| Project/Area Number |
09660016
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
園芸・造園学
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
MASUDA Kiyoshi Fac.of Agr., Hokkaido Univ.Assoc.Pro., 農学部, 助教授 (60157203)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Takashi Fac.of Agr., Hokkaido Univ.Inst., 農学部, 助手 (30196836)
HARADA Takashi Fac.of Agr., Hokkaido Univ.Pro., 農学部, 教授 (30001457)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Sex expression / Tissue culture / Spinach / Somatic embryo / Ethylene / Totipotency / 雌雄性 |
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| Research Abstract |
Somatic embryos obtained from the root callus of female or male plants of a oriental variety of spinach (Spinacia oleracea L.) were grown in vitro under long day length until flowering. Depending on the varieties and donor plants, 12.5 to 80.5% of regenerated plants flowered on a growth regulator-free medium. The plants derived from female explants were all female, while those from male donors included andromonoecy. The sex expression was pursued for plants regenerated from the regenerant-derived callus. The populations originating in female regenerants included gynomonoecy up to 63.7%, while 11.8 to 54.9% of those from male regenerants were andromonoecious. The application of gibberellic acid (GA3) elevated the ratio of andromonoecy but did not induce an absolute sex conversion, i.e., to the opposite sex.
The effects of Ethephon, silver nitrate and aminoethoxyvinylglycine (AVG) on the embryogenic callus formation and embryogenesis were examined using tissue cultures of spinach root segments. The application of Ethephon increased the ratio of embryogenic callus formation. AgNO3 and AVG inhibited the embryogenic callus formation. Ethephon applied at 10muM during embryogenesis, however, completely inhibited the development of embryos. In contrast, the application of 10muM AgNO3 remarkably increased the number of embryos.
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