| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Research Abstract |
To understand the leptin's action on hypothalamic arcuate nucleus (ARC), Ca^<2+>/calmodulin (CAM) -dependent response in leptin-treated rat ARC was investigated. The in vitro phosphorylation of ARC extracts from leptin-treated rats did not respond to CaM.Although CaM-dependent protein kinase ll activity was not affected by leptin, the gene expression of calcineurin, CaM-dependent phosphatase, increased in leptin-treated rats.
In the purpose of getting tools for understanding the leptin's biological actions, low molecular weight microbial metabolites, which interact with leptin, were screened using a BlAcore instrument (BlAcore2000, Biacore), the surface plasmon resonance sensor. As a results, linoleic acid was isolated as a active compound. It was revealed that the double bond and the carboxyl group of fatty acids are essential for the binding affinity.
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