| Project/Area Number |
09660051
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物保護
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
ICHINOSE Yuki Okayama University, Graduate School of National Science and Technology, Associate Professor, 大学院・自然科学研究科, 助教授 (50213004)
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| Co-Investigator(Kenkyū-buntansha) |
SHIRAISHI Tomonori Okayama University, Faculty of Agriculture, Professor, 農学部, 教授 (10033268)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | elicitor / suppressor / differential screening / defense response / transcription factor / susceptible reaction / jasmonic acid / pea / cDNA / elicitor / suppressor / transcription factor / DNA binding protein / Zn-finger protein / hsr 203 J / PR protein / defferential screening / glutathione-S-trausferase / hsr203J / S-adenosylmethionine / early nodulin |
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| Research Abstract |
Pea blight pathogen, Mycosphaerella pinodes secrets two kinds of signal molecules, elicitors and suppressors in a germination fluid. Elicitors induce defense response non-specifically on plants and suppressors negate the induced defense response on only host plant. In this study, we have promoted to isolate cDNA clones that are induced by elicitor-treatment to totally understand the defense response and by suppressor-treatment to characterize the mechanisms for the establishment of the compatibility by differential screenings.
As elicitor-inducible genes, we have first constructed a cDNA library from polyA+RNA prepared from elicitor-treated pea epicotyls for 5hr. By the differential screening, we have isolated 90 cDNA clones as candidates for elicitor-responsive genes. Sequencing analysis of these clones revealed as follows. 1) M. pinodes elicitor might induce gene expressions of hydroxyproline-rich glycoprotein and peroxidase to strengthen plant cell wall. 2) Putative cDNAs for polygal
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acturonase inhibiting protein and endochitinase demonstrates that these genes might be effective in incompatible interactions. 3) Isolation of many cDNA clones encoding the enzymes for polyamine biosynthesis indicates the importance of polyamine in defense response. 4) A cDNA clone encoding novel Dof type zinc-finger transcription factor binds specially to AAAG sequence present in the promoter region of many defense-related genes. These results indicate that the significance of this protein in the regulation of complex network of transcriptional regulation by the elicitor-mediated activation of a number of defense response-related genes.
On the other hands, as suppressor-responsive genes, cDNA clones encoding homologs of early nodulin, cell wall degrading enzymes and biosynthetic enzyme for jasmonic acid were isolated. Particularly, corresponding mRNA to jasmonic acid biosynthetic enzyme was specifically induced by the inoculation of compatible pathogen in pea leaves. The investigation of these susceptible specific genes might reveal the mechanism of the establishment of compatible interactions. Less
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