| Project/Area Number |
09660355
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
MASUTA Chikara Faculty of Agriculture, Hokkaido Univ., Asso.Pro., 農学部, 助教授 (60281854)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | PVY / virus resistance / tobacco / cell-to-cell movement |
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| Research Abstract |
Tobacco cultivar VAM is reported to have the recessive potyvirus resistance gene nu alpha. Varied levels of resistance were observed in VAM plants inoculated with Japanese potato virus Y (PVY) isolates. The VAM cultivar was highly resistant to most of the PVY isolates tested and tolerant to three necrotic strain isolates of PVY-T.Based on data obtained from tissue printing and press blotting, the resistance appeared to be mainly at the level of cell-to-cell movement. PVY replicated in VAM protoplasts, but the replication was 30% lower than in susceptible tobacco, suggesting that impairment of replication also contributes to resistance. To identify the viral gene product(s) involved in VAM resistance, we isolated spontaneous resistance-breaking mutants by passing vein-banding (0 strain) isolates several times through VAM plants. By comparing the amino acid sequences of the mutants and their original isolates, we identified a single amino acid substitution in the VPg domain that is correlated with VAM resistance breaking. Together, these results suggest that, in addition to its role in replication, VPg plays an important role in the cell-to-cell movement of PVY.
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