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Breeding and Gene Manipulation for the Sawdust-Based Cultivation of Lentinula edodes

Research Project

Project/Area Number 09660188
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 林産学
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

OHGA Shoji  Fac. Agriculture, Kyushu University, Associate Professor, 農学部, 助教授 (60117075)

Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsShiitake / Extracellular enzyme / mRNA / Spawn strain / Fruit body / Gene / Sawdust-based substrate / Moisture condition / 育種 / 酵素 / RNA
Research Abstract

Transcriptional regulation of laccase and cellulase mRNA were investigated for fruiting on the sawdust-based cultivation of Lentinula edodes.
(Materials and Methods)
1. Twelve spawn strains were examined in this study. KS-3, -4, -16, -23, -46, -56, -58, -60, -67, and -76 (wide-weather strains), KS-12 and -24 (cold weather strains).
2. The sawdust-based substrate cultivation was done according to the standard methods. Activities of laccase and cellulase were measured at the 7 different growth stages. The growth stages were defined to mycelial growth (90-days old), aggregate (94-days old), pin (98-days old), button (100-days old), veil break (102-days old), senescent (104-days old), and second flush (140-days old).
3. Moisture condition of the sawdust-based substrate was adjusted soaking treatment. The moisture condition was evaluated by the water potential (φ) values.
4. Transcriptional regulation of laccase and cellulase genes was measured by the RT-PCR method.
(Results)
Extracellular enzyme … More activities of laccase and cellulase and their transcriptional regulation were investigated at various growth stages in a sawdust-based substrate for Lentinula edodes. Changes of laccase and cellulase activities revealed a clear relationship with fruit body development stages. Laccase and cellulase activities were regulated at the level of gene transcription. The level of laccase mRNA was maximal at the fully colonized stage and declined during fruit body development. Cellulase mRNA began to accumulate at the pin (miniature fruit bodies) formation stage. Cellulase mRNA transcripts were maximally expressed at the veil-break stage of fruit body development. This tendency was clearer in the fruiting cultures with the wide-range-weather strains than in non-fruiting cold-weather strains. Transcription of laccase and cellulase genes was also affected by the water conditions of the sawdust-based substrate. Primordia initiation occurred when the water potential of the medium was high for rapid mRNA transcription by the mycelium. Less

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Ohga, S.: "The Influence of genotype on the Production of Lentinula edodes Grown on Sawdust-Based Substrate"J. Fac. Agr., Kyushu Univ.. 43. 39-45 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohga, S.: "Effect of Water potential on Fruit Body Formation of Lentinula edodes in Sawdust-Based Substrate."J. Wood Sci.. 45. 337-342 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Koo, C. -D., Kim, J. -S., Cho, N. -S., Min, D. -S., Ohga, S.: "Effect of Moisture Content for Mycelial Growth and Primordial Formation of Lentinula edodes in a Sawdust-Based Substrate"Mushroom Sci. Biotechnol.. 7. 169-174 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohga, S., Cho, N.-S., Thurston, C. F., Wood, D. A.: "Transcriptional Regulation of Laccase and Cellulase in relation to Fruit Body Formation in the Mycelium of Lentinula edodes on a Sawdust-Based Substrate."Mycoscience. 41(印刷中). (受理済) (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohga, S.: "The Influence of Genotype on the Production of Lentinula edodes Grown on Sawdust-Based Substrate"J. Fac. Agr., Kyushu Univ.. 43(1/2). 39-45 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohga, S.: "Effect of Water Potential on Fruit Body Formation of Lentinula edodes in Sawdust-based Substrate"J. Wood Sci.. 45(49). 337-342 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Koo, C. -D., Kim, J. -S., Cho, N. -S, Min, D. -S., Oga, S.: "Effect of Moisture Content for Mycelial Growth and Primordial Formation of Lentinula edodes in a Sawdust-Based Substrate"Mushroom Sci. Biotechnol.. 7(4). 169-174 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohga, S.: "Transcriptional Regulation of Laccase and Cellulase in Relation to Fruit Body Formation in the Mycelium of Lentinula edodes on a Sawdust-based Substrate"Mycoscience. 41,accepted (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohga,S.: "Effect of Water potential on Fruit Body Formation of Lentinula edodes in Sawdust-Based Substrate"J.Wood Sci.. 45・4. 337-342 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Koo,C.-D.,Kim,J.-S.,Cho,N.-S.,Min,D.-S.,Ohga,S.: "Effect of Moisture Content for Mycelial Growth and Primordial Formation of Lentinula edodes in a Sawdust-Based Substrate"Mushroom Sci.Biotechnol.. 7・4 印刷中(受理済). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ohga,S.,Cho,N.-S.Thurston,C.F.,Wood,D.A.,: "Transcriptional Regulation of Laccase and Cellulase in Relation to Fruit Body Formation in the Mycelium of Lentinula edodes on a Sawdust-based Substrate"Mycoscience. 41・3 印刷中(受理済). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shoji Ohga: "The Influence of Genotype on the Production of Lentinula edodes Grown on Sawdust-Based Substrate" J.Fac.Agr.,Kyushu Univ.43・1. 39-45 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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