| Project/Area Number |
09660349
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | NATIONAL INSTITUTE OF INFECTIOUS DISEASES |
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Principal Investigator |
SAKAGUCHI Masahiro NATIONAL INSTITUTE OF INFECTIOUS DISEASES,DEPARTMENT OF IMMUNOLOGY, SENIOR RESEARCHER, 免疫部, 主任研究官 (20170590)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJIMOTO Majime THE UNIVERSITY OF TOKYO,GRADUATE SCHOOL OF AGRICULTURAL AND LIFE SCIENCES, PROFE, 大学院・農学生命科学研究科, 教授 (60163804)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Dog / IgE / Allergen / ダニ |
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| Research Abstract |
In order to identify allergens for canine atopic dermatits in Japan, intradermal skin tests (IDST) and antigen-specific IgE tests were performed on 42 dogs with clinical manifestation of atopic dermatitis. IDST included 26 allergen extracts and antigen-specific IgE test for 24 allergens by Immunodot method were conducted. The results showed that, of the 42 dogs with atopic dermatitis, 29 cases(69%) and and 23 cases (55%) were reactive to house dust mite (HDM) in IDST and the antigen-spcific IgE test, respecitively. Of 42 dogs, 21 cases (50%) and 7 cases (17%) were reactive to Japanese ceda pollen in IDST and antiggen-specific IgE test. These findings suggested that HDM and Japanese cedar pollen were major allergens in dogs with atopic dermatitis in Japan.
Using both in vivo and in vitro tests, we found that 10 dogs with atopic dermatitis were sensitized to Japanese cedar pollen. Ten dogs with clinical manifestation of atoic dermatitis were diagnosed to be sensitized to the pollen based on the results of IDST and antigen-specific IgE test. Peripheral leukocytes from 2 of the 10 dogs showed histamine release for the crude antigen of the pollen. In 5 of the 10 dogs, lymphocytes showed proliferative response to the crude antigen of the pollen. Since these findings are similar to those in human patients with the pollinosis, the pollen-sensitive dogs may be useful for an animal model to develop new immunotherapy for the pollinosis.
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