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Genome analysis of T.equigenitalis and the moleculu epidemiology

Research Project

Project/Area Number 09660333
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionAzabu University

Principal Investigator

MATSUDA Motoo  College of Environmental Health Science, Azabu University, Professor, 環境保健学部, 教授 (50139531)

Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsTaylorella-equigenitalis / contagious equine / motutis / pulsed-field gel electrophoresis / restriction enzymes / genome analysis / molecular epidemiology / ApaIとNOTI / Tayloella equigenitalis / ジエノタイピング / ApaIとNotI
Research Abstract

Contagious equine metritis (CEM) is a sexually transmitted bacterial disease of horses caused by Taylorella equigenitalis, a Gram-negative eubacterium. This species was only relatively recently identified. The GEM was first described in England in 1977. Since then it has been detected in many countries, namely Ireland, the USA, Australia, Europe and Japan and in various horses.
The first occurrence of CEM in horses in Japan and the isolation of T.equigenitalis from cervical swabs from mares with endometritis were reported in 1980.
No attempts have been made to biologically and/or serologically type T.equigenitalis so far we are aware before we have started this research project. It would be very important to clarify scientifically the mechanism(s) of occurrence and route(s) of transmission for CEM and the causative agent, T. equigenitalis.
In the present study we analyzed the restricted genomic DNAs from one hundred and several ten isolates of T. equigenitalis isolated from Ireland, the USA, Australia, England, Norway and Japan by pulsed-field gel electrophoresis(PFGE) after separate digestion with ApaI and NotI.
Consequently, ten and several genotypes were detected among strains of T. equigenitalis examined and a Japanese common genotype J for all strains isolated in Japan was for the first time found among a strain in England.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] T.Miyazawa et al.: "Genotyping of isolates of Taylorella equigenitalis from 〜"Vet. Res. Commun.. 19. 265-271 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M.Matsuda et al.: "Profiles of fragments after pulsed-field gel 〜"Microbiol. Res.. 152. 217-220 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M.Matsuda et al.: "Molecular genotyping by pulsed-field gel 〜"Vet. Res. Commun.. 22. 217-224 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M.Matsuda et al.: "Detection of a common genotype among strains of 〜"J. Basic. Microbiol.. 39. 127-130 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M.Matsuda et al.: "Detection of heterogeneous genotypes among 〜"Aust. Vet. J.. 78. 56-57 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] J.E.Moore et al.: "Molecular surveillance of the incidence of Taylorella 〜"Equine Vet. J.. (印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Miyazawa, M.Matsuda et al.: "Genotyping of isolates of Taylorella equigenitalis from thoroughbred brood mares in Japan"Vet Res Commun. 19. 265-271 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M.Matsuda et al.: "Profiles of fragments after pulsed-field gel electrophoresis of cleaved gemonic DAN from strains of Taylorella equigenitalis isolated from horsed in Norway."Microbiol Res. 151. 217-220 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M.Matsuda et al.: "Molecular genotyping by pulsed-field gel electrophoresis of restricted genomic DAN of strains of Taylorella equigenitalis isolated in Ireland and the United States."Vet Res Commun. 22. 217-224 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M.Matsuda et al.: "Detection of a common genotype among strains of Taylorella equigenitalis isolated from thoroughbred horses in Japan between 1994 and 1996."J Basic Microbiol. 39. 127-130 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M.Matsuda et al.: "Detection of heterogeneous genotypes among Australian strains of Taylorella equigenitalis."Aust Vet J. 78. 56-57 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Miyazawa et al.: "Genotyping of isolates of Taylorella equigenitalis〜"Vet Res Commun. 19. 265-271 (1995)

    • Related Report
      1999 Annual Research Report
  • [Publications] M Matsuda et al.: "Profiles of fragments after pulsod-field gel〜"Microbiol Res. 152. 217-220 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] M Matsuda: "Molecular genotyping by pulsed-field gel-〜"Vet Res Commun. 22. 217-224 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] M Matsuda et al.: "Detection of a common genotype among strains〜"J Basic Microbiol. 39. 127-130 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M Matsuda et al.: "Detection of heterogeneous genotypes among〜"Aust Vet J. 78. 56-57 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] J.E Moore et al.: "Molecular surveillance of the incidence of〜"Equine Vet J. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Matsuda et al.,: "Molecular geno typing by pulsed-field gel electrophoresis〜" Vet.Res.Commun.22. 217-224 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Matsuda et al.: "Profiles of fragments after pcelsed-Fieldgel electro-〜" Microbiological Research. 152. 217-220 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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