• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

A possible new signal trunsduction mechanism of anterior pituitary cells

Research Project

Project/Area Number 09660326
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionKitasato University

Principal Investigator

HASHIMOTO Inoru  Kitasato University, Professor, 獣医畜産学部, 教授 (30050503)

Co-Investigator(Kenkyū-buntansha) KAWAMINAMI Mitsumori  Kitasato University, Associate Professor, 獣医畜産学部, 助教授 (00153007)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥2,800,000 (Direct Cost: ¥2,800,000)
Keywordsannexin V / prolactin / LH / gonadotrope / anterior pituitary / time resolved fluorometric immunoassay / paracrine / LH beta subunit / 組換え蛋白質 / アネキシン5 / 下垂体 / オートクリン
Research Abstract

Physiological importance of a novel calcium-phospholipid binding protein, armexin V.in functions of the anterior pituitary gland was investigated. We synthesized recombinant rat annexin V, and purified it by the affinity to phospholipid membranes and the reverse phase HPLC.When annexin V was applied to the primary culture of rat anterior pituitary cells, it dramatically increased LH release but inhibited prolactin release. These diverse effects were driven by different mechanisms. As anti annexin V itself augmented prolactin release and Indomethacin reversed this effect, annexin V was thought to inhibit prolactin release by inhibiting phospholipase A2 and reducing prostaglandin synthesis in the primary culture. Prostaglandins are shown to stimulate prolactin release in vitro. On the other hand, the stimulating effect of annexin V on LH secretion is specific to gonadotropes. By using semi-quantitative RT-PCR, annexin V was also shown to stimulate LH beta subunit synthesis. Hence, annexin V was shown to enhance the all process of LH secretion, namely from synthesis to release. As we had observed annexin V distributed on gonadotropes by immunocytochemistry, we examined whether gonadoiropes themselves could synthesize annexin V.For this purpose, we utilized clonal cell line of the gonadotropes, alphaT3-1, to examine the expression of annexin V mRNA and found this cell line synthesizes annexin V.This result suggests the pituitary gonadotropes synthesize annexin V.Getting together, annexin V is suggested to function as a factor of the signal transduction to enhance the synthesis and the release of LH at the gonadotropes, and it is supposed to work simultaneously as a paracrine factor to inhibit prolactin cell function.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Kurusu S: "Role of cytosolic phospholipase A2 in eicosanoid generation by corporalutea of pseudopregnant rat : effects of its specific inhibitor" Prostaglandins, Leukotrienes and Essential Fatty Acids. 57(2). 119-124 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kawaminami M: "Immunocytochemical localization of annexin 5, a calcium dependent phospholipid binding protein, in rat endocrine organs" Cell and Tissue Research. 292. 85-89 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kawaminami, M: "Ovariectomy enhances the expression of annexin 5 in the anterior pituitary gland and induces nuclear translocation in rat gonadotrophs" Moll. Cell. Endoc.141. 73-78 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kurusu S: "Expression and cellular distribution of cytosolic phospholipase A2 in the rat ovary" Prostag Leukotr Ess. 58(6). 399-404 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kurusu S: "Involvement of cytosolic phospholipase A2 in the ovulatory process in gonadotropin-primed immature rats" Prostag Leukotr Ess. 58(6). 405-411 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kawaminami, M: "Ovariectomy enhances the expression of annexin 5 in the anterior pituitary gland and induces nuclear translocation in rat gonadotrophs" Moll Cell Endoc. 141. 73-78 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kurusu S: "Cytosolic phospholipase A2 in rat decidual cells : evidence for its role in decidualization" FEBS Letter. 444(2-3). 235-238 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 久留主志朗: "卵巣における細胞質型ホスホリパーゼA2(cPLA2)の発現と機能" Kitasato J. Vet. Med. Ani. Sci. 印刷中. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kurusu S,Noguchi T,Kawaminami M.Hashimoto I: "Role of cytosolic phospholipase A2 in eicosanoid generation by corpora lutea of pseudopregnant rats : Effects of its specific inhibitor" Prostag Leukotr Ess. 57 (2). 119-124 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kawaminami M,Kawamoto T,Tanabe T,Yamaguchi K,Mutoh K-I,Kurusu S,Hashimoto I: "Immunocytochemical localization of annexin 5, a calcium dependent phospholipid binding protein, in rat endocrine organs" Cell and Tissue Research. 292 (1). 85-89 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kawaminami M: "A novel calcium-phospholipid binding protein, annexin V,is a new candidate for a local regulator of pituitary and ovarian function" Kitasato J.Vet.Med.Ani.Sci. 5 (1). 27-33 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kurusu S,Motegi S,Kawaminami M,Hashimoto I: "Expression and cellular distribution of cytosolic phospholipase A2 in the rat ovary" Prostag Leukotr Ess. 58 (6). 399-404 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kurusu S,Iwao M,Kawaminami M.Hashimoto I: "Involvement of cytosolic phospholipase A2 int the ovulatory process in gonadotropin-primed immature rats" Prostang Leukotr Ess. 58 (6). 405-411 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kawaminami M,Yamaguchi K-I,Miyagawa S,Ioka H,Kurusu S,Hashimoto I: "Ovariectomy enhances the expression of aAnnexin 5 in the anterior pituitary gland and induces nuclear translocation in rat gonadotrophs" Moll Cell Endoc. 141. 73-78 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kurusu S,Endo M,Madarame H,Kawaminami M,Hashimoto I: "Cytosolic phospholipase A2 in rat decidual cells : evidence for its role in decidualization" FEBS Letter. 444 (2-3). 235-238 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kurusu S: "Expression and cellular distribution of cytosolic phospholipase A2 in the rat ovary" Prostag Leukotr Ess. 58(6). 399-404 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kurusu S: "Involvement of cytosolic phospholipase A2 in the ovulatory process in gonadotropin-primed immature rats" Prostag Leukotr Ess. 58(6). 405-411 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kawaminami,M: "Ovariectomy enhances the expression of annexin 5in the anterior pituitary gland and induces nuclear translocation in rat gonadotrophs" Moll Cell Endoc. 141. 73-78 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kurusu S: "Cytosolic phospholipase A2 in rat decidual cells : evidence for its role in decidualization" FEBS Letter. 444(2-3). 235-238 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 久留主志朗: "卵巣における細胞質型ホスホリパーゼA2(cPLA2)の発現と機能" Kitasato J.Vet.Med.Ani.Sci. (印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kurusu S: "Role of cytosolic phospholipase A2 in eicosanoid generation by corpora lutea of pseudopregnant rat:effects of its specific inhibitor" Prostaglandins,Leukotrienes and Essential Fatty Acids. 57(2). 119-124 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kawaminami M: "Immunocytochemical localization of annexin 5,a calcium dependent phospholipid binding protein,in rat endocrine organs" Cell and Tissue Research. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kawaminami,M: "Ovariectomy enhances the expression of annexin 5 in the anterior pituitary gland and induces nuclear translocation in rat gonadotrophs" Moll.Cell Endoc.(in press). (1998)

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi