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Expression of liver specific functions in chicken hepatocytes

Research Project

Project/Area Number 09660322
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionKagoshima University

Principal Investigator

FUJII Makoto  Kagoshima University, Agriculture, Professor, 農学部, 教授 (70041655)

Co-Investigator(Kenkyū-buntansha) HOU De-xing  Kagoshima University, Agriculture, Associate Professor, 農学部, 助教授 (90305160)
井上 祐一  鹿児島大学, 農学部, 助手 (20284911)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1999: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsChicken / Hepatocyte / Primary culture / Serum-free culture / Liver specific function / Depoisoning enzymes / Environmental pollution chemicals / アルブミン / トランスフェリン / 単層培養
Research Abstract

Rats primary hepatocytes have been usually cultured in a 5% COィイD22ィエD2 incubator at pH 7.4. However, we found that chicken hepatocytes were able to cultured in serum-free L-15 medium for a long period in free gas exchange with the atmosphere (without COィイD22ィエD2 gas). The amount of albumin secreted in the medium decreased to low level early, followed by marked increase from day 9 to day 17 of culture. The profile of appearance of albumin m-RNA was very similar to that of albumin protein. Such observation was not observed in rat hepatocytes, showing some difference in the properties of hepatocytes between avian and rat. Therefore, we tried to monitored the effects of enbironmental pollution chemicals on metabolism in liver of wild birds using chicken hepatocytes as a model. 10ィイD1-1ィエD1 M TCDD (tetracholoro-dibenzo-p-dioxin) decreased the expression of albumin, and 10ィイD1-2ィエD1 M TCDD markedly induced the expression of the depoisoning enzyme, P450 CYP1A4.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Fujii et al.: "primary culture of chicken hepatocytes in serun-free medium (pH 7.8) secreted albumin and transferrin for a long period in free gas exchange with the atmosphere"Int. J. Biochem. Cell Biol.. 28. 1381-1391 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujii et al.: "Effect of 350KDa glycoprotein in royal jelly on primary culture of rat hepatocytes"Food Sci. Technol. Int.. 2. 223-225 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujii et al.: "An economical method for primary culture of chicken hepatocytes in serum-free medium"Jpn. Poult. Sci.. 33. 15-22 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujii et al.: "Effect of Kurozu (brewed rice vinegar) on maintenance of primary culture of rat hepatocytes"Food Sci. Technol. Res.. 5. 97-98 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Inoue et al.: "inhibition of the acid lipase activity by apolipoprotein A-I in the presence of lysosomal proteases"Biosci. Biotechnol. Biochem.. 63. 937-939 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujii M., Yoshino I.., Suzuki M., Higuchi T., Mukai S. ,Aoki T. , Fukunaga T. , Sugimoto Y. , Inoue Y. , Kusuda J. , Saeki T . , Sato M. , Hayashi S. ,Tamaki M. , Sugano T.: "Primary culture of chicken hepatocytes in serum-free medium(pH 7.8) secreted albumin and transferrin for a long atmosphere."Int. J. Biochem. Cell Biol.. 28. 1381-1391 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujii M.,Yonekura M.,Higuchi T., Morimitsu K.,Yoshino I., Mukai S., Aoki T., Fukunaga T., Inoue Y., Sato M., Kanaeda J.: "Effect of 350kDa glycoprotein in royal jelly on primary culture of rat hepatocytes"Food Sci. Technol. Int.. 2. 223-225 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujii M., Suzuki M., Yoshino I., Higuchi T., Manaka M., Aoki T., Fukunaga T., Kusuda JH ., Saeki T., Sugimoto Y., Sugano T., Tamaki M.: "An economical method for primary culture of chicken hepatocytes in serum-free medium"Jpn. Poult. Sci.. 33. 15-22 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujii M., Hou D-X ., Arimura M., Chiwata T., Suzuki M. ,Nagano M.: "Effect of Kurozu (brewed rice vinegar) on maintenance of primary culture of rat hepatocytes"Food Sci. Technol. Res.. 5. 97-98 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Inoue Y., Ose T., Mukai S., Ehira S., Yonekura M., Hou D-X., Fujii M.: "Inhibition of the acid lipase activity by apolipoprotein A-I in the presence of lysosomal proteases."Biosci. Biotechnol. Biochem.. 63. 937-939 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujii et al.,: "Primary culture of chicken hepatocytes in serum-free medium(pH7.8) secreted albumin and transferrin for a long period in free gas exchange with the atmosphere."Int,J.Biochem.Cell Biol.. 28. 1381-1391 (1996)

    • Related Report
      1999 Annual Research Report
  • [Publications] Fujii et al.,: "Effect of 350KDa glycoprotein in royal jelly on primary culture of rat hepatocytes"Food Sci.Technol.Int.,. 2. 223-225 (1996)

    • Related Report
      1999 Annual Research Report
  • [Publications] Fujii et al.,: "An economical method for primary culture of chicken hepatoccytes in serum-free medium"Jpn..Poult.Sci.,. 33. 15-22 (1996)

    • Related Report
      1999 Annual Research Report
  • [Publications] Fujii et al.,: "Effect of Kurozu(brewed rice vinegar) on maintenance of primary culture of rat hepatocytes"Food Sci.Technol.Res.,. 5. 97-98 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Inoue et al: "Inhibition of the acid lipase activity by apolipoprotein A-I in the presence of lysosomal proteases."Biosci.Biotechnol.Biochem.,. 63. 937-939 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Fujii et al.,: "Primary culture of chicken hepatocytes in serum-free medium(pH7.8)secreted albumin and transferrin for a long period in free gas exchange with the atmosphere." Int.J.Biochem.Cell Biol.28. 1381-1391 (1966)

    • Related Report
      1998 Annual Research Report
  • [Publications] Fujii et al.,: "Effect of 350KDa glycoprotein in royal jelly on primary culture of rat hepatocytes." Food Sci.Technol.Int.,. 2. 223-225 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] 藤井 信、他: "ニワトリ肝実質細胞の無血清培地での簡便な初代培養法" 日本家禽学会誌. 33. 15-22 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] Fujii et al.,: "Effect of Kurozu(brewed rice vinegar)on maintenance of primary culture of rat hepatocytes" Food Sci.Technol.Res.,. 5. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 藤井 信、他: "ラット肝細胞初代培養法の比較と食品機能解析への応用" 日本農芸化学会 1999年福岡大会. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 藤井 信、他: "ニワトリ初代培養肝細胞に対する環境汚染物質の影響" 日本家禽学会、1999年鹿児島大会. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Fujii M.et al.,: "Primary culture of chicken hepatocytes in serum-free medium (pH7.8)secreted albumin and transferrin for a long period in free gas exchange with the atmosphere." Int.J.Biochem.Cell Biol.,. 28. 1381-1391 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Fujii M.et al.,: "Effect of 350kDa glycoprotein in royal jelly on primary culture of rat hepatocytes." Food Sci.Technol.Int.,. 2. 223-225 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 藤井 信、他: "ニワトリ肝実質細胞の無血清培地での簡便な初代培養法" 日本家禽学会誌. 33. 15-22 (1996)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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