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Analysis of genetic differentiations in radish based on the relationship between make sterile cytoplasma and fertility restorer genes

Research Project

Project/Area Number 09660007
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionKyoto Sangyo University

Principal Investigator

NOMURA Tetsuro (1999)  Kyoto Sangyo University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (50189437)

山岸 博 (1997-1998)  京都産業大学, 工学部, 教授 (10210345)

Co-Investigator(Kenkyū-buntansha) TERACHI Toru  Kyoto Sangyo University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (90202192)
野村 哲郎  京都産業大学, 工学部, 助教授 (50189437)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsMale sterile cytoplasm / Restorer gene / Phylogenetic relation / Sequence polymorphism / RAPD marker / Radish / 雄性不稔性細胞質 / 雄性不稔 / ミトコンドリア
Research Abstract

1. Distribution and polymorphisms of the Ogura cytoplasmic male sterile gene, orf138 : The distribution of orf138 was studies using polymerase chain reaction. The orf138 was not observed in other Cruciferous plants than Raphanus. Wild radishes belonging to R.raphanistrum and Japanese wild radish contain this gene widely, while most of cultivated radishes lack it. For radishes having the oft138, nucleotide sequences of the gene were determined. Seven mutations, six were nucleotide changes and one was a deletion of 39bp, were found in 143 plants. By combination of the mutations, 9 types of the orf 138 sequence were found, among them two major types being distributed in both R.raphanistrum and Japanese wild radish. These results clarifies that the orf138 was originated in R.raphanistrum and was transmitted to Japanese wild radish.
2. Distribution and inheritance of the restorer gene : By test-crosses, the fertility restorer gene for the male sterile cytoplasm was found to be distributed generally in wild radishes. In cultivated radishes, most of Japanese cultivars do not have the gene, while European and Chinese cultivars possess it. This fact demonstrates diversified origins of cultivated radishes. The restorer genes found in a European cultivar, R.raphanistrum and a Japanese wild radish are all dominant one locating on an identical locus.
3. Molecular Marker of the restorer gene : To find molecular markers linked to the restorer gene found in a European cultivar, bulked segregant analysis was applied to a F2 population segregating for the male sterility. Seven RAPD markers were identified, and one of them (OPH11210) was linked tightly to the gene. A few RaPD markers were converted into SCAR markers which are detectable easily and stably.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Hiroshi Yamagishi: "Molecular and biological studies on male-sterile cytoplasm in the Cruciferae IV.Ogura-type cytoplasm found in the wild radishes, Raphanus raphanistrum"Plant Breeding. 116. 323-329 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hiroshi Yamagishi: "Genetic relationships among Japanese wild radishes (Raphanus sativus f.raphanistroides Makino), cultivated radishes and R.raphanistrum revealed by RAPD analysis"J. Japan. Soc. Hort. Sci.. 67. 526-531 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hiroshi Yamagishi: "Distribution and allelism of restorer genes for Ogura cytoplasmic male sterility in wild and cultivated radishes"Genes & Genetic Systems. 73. 79-83 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 山岸 博: "アブラナ科各種植物における細胞質雄性不稔遺伝子の分布の検索"京都産業大学国土利用開発研究所紀要. 20. 145-155 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Seiji Murayama: "Identification of RAPD and SCAR markers linked to restorer gene for Ogura cytoplasmic male sterility in radish (Raphanus sativus L.) by bulked segregant analysis"Breeding Science. 49. 115-121 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hiroshi Yamagishi: "Molecular and biological studies on male-sterile cytoplasm in the Crucifevae "IV". Ogura-type cytoplasm found in the wild radishes, Raphanus raphanistrum"Plant Breeding. 116. 323-329 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hiroshi Yamagishi: "Genetic relationships among Japanese wild radishes (Raphanus sativus f. raphanistroides Makino), cultivated radishes and R.raphanistrum revealed by RAPD analysis"J.Japan Soc. Hort.Sci. 67. 526-531 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hiroshi Yamagishi: "Distribution and allelism of restorer genes for Ogura cytoplasmic male sterility in wild and cultivated radishes"Genes & Genetic Systems. 73. 79-83 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hiroshi Yamagishi: "Detection of the cytoplasmic male sterile genes, orf138, orf263 and orf224, in Crucifereous plants by polymerase chain reactions"Bull. of the Institute for National Land Utilization Development, Kyoto Sangyo Univ.. 20. 145-155 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Seiji Murayama: "Identification of RAPD and SCAR markers linked to a restorer gene for Ogura cytoplasmic male sterility in radish (Raphanus sativus L.) by bulked segregant analysis"Breeding Science. 49. 115-121 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Seiji Murayama: "Identification of RAPD and SCAR markers linked to a restorer gene for Ogura cytoplasmic male sterility in radish (Raphanus sativus L) by bylked segregant analysis."Breeding Science. 49. 115-121 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 山岸 博: "アブラナ科各種植物における細胞質雄性不稔遺伝子の分布の検索"京都産業大学国土利用開発研究所紀要. 20. 145-155 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] YAMAGISHI,H.: "Distribution and allelism of restorer genes for Orura cytoplasmic male sterility in wild and cultinated radishes" Genes & Genetic Systems. 73. 79-83 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山岸 博: "各種アブラナ科植物における細胞質雄性不稔遺伝子分布の検索" 京都産業大学 国土利用開発研究所 紀要. 20,(印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] YAMAGISHI,H.: "Phylogenetic studies of Japanese mild radishes (Raphanus satiuus forma raphanistroides Makino) by RAPD analsis" J.Japan.Soc.Hort.Sci.67(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] YAMAGISHI,H.: "Ogura-type cytoplasm found in the will radish,Raphanus raphanistrum" Plant Breeding. 116. 323-329 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 山岸 博: "ダイコン類における細胞質変異の探索と育種的利用" 農業および園芸. 72・9. 959-962 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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