| Project/Area Number |
09660015
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
作物学
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| Research Institution | Konan University |
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Principal Investigator |
TANAKA Osamu Konan University, Faculty of Science, Professor, 理学部, 教授 (90167495)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Masaaki Osaka Prefecture University, Faculty of Agriculture, Professor, 農学部, 教授 (30027198)
AZUMA Jun-ichi Kyoto University, Faculty of Agriculture, Professor, 農学部, 教授 (80115782)
SHIMADA Takiko Ishikawa Agricultural College, Research Institute of Agricultural Resource, Prof, 農業資源研究所, 教授 (20170946)
IMAI Hiroyuki Konan University, Faculty of Science, Lecturer, 理学部, 講師 (40278792)
NAKAMURA Hakobu Konan University, Faculty of Science, Professor, 理学部, 教授 (80068072)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Aluminum borate whiskers / Rice / Silicon carbide whiskes / Transformation / Transgenic plant / 花成反応 / シリコンカーバイトウィスカ- / ほう酸アルミニウムウィスカ- |
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| Research Abstract |
A transformation of a japonica rice (Oryza sativa cv. Notohikari) has been accomplished by the use of silicon carbide whiskes (SCW) or aluminum borate whiskers (ABW) to deliver DNA into the scutellar tissues of embryos isolated from mature seeds. DNA delivery was mediated by mixing in the presence of plasmid DNA and SCW or ABW.Some parameters of DNA delivery employing SCW or ABW were determined in the tissues based on the transient expression of b-glucronidase (GUS) reporter gene. The optimum condition was established as the mixing of 290 ul buffer containing 25 ug DNA, 20 tissues and 2 mg of SCW or 64 mg ABW for 30 min. Under these conditions, we tried to introduce bar gene whose product confers resistance to the bialaphos into the tissues, together with GUS gene, and obtained the bialaphos resistance calli. Transgenic rice plants were regenerated from them. The integration of GUS gene and bar gene were confirmed by GUS histochemical assay and PCR analysis, respectively. Southern hybridization analysis alsoconfirmed the integration of bar gene. These results suggest that a simple and inexpensive DNA delivery procedure via ABW can be useful to produce transgenic rice plants.
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