• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Divergent genes encoding the third complement component (C3) in carp.

Research Project

Project/Area Number 09660227
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

YANO Tomoki  Faculty of Agriculture, KYUSHU UNIVERSITY Professor, 農学部, 教授 (90038266)

Co-Investigator(Kenkyū-buntansha) NAKAO Miki  Faculty of Agriculture, KYUSHU UNIVERSITY Assistant Professor, 農学部, 助手 (50212080)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsFISH / IMMUNOLOGY / COMPLEMENT / C3 / DIVERSITY / CLONING / PROTEIN / cDNA / アイソタイプ
Research Abstract

We have previously isolated three distinct full-length cDNA clones and five RT-PCR fragments, all of which encode C3, from a single individual of carp. Interestingly, three of the fragments (C3-S, C3-Q1, and C3-Q2) predicted that thioester-associating His residue, which is found in all other C3 reported so far, is replaced by Ser, GIn, and GIn, respectively. Since recombinant human C4B carrying Ser and rat a-macroglobulin carrying Asn at the corresponding position have been reported to show a human C4A-like binding specificity of the thioester, these amino acid substitution may account for the difference in binding specificity to various target surfaces, which has recently been described for teleost C3. In this study, we have isolated cDNA clones corresponding to the C3-S, C3-Q1, and C3-Q2 from carp hepatopancreas library to further characterize their molecular entity.
C3-S (5.0 kbp) contained a single long open reading frame which encodes pre-pro form of C3 and the deduced amino acid sequence showed about 80% identity with other types of carp C3. On the other hand, C3-Q1 contained several stop codons in the region corresponding to B-chain, suggesting that C3-Q 1 would represent the transcript from a pseudogene. Although C3-Q2 (3.8 kbp) had an open reading frame, it lacked the region corresponding to the 330 amino acids in the middle of B-chain and the C3a region. Moreover, C3-Q2 had a GSGEQ sequence at the thioester site instead of GCGEQ, indicating that C3-Q2 unlikely functions as C3. RT-PCR analysis detected C3-Q2 mRNA in hepatopancreas from seven of twelve carp individuals tested. These results suggest that the genes of C3-Q1 and C3-Q2 would be essentially non-functional, but C3-S seems to exist as a functional protein. Isolation of C3-S protein and analyses of its binding specificity are in progress.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Nakao,M. et al.: "Two diverged complement factor B/C2-like cDNA sequences from a teleost, the common carp (cyprinus carpic)." Journal of Immunology. 161・10. 4811-4818 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Endo,Y. et al.: "Two lineages of mannose-binding lectin-associated serine protease(MASP) in vertebrates." Journal of Immunology. 161・10. 4924-4930 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakao, M: "Structural and functional identification of complement components of the bony fish, carp(cyprinus carpic)." Immunological Reviews. 166. 27-38 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakao, M: "Molecular cloning of multiple forms of carp C3" Molecular Immunology. 35・6-7. 388 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Nakao, J.Mutsuro, R.Obo, K.Fujiki, and T.Yano: "MOLECULAR CLONING OF MULTIPLE FORMS CARP C3" Molecular Immunology. 35. 388 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakao,M.et al.: "Two diverged complement factor B/C2-like (DNA sequences from a teleost, the common carp(Cyprinus carpio)." Journal of Immunology. 161・10. 4811-4818 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Endo, Y.et al.: "Two lineages of mannose-binding lectin-associated serine protease(MASP) in vertebrates." Journal of Immunology. 161・10. 4924-4930 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakao,M.: "Structural and functional identification of complement components of the bony fish, carp(Cyprius carpio)" Immunological Reviews. 166. 27-38 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakao,M: "Molecular cloning of multiple forms of carp C3" Molecular Immunology. 36・6-7. 388 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Nakao: "Sequence diversity of cDNA encoding the third component(C3)of carp(Cyprinus carpio)complement" Developmental and Comparative Immunology. 21-2. 144 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Nakao: "Increase in the C3-receptor on carp(Cyprinus carpio)leukocytes after administration of a β-1,3-glucan" Developmental and Comparative Immunology. 21-2. 145 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Endo: "Molecular Evolution of MBP-Associated serine protease (MASP) in vertebrates" Proceeding of Complement Symposium. 34. 69-70 (1997)

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi