| Project/Area Number |
09660053
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物保護
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| Research Institution | HIROSHIMA PREFECTURAL UNIVERSITY |
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Principal Investigator |
OKU Takashi HIROSHIMA PREFECTURAL UNIVERSITY, SCHOOL OF BIORESOURCES, ASSOCIATE PROFESSOR, 生物資源学部, 助教授 (50201992)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Xanthomonas / hrp / pathogenicity / hypersensitive reaction / type III secretion / regulatory gene / molecular biology / typeIII分泌系 / hrpX / AraC / positive regulator / 塩基配列 / アミノ酸配列 / 宿主特異性 / Xanthomonas campestris / Xanthomonas orysae / DNA binding actirity |
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| Research Abstract |
One of the hrp genes hrpX responsible for elicitation of the hypersensitive response and pathogenicity in plant pathogenic Xanthomonas was identified via the transposone Tn4431 mutagenesis. PCR-mediated southern analysis revearled that the hrpX gene is conserved at least 16 pathovars of plant pathogenic Xanthomonas. The predicated amino acid sequences of the HrpX protein revealed similarities (45.96%) to the HrpB protein of Ralstonia solanacearum, which has sequence identity to the transcriptional activator VirF of Yercinia and AraC of Escherichia coli. HrpX may regulate Xanthomonas hrp genes encoded in the hrp cluster since putative DNA binding helix-turn-helix domain present in the carboxy terminal half of HrpX is highly conserved among HrpB, VirF and AraC. DNA binding activity of the HrpX protein overexpressed in E. coli to a certain site of the promoter region of hrpD1, downstream of the PIP-box known in the homologous region in X. campestris pv. vesicatoria, was confermed by the surface plasmon resonance analysis. These findings strongly suggest that the HrpX, necessary for the expression of Hrp phenotype in plant pathogenic Xanthomonas, may regulate the expression of one of the clustered hrp genes, at least hrpD1, however, gel-shifting assay of the DNA-protein complex is still in progress.
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