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Research storage light environment for quality preservation of micropropagated transplants

Research Project

Project/Area Number 09660280
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物環境
Research InstitutionChiba University

Principal Investigator

KUBOTA Chieri  Faculty of Horticulture, Chiba University, Associate Professor, 園芸学部, 助教授 (00280917)

Co-Investigator(Kenkyū-buntansha) 全 昶厚 (金 昶厚)  千葉大学, 園芸学部, 助手 (90301093)
KOZAI Toyoki  Faculty of Horticulture, Chiba University, Professor, 園芸学部, 教授 (90081570)
CHUN Changhoo  Faculty of Horticulture, Chiba University, Assistant Professor
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordslow temperature storage / plug transplants / light quality / light compensatin point / transplant production / photosynthesis / 環境制御 / マイクロプロパゲーション / 生長制御
Research Abstract

The investigators have been studied on the effects of light intensity, air temperature, photoperiod, and light quality during storage on the growth and quality of the plantlets. One of the important findings is that plantlets respond to light quality even under low temperature conditions. We have found a positive effect of green light for maintaining photosynthetic ability of stored plug plantlets that have a dense canopy. According to data obtained in the first two years on the plantlet responses to light and temperature environments in storage, we have developed mathematical models to predict the dry weight changes during storage as affected by storage environmental conditions. The predicted dry weight changes during storage were well fitted to the measured values under given combinations of air temperature and photosynthetic photon flux. Use of modeling and simulation techniques was shown to be effective for determining storage environmental conditions and for duration of storage.
Using tomato and sweet potato plantlets micropropagated in vitro, we have also examined the effects of sugar concentration on the carbohydrate concentrations of plantlet, since carbohydrate status of the plantlets is a critical factor for storage. Papers are now under preparation for publishing in scientific journals with peer review process.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] 久保田智惠利: "植物組織培養による増殖・苗生産技術の進歩における環境調節技術の役割"農業及び園芸. 72(9). 8-14 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] C.Kubota: "Carbohydrate status and transplant quality of micropropagated broccoli plantlets stored under different light environments"Postharvest Biology and Technology. 12. 165-173 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] G.Niu: "A System for measuring the in-situ CO2 exchange rates of in vitro plantlets"Hort Science. 33(6). 1076-1078 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 久保田智惠利: "種苗工場における苗貯蔵技術"植物工場の最新技術 (SHITA REPORT). 15. 35-43 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] C.Kubota: "Development of a mathematical model for vegestative propagation : Simulated sweetpotato cutting production as affected by propagation methods and environment"Acta Horticulture. 519. 65-72 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] C.Kubota: "Use of transition matrix models for transplant production under controlled environments"ASAE Paper. 995061. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 久保田智惠利: "新園芸学全編 園芸学最近25年の歩み"養賢堂. 695 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kubota,C., N.C.Rajapakse and R.E.Young: "Carbogydrate status and transplant quality of micropropagated broccoli plantlets stored under different light environments"Postharvest Biology and Technology. 12. 165-173 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miyashita,Y., T.Kimura. Y.Kitaya. C.Kubota and T.Kozai: "Effects of red light on the growth and morphology of potato plantlets in vitro : Using light emitting diodes (LEDS) as a light source for micropropagation"Acta Hort. 418. 169-173 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Niu,G., T.Kozai, and C.Kubota.: "A system for measuring the in situ COィイD22ィエD2 exchange rates of in vitro plantlets"Hort Science. 33(6). 1076-1078 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kozai,T., K.Yoshinaga and C.Kubota.: "Raped production of sweetpotato cutting under controlled environment : COィイD22ィエD2 enrichment and cutting preparation affected the growth and production rate"Hort Science. 32(3). 481 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kubota,C., S.Seiyama, K.Sakami and T.Kozai.: "Transplant quality as affected by temperature, light intensity and photoperiod during storage"Hort Science. 32(3). 481 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ubota,C., M.Ezawa, S.B.Wilson and T.Kozai.: "Carbon balance of tomato and sweetpotato plantlets cultured in vitro with different initial sucrose concentrations of the media"Abstr. Ann. Meeting Jap. Soc. Environ Control Biol.& Jap. Agr. Meteorol.. 278-279 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kubota,C.: "Modeling and simulation in transplant production under controlled environment"Abstr.of Intl. Symp.on transplant Production in Closed System for Solving the Global Issues on Environmental Conservation, Food, Resource and Energy, Feb. 28-Mar. 2,2000,Chiba, Japan. 59 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Willson,S.B., C.Kubota, J.W.Heo, N.C.Rajapakse, R.E.Yong and T.Kozai: "Carbohydrate status of micropropagation photoautotorophic and photomixotrophic plantlets"Abstr.of Intl. Symp.on transplant Production in Closed System for Solving the Global Issues on Environmental Conservation, Food, Resource and Energy, Feb. 28-Mar. 2,2000,Chiba, Japan. 22 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kubota,C. and T.Kozai: "Use of Transition matrix models for transplant production under controlled environments"Pater No.995061.ASAE,2950 Niles Rd., St. Joseph, MI49085-9695USA. (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 久保田 智恵利: "植物組織培養による増殖・苗生産技術の進歩における環境調節技術の役割"農業および園芸. 72(9). 8-14 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] C. Kubota: "Carbohydrate status and transplant quality of micropropagated broccoli plantlets stored under different light environments"Postharvest Biology and Technology. 12. 165-173 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] G. Niu: "A system for measuring the in situ CO_2 exchange rates of in vitro plantlets"Hort Science. 33(6). 1076-1078 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 久保田 智恵利: "種苗工場における苗貯蔵技術"植物工場の最新技術(SHITA REPORT). 15. 35-43 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] S.M. A. Zobayed: "Stomatal characteristics and leaf anatomy of potato plantlets cultured invitro photoautstrophic and photomixotrophic conditions"In Vitro Cell. Dev. Biol. Plant. 35. 183-188 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] C. Kubota: "Use of transition matrix models for transplant production under controlled environments"ASAE Paper. 995061. (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 久保田 智恵利: "新園芸学全編 園芸学最近25年の歩み"養賢堂. 695 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 久保田智恵利: "植物組織培養による増殖・苗生産技術の進歩における環境調節技術の役割" 農業および園芸. 72(9). 8-14 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Niu,G.: "A system for measuring The in situ Co_2 exchange rates of in vitro plantlets" Hort Science. 33(6). 1076-1078 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 久保田智恵利: "新園芸学全編 園芸学最近25年の歩み" 養賢堂, 695 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Chieri Kubota: "Carbohydrate status and transplant quality of micropropagated broccli plantlets stored under different light environments" Postharvest Biology and Technology. 12. 165-173 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 久保田智恵利: "農業技術大系-花卉編-第5巻" 農山漁村文化協会, (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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