| Project/Area Number |
09660006
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| Research Category |
Grant-in-Aid for Scientific Research (C).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Kagoshima University |
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Principal Investigator |
TAURA Satoru Kagoshima University, Gene Research Center, Associate Professor, 遺伝子実験施設, 助教授 (80216598)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Ipmoea batatas / Meloidogyne incognita / wild spices / resistance / maternal effect / RAPD marker / transformation / plant regeneration |
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| Research Abstract |
In order to apply the resistance genes of sweet potato (Ipomoea batatas) and its related wild species to rot knot nematode (Meloidogyne incognita) for breeding, the following several studies were tried and produced results.
1. Inheritance of resistant in cultivars : F_1 plants from reciprocal crossing between resistant and susceptible cultivar were inoculated with root knot nematode and segregated resistant plants. Maternal effect on resistance was observed in that two cross combinations.
2. Wild species classification : 93 wild species which were collected at central south America from 1956 to 1986 were classified 4 genetic groups using 44 characters. The classification coincided with a classification using RAPD markers.
3. Estimation of resistant degree in wild species : Wild diploid species, I trifida (cross compatible to sweet potato) group was estimated with resistant degree by inoculation test.
4. Resistant expression on different conditions : From observation on the number of invaded nematodes between different growth temperature, 25℃ and 32℃, resistant expression depending on growth temperature was recognized. And on the same observation from 6 to 72 hours after inoculation, initiation of resistant expression was different between the tested lines.
5. Search for related resistant region : F_1 plants from crossing between resistant and susceptible line were inoculated and divided resistant from susceptible. PCR was done using bulked DNA from resistant lines and susceptible lines, respectively. Primers (10 or 12 mar) were used in this search. RAPD marker linked with resistance gene hasn't been found yet.
6. Basic research for transformation : Efficient plant regeneration from apical meristem and protoplast were developed, and somatic cell fusion plants were released.
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