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Isolation of a resistance gene against potato virus Y from Solanacearum plants

Research Project

Project/Area Number 09660355
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

MASUTA Chikara  Faculty of Agriculture, Hokkaido Univ., Asso.Pro., 農学部, 助教授 (60281854)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsPVY / virus resistance / tobacco / cell-to-cell movement
Research Abstract

Tobacco cultivar VAM is reported to have the recessive potyvirus resistance gene nu alpha. Varied levels of resistance were observed in VAM plants inoculated with Japanese potato virus Y (PVY) isolates. The VAM cultivar was highly resistant to most of the PVY isolates tested and tolerant to three necrotic strain isolates of PVY-T.Based on data obtained from tissue printing and press blotting, the resistance appeared to be mainly at the level of cell-to-cell movement. PVY replicated in VAM protoplasts, but the replication was 30% lower than in susceptible tobacco, suggesting that impairment of replication also contributes to resistance. To identify the viral gene product(s) involved in VAM resistance, we isolated spontaneous resistance-breaking mutants by passing vein-banding (0 strain) isolates several times through VAM plants. By comparing the amino acid sequences of the mutants and their original isolates, we identified a single amino acid substitution in the VPg domain that is correlated with VAM resistance breaking. Together, these results suggest that, in addition to its role in replication, VPg plays an important role in the cell-to-cell movement of PVY.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Chikara Masuta: "Evolution of a quadripartite hybrid virus by interspecific exchange and recombination between replicase components of two related tripartite RVY Viruses" Proc.Natl.Acad.Sci.USA. 95. 10487-10492 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Chikara Masuta: "A single amino acid change in viral genome-associated protein of potato virus Y correlates with resistance breakingin "Virgin A Mutant"tobacco" Phytopathology. 89. 118-123 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Chikara Masuta: "Evolution of quadripartite hybrid virus by interspecific exchange and recombination between replicase components of two related tripartite RNA virases" Proc.Natl.Acad.Sci.USA. 95. 10487-10492 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Chikara Masuta: "A single amino acid change in viral genome-associated protein of potato virus Y correlates with resistance breaking in 'Virgin A Mutant' tobacco" Phytopathology. 89. 118-123 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Chikara Masuta: "Evolution of a quadripartite hydrid virus by interspecific exchange and recombination between replicase components of two reluted tripartite RNA viruses" Proc.Natl.Acod.Sci.USA. 95. 10487-10492 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Chikara Masuta: "A single amino acid change in viral genume-associated protein of potato virus Y corrclates with resistance breaking Virgin A Mutant'tabacco" Phytopathology. 89. 118-123 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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