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Reguratory mechanisms of seed germination in rice

Research Project

Project/Area Number 09660004
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionNagoya University

Principal Investigator

YAMAGUCHI Jyunji  Nagoya University Bioscience Center, Associate Professor, 生物分子応答研究センター, 助教授 (10183120)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsalpha-amylase / transcriptional regulation / particle gun bombardment / hexose kinase / sugar sensor / cis-acting elements / G motif / GATA motif / ヘキソ-スキナーゼ / βモチーフ / 糖シグナル / オオムギ / ジベレリン / クロストーク / シグナル伝達 / ヘキソキナーゼ / 胚
Research Abstract

There is increasing evidence showing that in rice alpha-amylase gene expression is controlled not only by the classical hormonal regulation, but also by feed-back metabolite repression. In rice, modulation of alpha-amylase genes by carbohydrates and other metabolite is well described. At least ten genes encode for alpha-amylase isoforms in rice, but two of them are strongly under the control of sugar level, namely RAmy3D and RAmy3E.While the expression of the RAmy3D gene is restricted to the embryo, Ramy1A gene is expressed in both the embryo and aleurone layers. We used a transient expression system (rice embryos) to study the cis-acting elements involved in the sugar repression of the Ramy1A and RAmy3D promoter activity. Gibberellin-response cis-elements of GARE (TAACAAA) and pyrimidine box (CCTTTT) of Ramy1A gene promoter were partially involved in the sugar regulation (Morita et al. 1998). On the contrary, Site-directed mutagenesis of the 50-bp nucleotide sequences from -172 to -123 for RAmy3D gene promoter revealed that consensus sequences of G motif (TACGTA) and TATCCA T/C motif (GATA motif as its antisense sequence) are responsible for sugar repression.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (64 results)

All Other

All Publications (64 results)

