: ""Controllable Orientation of the Peptide-based Surfactant at Air-Water Interface"" Chem.Lett.No.5. 443-444 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Kinoshita: ""Amphiphilic Polypeptides and Their Molecular Membrane Systems"" Koubunshi. Vol.46, No.5. 335 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Hosokawa, T.Kinoshita, Y.Tsujita and H.Yoshimizu: ""The Molecular Orientation of a Peptide-based pp.Amphiphile at Hexane/Water Interface"" Chem.Lett.No.8. 745-746 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Hirota, F.Nakanishi, T.Kinoshita, Y.Tsujita and H.Yoshimizu: Koubunshi Ronbun-shu. Vol.54, No.12. 966-973 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Kinoshita: ""Photoresponsive Membrane Systems"" Photochem.Photobiol., B : Biology. Vol.42, No.1. 12-19 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Higuchi and T.Kinoshita: ""Photoresponsive behavior of self-assembling systems by amphiphilic alpha-helix with azobenzene unit"" Photochem.Photobiol., B : Biology. Vol.42, No.1. 143-150 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Kishihara, T.Kinoshita, T.Mori and Y.Okahata: ""Vertical standing of Amphiphilic helical peptides at the hexane-water interface"" Chem.Lett.No.9. 951-952 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Ihara, N.Nakanishi, T.Sagawa, C.Hirayama, T.Sakurai, T.Kinoshita and Y.Tsujita: ""Detection of molecular-shape recognition for polycyclic aromatic hydrocarbons by alpha-helical poly (L-alanine) on silica"" Chem.Lett.No.10. 963-964 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Kinoshita, T.Doi, A.Kato, Y.Tsujita and Yoshimizu: ""The guest-induced oscillation of a monolayer composed of polypeptide containing beta-cycldextrin at the terminal"" Chaos. Vol.9, No.2 (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Sugiyama, T.Sakurai, T.Yamamoto, T.Kinoshita, Y.Tsujita and H.Yoshimizu.: ""The Structure and Solubilization behaviors of Peptide-based Micelles"" Koubunshi Ronbun-shu. Vol.55 (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Kinoshita 他5名: "The guest-induced oscillation of a monolayer couposed of polypeptide coutaining β-cyclodextrin at the terminal" Chaos. 9・2. in press. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Ihara 他6名: "Detection of Molecular-Shope Recognition for Polycyclic Aromatic Hydrocarbons by α-Helical Poly(L-Alanine)an Silica" Chem.Lett.1998・10. 963-964 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Kishihara 他3名: "Vertical standing of Amphiphilic Helical Peptides at Hexane-Water Interface" Chem.Lett.1998・9. 951-952 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Higuchi 他1名: "Photoresponsive behavior of self-assembling systems by amphiphilic α-helix with azobenzene unit" Photochem.Photobiol.,B:Biology. 42・1. 143-150 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Kinoshita: "Photoresponsive Membrane Systems" Photochem.Photobiol.,B:Biology. 42・1. 12-19 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 木下隆利 他: "化学工学の進歩32 生体工学" 槇書店, 246 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T. Kinoshita 他2名: "Plotocentrol of Molecular Orientatim of a Pliutrespuasive Amphiphilic dehelix in a Lipid Monolayer" Lang muir. 13・6. 1616-1622 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Kinoshita 他4名: "Controllable Orientation of the Peptide-based Surfactant at air-Water Interface" Chem Lett. 1997・5. 443-444 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T. Kinoshita 他3名: "The Moledular Orientation of a Peptide-based Anphiphile at Hexane-Water Interface" Chem.Lett. 1997・8. 745-746 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 木下隆利: "両親媒性ポリペプチドと分子膜システム" 高分子. 46・5. 335- (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T. Kinoshita: "Photorosponsire Mewbrane Systems" J.Photochem.& Photobiol.B. 42・1. 12-19 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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