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Molecular elucidation of race-cultivar specificity in rice blast disease by using the rice mutants.

Research Project

Project/Area Number 09660050
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionSHIMANE UNIVERSITY

Principal Investigator

ARASE Sakae  Shimane University, Life and Environmental Science, Professor, 生物資源科学部, 教授 (40127478)

Co-Investigator(Kenkyū-buntansha) HONDA Yuichi  Shimane University, Life and Environmental Science, Professor, 生物資源科学部, 教授 (40181557)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsrice blast / rice Sekiguchi lesion mutant / gene-for-gene theory / elicitor / inaccessibility / accessibility / 拒否牲 / いもち病 / 宿主特異的毒素 / 遺伝子対遺伝子
Research Abstract

In this study, the production of race-specific elicitors by M.grisea relating to expression of resistance based on gene-for-gene theory in rice blast disease was investigated. Rice leaf-sheaths of cv.Sekiguchi-asahi which had been pre-inoculated with a compatible race of M.grisea were inoculated with a nonpathogen or an incompatible race in the presence of the spore germination fluids from incompatible race of M.grisea. When the penetration and hyphal growth of post-inoculated fungi in leaf-sheath cells were investigated 36 hr after post-inoculation, there is a large difference in infection behaviors of fungi among spore germination fluids. In the case of the spore germination fluid (sgf) from incompatible races of M.grisea germinated on rice leaf-sheaths for 24 hr, penetration and hyphal growth of A.alternata or incompatible race were significantly inhibited. The sgf from incompatible race induced inaccessibility in healthy leaf-sheaths without pre-inoculation with the compatible race of M.grisea. Further, such induced-inaccessibility was also observed by treatment with the sgf from the incompatible race of M.grisea germinated on the susceptible cultivar Mohoto. In the case of spore germination fluid (SGF) from incompatible race of M. grisea germinated on plastic plates, however, above mentioned induced-inaccessibility phenomena were not observed. The elicitor activity of the sgf was not destructed by heat treatment at 120 C for 20 min. From gel filtration and dialysis tests, it was suggested that molecular weight of the elicitors were smaller than 5,000. From extraction test with organic solvents, it was demonstrated that the elicitors were water-soluble. These results indicate that M.grisea spores produce the elicitors concerning to induced inaccessibility only on host tissues regardless of the compatibility between cultivar and fungal race.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Arase et al.: "Host-specific toxins produced by Magnaporthe grisea, Evidence for role as determinants of basic compatibility in blast disease"Proceedings of the 5th international seminar SUBSEC. 4-11 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Arase et al.: "Response of the rice Sekiguchi lesion (sl) mutant, cv. Sekiguchi-himenomochi, to Magnaporthe grisea"Bull. Fac. Life Env. Sci. Shimane Univ.. 4. 1-6 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Arase et al.: "Light-dependent induction of the Sekiguchi lesion formation by Bipolaris oryzae in rice cv. Sekiguchi-asahi"Journal of Phytopathology. 148 (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Arase et al.: "Light-enhanced resistance to Magnaporthe grisea infection in the rice Sekiguchi lesion mutants"Journal of Phytopathology. 148 (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Arase,S., Hayama,T. and Honda Y.: "Host-specific toxins produced by Magnaporthe grisea ; Evidence for role as determinants of basic compatibility in blast disease"Proceedings of the 5ィイD1thィエD1 International Seminar on Sustainable bioproduction systems on environmental conservation, Korea. 4-11 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Arase,S., Fujita,K., Honda,Y., Kadowaki,Y. and Isota,J.: "Response of the rice Sekiguchi lesion (sl) mutant, cv. Sekiguchi-himenomochi, to Magnaporthe grisea"Bull. Fac. Life Env. Sci. Shimane Univ.. 4. 1-6 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Arase,S., Fujita,K., Uehara,T., Honda,Y. and Isota,J.: "Light-enhanced resistance to magnaporthe grisea infection in the rice Sekiguchi lesion mutants"J. Phytopathol.. 148(in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Arase,S., Fukuyama,R. and Honda,Y.: "Light-dependent induction of the Sekiguchi lesion formation by Bipolaris oryzae in rice cv. Sekiguchi-asahi"J. Phytopathol.. 148(in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Arase et al.: "Host-specific toxins produced by Magnaporthe grisea,Evidence for role as determinants of basic compatibility in blast disease"Proceedings of the 5th international seminar SUBSEC. 4-11 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Arase et al.: "Response of the rice Sekiguchi lesion (sl) mutant,cv.Sekiguchi-hmenomochi,to Magnaporthe grisea"Bull.Fac.Life Env.Sci.Shimane Univ.. 4. 1-6 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Arase et al.: "Light-dependent induction of the Sekiguchi lesion formation by Bipolaris oryzae in rice cv.Sekiguchi-asahi"Journal of Phytopathology. 148(in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Arase et al.: "Light-enhanced resistance to Magnaporthe grisea infection in the rice Sekiguchi lesion mutants."Journal of Phytopathology. 148(in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 荒瀬 栄: "植物病害防除戦略としての感染生理学" 日本植物病理学会, 133 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Arase,S.et al.: "Molecular Genetics of Hose-Specific Toxins^* in Plant Disease" K.Kohmoto and O.C.yoder, 印刷中 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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