  • [Publications] Guglielminetti L: "Effects of anoxia on sucrose degrading enzymes in cereal seeds." J.Plant Physiol.150. 251-258 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Perata P: "Sugar repression of gibberellin-dependent signaling pathway in barley embryos." Plant Cell. 9. 2197-2208 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sugimoto N: "Temporal and spatial expression pattern of rice α-amylase gene RAmy1A during seed development." Rice Genet.Newslett.,. 14. 148-150 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsukura C: "The first leaf sheath growth promoted by gibberellins in dwarf mutant of rice." Rice genet.Newslett.,. 14. 150-153 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ikeda A: "Characterization and expression of GASR1,a root-specific GAST1-like protein in rice." Rice Genet.Newslett.,. 14. 153-155 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Loreti E: "Effect of anoxia on gibberellic acid-induced protease and β-amylase processing in barley seeds." J.Plant Physiol.,. 152. 44-50 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Morita A: "Functional dissection of a sugar-repressed α-amylase gene(RAmy1A) promoter in rice embryos." FEBS Lett.423. 81-85 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sugimoto N: "Temporal and spatial expression pattern of α-amylase gene during germination in rice and barley." Plant Cell Physiol.,. 39. 323-333 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Umemura T: "Sugar sensing and α-amylase gene repression in rice embryos." Planta,. 204. 420-428 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsukura C: "Leaf sheath growth promoted by gibberellins in dwarf mutant of rice." Planta,. 205. 145-152 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toyofuku K: "Promoter elements required for sugar-repression of the RAmy3D gene for α-amylase in rice." FEBS Lett.,. 428. 275-280 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mitsunaga S: "Detection of proteins binding to the promoter region DNA using a nonradioactive gel-retardation assay." Biosci.Biotech.Biochem.62. 1812-1814 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamaguchi J: "Analysis of embryo-specific α-amylase using isolated mature rice embryos." Breeding Sci.48. 365-370 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamaguchi J: "Characterization of β-amylase and its deficiency in various rice cultivars." Theor.Appl.Genet.98. 32-38 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 豊福恭子: "α-アミラーゼ遺伝子の発現制御 -植物ホルモンと糖によるシグナル伝達過程を解明のための分子指標として-," 植物の化学調節,32 : 132-143, 12 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山口淳二: "α-アミラーゼの誘導" 今関英雅,柴岡弘郎編,植物ホルモンと細胞の形,学会出版センター,大阪,43-52, 10 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Guglielminetti L: "Effects of anoxia on sucrose degrading enzymes in cereal seeds." J.Plant Physiol. 150. 251-258 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Perata P: "Sugar repression of gibberellin-dependent signaling pathway in barley embryos." Plant Cell. 9. 2197-2208 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sugimoto N: "Temporal and spatial expression pattern of rice alpha-amylase gene RAmy1A during seed development." Rice Genet.Newslett.14. 148-150 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ikeda A: "Characterization and expression of GASR1, a root-specific GAST1-like protein in rice." Rice Genet.Newslett.14. 150-153 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsukura C: "The first leaf sheath growth promoted by gibberellins in dwarf mutant of rice." Rice Genet.Newslett.14. 153-155 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Morita A: "Functional dissection of a sugar-repressed alpha-amylase gene (RAmy1A) promoter in rice embryos." FEBS Lett.423. 81-85 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sugimoto N: "Temporal and spatial expression pattern of alpha-amylase gene during germination in rice and barley." Plant Cell Physiol.39. 323-333 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Loreti E: "Effect of anoxia on gibberellic acid-induced protease and beta-amylase processing in barley seeds." J.Plant Physiol.152. 44-50 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Umemura T: "Sugar sensing and alpha-amylase gene repression in rice embryos." Planta. 204. 420-428 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsukura C: "Leaf sheath growth promoted by gibberellins in dwarf mutant of roce." Planta. 205. 145-152 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toyofuku K: "Promoter elements required for sugar-repression of the RAmy3D gene for alpha-amylase in rice." FEBS Lett.428. 275-280 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mitsunaga S: "Detection of proteins binding to the promoter region DNA using a nonradioactive gel-retardation assay." Biosci.Biotech.Biochem.62. 1812-1814 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamaguchi J: "Analysis of embryo-specific alpha-amylase using isolated mature rice embryos." Breeding Sci.48. 365-370 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsukura C: "Starch degradation during gibberellin-induced leaf sheath growth in rice." Rice Genet.Newslett.15(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toyofuku K: "Abscisic acid does not affect sugar-repression of rice alpha-amylase gene, RAmy3D." Rice Genet.Newslett.15(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamaguchi J: "Characterization of beta-amylase and its deficiency in various rice cultivars." Theor.Appl.Genet.98. 32-38 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takeuchi W: "Reducing agents-mediated solubilization and activation of debranching enzyme by reducing reagent in rice flour." Biosci.Biotechnol.Biochem.63(3) : (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Guglielminetti L: "Effects of anoxia on sucrose degrading enzymes in cereal seeds." J.Plant Physiol.150. 251-258 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Perata P: "Sugar repression of gibberellin-dependent signaling pathway in barley embryos." Plant Cell. 9. 2197-2208 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sugimoto N: "Temporal and spatial expression pattern of rice α-amylase gene RAmy1A during seed development." Rice Genet.Newslett.14. 148-150 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsukura C: "The first leaf sheath growth promoted by gibberellins in dwarf mutant of rice." Rice Genet.Newslett.14. 150-153 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ikeda A: "Characterization and expression of GASR1, a root-specific GAST1-like protein in rice." Rice Genet.Newslett.14. 153-155 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Loreti E: "Effect of anoxia on gibberellic acid-induced protease and β-amylase processing in barley seeds." J.Plant Physiol.152. 44-50 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Morira A: "Functional dissection of a sugar-repressed α-amylase gene (RAmy1A) promoter in rice embryos." FEBS Lett.423. 81-85 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sugimoto N: "Temporal and spatial expression pattern of α-amylase gene during germination in rice and barley." Plant Cell Physiol.39. 323-333 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Umemura T: "Suger sensing and α-amylase gene repression in rice embryos." Planta. 204. 420-428 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsukura C: "Leaf sheath growth promoted by gibberellins in dwarf mutant of rice." Planta. 205. 145-152 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toyofuku K: "Promoter elements required for sugar-repression of the RAmy3D gene for α-amylase in rice." FEBS Lett.428. 275-280 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mitsunaga S: "Detection of proteins binding to the promoter region DNA using a nonradioactive gel-retardation assay." Biosci.Biotech.Biochem.62. 1812-1814 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamaguchi J: "Analysis of embryo-specific α-amylase using isolated mature rice embryos." Breeding Sci.48. 365-370 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamaguchi J: "Characterization of β-amylase and its deficiency in various rice cultivars." Theor.Appl.Genet.98. 32-38 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 豊福恭子: "α-アミラーゼ遺伝子の発現制御-植物ホルモンと糖によるシグナル伝達過程を解明のための分子指標として-" 植物の化学調節,32 : 132-143, 12 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山口淳二: "α-アミラーゼの誘導" 今関英雅,柴岡弘郎編,植物ホルモンと細胞の形,学会出版センター,大阪,43-52, 10 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Perata P: "Sugar repression of gibberellin-dependent signaling pathway in barley embryos." Plant Cell. 9. 2197-2208 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Guglielminetti L: "Effects of anoxia on sucrose degrading enzymes in cereal seeds." J.Plant Physiol.150. 251-258 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Umemura T: "Sugar sending and α-amylase gene repression in rice embryos." planta. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsukura C: "Leaf sheath growth promoted by gibberellins in dwarf mutant of rice." planta. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sugimoto N: "Temporal and spatial expression pattern of α-amylase gene during germination in rice and barley." Plant Cell Physiol.39(3月号)(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Morita A: "Functional dissection of a sugar-repressed α-amylase gene (RAmylA) promoter in rice embryos." FEBS Lett.(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Loreti E: "Effect of anoxia on gibberellic acid-induced protease and β-amylase processing in barley seeds." J.Plant Physiol.(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ikeda A: "Characterization and expression of GASR1, a root-specific GAST1-like protein in rice." Rice Genet.Newslett.

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsukura C: "The first leaf sheath growth promoted by gibberellins in dwarf mutant" Rice genet.Newslett.

    • Related Report
      1997 Annual Research Report
  • [Publications] Sugimoto N: "Temporal and spatial expression pattern of rice α-amylase gene RAmy1A during seed development." Rice Genet.Newslett.

    • Related Report
      1997 Annual Research Report
  • [Publications] 豊福恭子: "α-アミラーゼ遺伝子の発現制御-植物ホルモンと糖によるシグナル伝達過程を解明のための分子指標として-" 植物の化学調節. 32. 132-143 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 山口淳二: "植物化学調節実験マニュアル,全国農村教育協会" α一アミラーゼ誘導検定法, 6 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 山口淳二: "植物化学調節実験マニュアル,全国農村教育協会" アブシジン酸によるα-アミラーゼ誘導の阻害, 3 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 山口淳二: "植物化学調節実験マニュアル,全国農村教育協会" レポーター遺伝子の導入とその応用, 5 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 山口淳二: "植物ホルモンと形,学会出版センター" α-アミラーゼの誘導, 10 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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