| Project/Area Number |
09660322
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Kagoshima University |
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Principal Investigator |
FUJII Makoto Kagoshima University, Agriculture, Professor, 農学部, 教授 (70041655)
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| Co-Investigator(Kenkyū-buntansha) |
HOU De-xing Kagoshima University, Agriculture, Associate Professor, 農学部, 助教授 (90305160)
井上 祐一 鹿児島大学, 農学部, 助手 (20284911)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1999: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Chicken / Hepatocyte / Primary culture / Serum-free culture / Liver specific function / Depoisoning enzymes / Environmental pollution chemicals / アルブミン / トランスフェリン / 単層培養 |
|---|
| Research Abstract |
Rats primary hepatocytes have been usually cultured in a 5% COィイD22ィエD2 incubator at pH 7.4. However, we found that chicken hepatocytes were able to cultured in serum-free L-15 medium for a long period in free gas exchange with the atmosphere (without COィイD22ィエD2 gas). The amount of albumin secreted in the medium decreased to low level early, followed by marked increase from day 9 to day 17 of culture. The profile of appearance of albumin m-RNA was very similar to that of albumin protein. Such observation was not observed in rat hepatocytes, showing some difference in the properties of hepatocytes between avian and rat. Therefore, we tried to monitored the effects of enbironmental pollution chemicals on metabolism in liver of wild birds using chicken hepatocytes as a model. 10ィイD1-1ィエD1 M TCDD (tetracholoro-dibenzo-p-dioxin) decreased the expression of albumin, and 10ィイD1-2ィエD1 M TCDD markedly induced the expression of the depoisoning enzyme, P450 CYP1A4.
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