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Regulation of sugar composition in fruit by metabolic engineering

Research Project

Project/Area Number 09660017
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 園芸・造園学
Research InstitutionTohoku University

Principal Investigator

KANAYAMA Yoshinori  Tohoku University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (10233868)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordssugar / fruit / tomato / fructose / fructokinase
Research Abstract

The important quality of fruit is flavor and in most fruit, consumer flavor preferences are dominated by sweetness. There is a difference in sweetness of hexose sugars, with fructose being approximately twice as sweet as glucose. Because many fruit accumulate approximately equal levels of the hexose isomers, fructose and glucose, metabolic engineering to enhance the proportion of fructose has the potential to enhance fruit sweetness. In this work, the expression patterns of two tomato fructokinase genes, Frk1 and Frk2, are described as well as a strategy to suppress their expression in transgenic tomato plants.
In situ hybridization using Frk1 and Frk2 probes showed differential expression of these two genes in tomato fruit. Frk2 mRNA is localized specifically for starch synthesis and seed development in young tomato fruit whereas Frk1 mRNA is widely distributed. The results from northern blot and in situ hybridization suggest that Frk1 plays a primary role in fructosemetabolism in plant cells while Frk2 is localized specifically in cells that import and utilize moresugar such as starch synthesizing cells and developing seed-related cells. An antisense gene was constructed using Frk1 cDNA and E8 promoter that are cloned to pBl121 binary vector. Approximately 50 transformants were grown and fruit were harvested from them. Although the levels of endogenous Frk1 mRNA were decreased in several t ransformants, any changes in fruct okinase activity and fructose content were not detected in the transformants. Therefore transformation works using Frk1 and Frk2 with 35S promoter were carried out. As a result, decreases in fructokinase activity were observed in some transformants with Frk2 antisense genes. The changes in activity possibly affect sugar composition in fruit.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] T.Lee, T.Kato, Y.Kanayama et al.: "The role of indole-3-acetie acid and acid invertase in the development of melon (Cucumis melo.L.cv.Prince) fruit" J.Japan.Soc.Hort.Sci.65・4. 723-729 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Kanayama et al.: "Divergent fructokinase genes are differentially expressed in tomato" Plant Physiol.113・4. 1379-1384 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Kanayama et al.: "Tomato fructokinases exhibit differential expression and substrate regulation" Plant Physiol.117・1. 85-90 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Sakanishi, Y.Kanayama et al.: "Expression of the gene for NADP-dependent sorbitol-6-phesphate dehydrogenase in peach leares of van'ous clerelopmental stages" Plant Cell Physiol.39・12. 1372-1374 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Kanayama: "Mdecular biolohy of sugar metabolism and its regulation in fruit" J.Japan.Soc.Hort.Sci.67・6. 1203-1208 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Lee, T.Kato, Y.Kanayama, H.Ohno, K.Takeno and S.Yamaki: "The role of indole-3-acetic acid invertase in the development of melon (Cucumis melo L.cv.Prince) fruit." J.Japan.Soc.Hort.Sci.65 (4). 723-729 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Kanayama, N.Dai, D.Granot, M.Petreikov, A.Schaffer and A.B.Bennett: "Divergent fructokinase genes are differentially expressed in tomato." Plant Physiol. 113 (4). 1379-1384 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Shiratake, Y.Kanayama, S.Yamaki: "Characterization of hexose transporter for facilitated diffusion of the tonoplast vesicles from pear fruit." Plant Cell Physiol. 38 (8). 910-916 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] J.Ofosu-Anim, Y.Kanayama and S.Yamaki: "Changes in sugar uptake by excised discs and its stimulation by abscisic and indoleacetic acids during melon fruit development" J.Japan.Soc.Hort.Sci.67 (2). 170-175 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Kanayama, D.Granot, N.Dai, M.Petreikov, A.Schaffer, A.Powell and A.B.Bennett: "Tomato fructokinases exhibit differential expression and substrate regulation." Plant Physiol. 117 (1). 85-90 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Sakanishi, Y.Kanayama, H.Mori, K.Yamada and S.Yamaki: "Expression of the gene for NADP-dependent sorbtiol-6-phosphate dehydrogenase in peach leaves of various developmental stages." Plant Cell Physiol. 39 (12). 1372-1374 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Kanayama: "Molecular biology of sugar metabolism and its regulation in fruit." J.Japan.Soc.Hort.Sci.67 (6). 1203-1208 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Kanayama, D.Granotなど: "Tomato fructokinases exhibit differential expression and substrate regulation" Plant Physiology. 117・1. 85-90 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Sakanishi, Y,Kanayamaなど: "Expression of the gene for NADP-dependent sorbitol-6-phospnate clehydrogenase in peach leaves of various developmental stages" Plant Cell Physiology. 39・12. 1372-1374 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Lee: "The role of indole-3-acetic acid and acid invertase in the development of inelon fruit." J.Japan.Soc.Hort.Sci.65・4. 723-729 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Kanayama: "Divergent fructokinase genes are differestially expressed in tomato" Plant Physiology. 113・4. 1379-1384 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Shiratake: "Characterization of hexose transporter for facilitated diffusion of the tonoplast resicles from pear fruit" Plant Cell Physiol. 38・8. 910-916 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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