Modification of Functional Properties of Fish Water Soluble Proteins by Phosphorylation
| Project/Area Number |
09660222
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | Tokyo University of Fisheries |
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Principal Investigator |
TANAKA Munehiko Tokyo University of Fisheries, Department of Food Science and Technology, Professor, 水産学部, 教授 (80092592)
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| Co-Investigator(Kenkyū-buntansha) |
田中 宗彦 東京水産大学, 水産学部, 教授 (80092592)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | phosphorylation / Maillard reaction / water soluble proteins / glucose-6-phosphate / functional properties / glucose / blue marlin / メイラ-ド反応 / グルコ-ス-6-リン酸 / グルコ-ス / リン酸化修飾 / 乳化性 / 消化性 / 保水性 / カルシウム溶解性 / 熱安定性 / pH安定性 / グルコース-6ーリン酸 |
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| Research Abstract |
It has been well known that water soluble proteins (WSP) have an adverse effect on the formation of myofibril protein gels, so that washing with water is a critical procedure for the production of surimi. Such processing requires large quantities of water and a considerable amount of WSP are lost. Therefore, it is beneficial to recover and utilize these proteins as foodstuff to increase value and reduce the cost of waste water treatment. Several methods of recovering WSP from waste water of seafood processing plants have been investigated. However, the recovered proteins are mostly used as animal feeds and fertilizer because of the lack of the techniques to utilize them as foodstuff. This is mainly due to the fact that recovered proteins usually have poor functional properties.
In order to improve the functional properties of WSP in this study, the Maillard reaction with glucose-6-phosphate (G6P) was used to elucidate how the functional properties of WSP can be modified in comparison with glucose (G). The following results were obtained.
(1) WSP from the flesh of blue marlin Makaira mazara were modified through the Maillard reaction with G or G6P by heating at 60℃ for up to 12 h. The Maillard reaction of WSP+G6P was found to progress faster than WSP+G. The incorporation of phosphorus into WSP took place mainly during the early stage of the Maillard reaction.
(2) Increasing phosphorus content of WSP brought about the improvements of solubility at pH above 7, thermal stability, emulsifying activity, water holding capacity, digestibility, calcium phosphate-solubilizing property, and antioxidative activity.
(3) The most suitable ratio of G6P to WSP was found out to be approximately 45%.
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Report
(4 results)
Research Products
(10 results)
