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The participation of apoptosis in the suppression of the proliferation of human leulemia cells by the plant constituent

Research Project

Project/Area Number 09660135
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 食品科学・栄養科学
Research InstitutionMIE UNIVERSITY

Principal Investigator

KOMIYA Takashi  Mie University Faculty of Bioresources Professor, 生物資源学部, 教授 (60024577)

Co-Investigator(Kenkyū-buntansha) UMEKAWA Hayato  Mie University Faculty of Bioresources Associater Professor, 生物資源学部, 助教授 (90185059)
HIBASAMI Hiroshige  Mie University Faculty of Medicine Professor, 医学部, 教授 (60024642)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordscatechins / theaflavins / honokiol / oleanoic acid / urosolic acid / change of cell molphology / DNA fragmentation / apoptosis / 紅茶成分 / ホウノオオール / ヒト白血病細胞 / 胃ガン細胞 / 渋柿ジュース / アオキの葉 / 胃癌細胞
Research Abstract

Anticanncer drugs used clinically have side effects and tumor cells are resistant to these drugs. Therefore, new type of anticancer drugs are desirable. This study was conducted to find out new compounds having ability of apoptosis, and to determine structure of the compound and its mechanism of antitumor activity. Persimmon extract and its components such as catechins, specially epigallocatechin gallate(EGCG) suppressed the growth of human leukemia. Moreover, green tea extract, EGCG and black tea component, the aflavin also suppressed the proliferation of leukemia cells. By these components morphology of these cells were changed, and apoptotic bodies and DNA fragments were observed. In other experiment, honokiol was isolated from leaves of honoki and investigated the antitumor activity of honokiol. This compound also inhibited the growth of leukemia cells by inducing apoptosis. In the last experiment, oleanoic acid and ursolic acid were isolated from loquat. These compounds inhibited the activity of ornithine decarboxylase but not induce apoptosis in these leukemia cells. These findings suggest the suppression of the growth result from the synthesis of polyamines.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Y.Achiwa: "Inhibitory effects of persimmon (Dlospyros Kaki) extract and related polyphenol compounds on growth of human lympxoid leukemia cells." Biosci.Biotech.Biochem.61. 1099-1101 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Hibasami: "Induction of apoptosis in human stomach cancer cells by greentea cafechins" Oncology Reports. 5. 527-529 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Komiya: "Effect of Oleanoic-and Ursolic Acids Isolated from Loquet (Eriobotrya) on the Growth of Human Lymphoid Leukemia cells" Food Sci.Technol.4. 282-284 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Hibasami: "Blacktea theaflavius induce programmed cell death in cultural human stomach cancer cells" International Journal of Molecular Medicine. 1. 725-727 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Hibasami: "Honokiol induces apoptosis in human lymphoid leukemia Molt 4B cells" International Journal of Molecular Medicine. 2. 671-673 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Umekawa: "EUKARYOTIC DNA POLYMERASE α BY PERSIMMOS EXTRACT AND RELATED POLYPHENOLS" Biochemistry and Molecular Biolog International. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Achiwa, Y., H.Hibasami, H.Katsuzaki, K.Imai and T.Komiya: "Inhibitory effects of persimmon (Diospyros kaki) extract and related polyphenol compounds on growth of human lymphoid leukemia cells." Biosci.Biotech.Biochem.61. 1099-1101 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hibasami, H., T.Komiya, Y.Achiwa, K.Ohnishi, T.Kojima, K.Nakanishi, K.Akashi and Y.Hara: "Induction of apoptosis in human stomach cancer cells by green tea catechins." Oncology Reports. 5. 527-529 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hibasami, H., T.Komiya, Y.Achiwa, K.Ohnishi, T.Kojima, K.Nakanishi, Y.Sugimoto, M.Hasegawa, R.Akatsuka and Y.Hara: "Black tea theaflavins induce programmed cell death in cultured human stomach canncer cells." International Journal of Molecular Medicine. 1. 725-727 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hibasami, H., Y.Achiwa, H.Katsuzaki, K.Imai, K.Okumura, K.Yoshida, K.Nakanishi, T.Kojima, Y.Ishii and T.Komiya: "Honokiol induces apoptosis in human lymphoid leukemia Molt 4B cells." International Journal of Molecular Medicine. 2. 671-673 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Komiya, T., Y.Achiwa, H.Katsuzaki, K.Imai, S.Sakurai, K.Urakawa, K.Ohnishi, T.Adachi, T.Yamada and H.Hibasami: "Effect of Oleanoic-and Ursolic Acids Isolated from Loquat (Eriobotrya) on the Growth of Human Lymphoid Leukemia cells." Food Sci.Technol., int.4 (4). 282-284 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Umekawa, H., Y.Takada, Y.Furuichi, T.Takahashi, Y.Achiwa, T.Komiya and S.Yoshida: "EUKARYOTIC DNA POLYMERASE alpha BY PERSIMMON (DIOSPYROS KAKI) EXTRACT AND RELATED POLYPHENOLS." sBiochemistry and Molecular Biology International. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Hibasami: "Black tea the aflavins induce programined cell death in human stomack cancer cells" International Journal of Molecular Medicine. 1. 725-727 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Komiya: "Effect of Olechoic-and Vrsolicaods Isolated from Loyuat(Eriobotrya)on the Growth if Human Lymphoid Leukemia cells" Food Sci.Technol.,Int.4・4. 282-284 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Umekawa: "Inhibition of Eukargotic DNA Polymerased by persiminon(Diophros kaki)Extract and Related Polyphenols" Biochemistry and Molecular Biology Intenational. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Achiwa: "Inhibition of Leakenia Cell Growth by Persimmon Extract" Biosci.Biotech.Biochem.61,7. 1099-1107 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Hibasami: "Induetion of apoptosis in human cancer cells by greentea catechins" Oncology reports. 5. 527-529 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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