Hyper Sensitivity of the Brain of Epileptogenic E1 Mice to Vestibular Neuronal Stimulation
| Project/Area Number |
09660078
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | Nagoya University |
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Principal Investigator |
NAKANO Kiwao NAGOYA UNIVERSITY BIOSCIENCE CENTER,ASSOCIATE PROFESSOR, 生物分子応答研究センター, 助教授 (10023433)
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| Co-Investigator(Kenkyū-buntansha) |
YUASA Shigeki CHIBA UNIVERSITY SCHOOL OF MEDICINE,PROFESSOR, 医学部, 教授 (70127596)
KITOH Junzoh NAGOYA UNIVERSITY SCHOOL OF MEDICINE,PROFESSOR, 医学部, 教授 (60022802)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Epilepsy / E1 mice / Quinolonic acid / Neurotoxin / c-fos / Immediate early genes / Tyrosine hydroxylase / Vestibular stimulation |
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| Research Abstract |
The El mice are an established animal model for human epilepsy. These mice manifest seizures in response to some repeated sensory stimuli with rapidly accelerated movements such as being tossed-up or seasaw movements. Although this technique has been traditionally employed to induce seizures in these mice, the meaning of the stimuli remained obscure. Previously we have shown increased synthesis of qumolinic acid, an excitotoxin, with enhanced expression of 3-hydroxyanthranilate 3,4-dioxygenase (3-HAO) in the brain of El mice as compared with that of ddY mice (the "mother strain"). The aim of this study was to examine the effects of tossing-up stimuli on expression of 3-HAO and c-fos, a well known marker of neuronal activation, in the brain of these mice. The results obtained demonstrate that periodically repeated tossing-up stimuli caused increased expression of 3-HAO and c-fos in the brain of El mice. In contrast, the stimuli had no effect on the expressison of these genes in the brain of ddY mice. These phenomena were not consequences of neuronal activation caused by seizures, since c-fos induction occurred only after single tossing-up stimuli, far earlier than the time manifesting seizures. These results suggest that El mice are genetically hyper-sensitive to vestibular stimuli, resulting in enhanced expression of 3-HAO.This may be a genetic basis for the epileptogenesis of the mice.
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Report
(3 results)
Research Products
(4 results)
