• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Aiming at a phylogenetic tree of vertebrate photoreceptor cells on the basis of immunocytochemistry

Research Project

Project/Area Number 09670001
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionYamagata University

Principal Investigator

TONOSAKI Akira  Yamagata University School of Medicine, Professor, 医学部, 教授 (90004572)

Co-Investigator(Kenkyū-buntansha) MORI Keiji  Yamagata University School of Medicine, Assistant, 医学部, 助手 (40250914)
遠藤 浩志  山形大学, 医学部, 助手 (90280891)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsretina / lamprey / visual pigment / cytophylogenetic tree / immunohistochemistry / monoclonal antibodies / 松果体 / 共焦点レーザー顕微鏡
Research Abstract

1) Production of monoclonal antibodies against lamprey-retinal homogenate.
By this project, 23 new monoclonal antibodies were added to the series of antibodies we precedingly produced. According to their binding sites, the 23 antibodies were classified as follows : (1) Putative photopigment-specific antibodies including long photoreceptor cell (LPC) specific, Fl, Al and short (SPC) -specific, HI ; (2) LPC- and SPC -inner segment-specific, C12, H7, and F91 ; (3) Entire cell-body (LPC and SPC) -specific, G3, 04, and F92 ; (4) Several antibodies which were characterized by their binding to broad areas of glial processes, D2, D5, G3, D1, F11, A3, H6, D12, and A8 ; and (5) Pigment epithelium-specific, A1. In the present project, D2 was selected and investigated particularyin distal. D2 was found to bind with intermediate-sized filaments of Muller cell of the retina, supporting cells of the pineal and parapineal bodies, and ependymal cells bordering the ventricular system. By Western blotting against retinal homogenate, D2 antibody was enhanced by a remarkable band at 76kD.The nature of the protein is recognized by D2 antibody will be subject of the next project.
2) Electron microscopy of immunogold binding sites.
By freeze replica labeling method, a component of rhodopsin in the form dense IMP gathering more or less with immunogold conjugated H16 antibody. For this method, antibody H16 was most effective, so that it was useful as a standard for the EM immunocytochemistry. For D2, however, was detected on intermediate-sized filaments which were mostly hidden under shadowing deposition.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Saito S, Goto K, Tonosaki A, Kondo H: "Gene cloning and characterization of CDP-diacylglycerol synthase from rat brain." J Bio Chem. 272. 9503-9509 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ishikawa M, Hisatomi O, Tonosaki A, Sakuragi S: "Prefeernce of peanut agglutinin labelling for log-wavelength-sensitive cone photoreceptors in the dace retina." Vision Res. 37. 338-387 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hisatomi O, ishikawa M, Tonosaki A, Tokunaga F: "Characterization of lamprey rhodopsin by isolation lamprey retina and expression in mammalian cells." Photochem photobio. 66. 792-795 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Luo ZW, Kosaka M, Othman T, Piad JN, Cao Y, Lee JB, Matsumoto T, Ohwatari N, Ichinose A, Mori K, Tonosaki A: "Anatomical and neurochemical peculiarities of the pika retina : basis for lack of circadian rhythm of core temperature." Neurosci Lett. 4. 259(1):13-6 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Saito S, Sakagami H, Tonosaki A, Kondo H: "Localization of mRNAs for CDP-diacylglycerol synthase and phosphatidylinositol synthase in the brain and retina of developign and adult rats." Brain Res Dev Brain Res. Sep 10 ; 110(1). 21-30 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tonosaki A, Mori K: "Immunologocal kinship between retina and pineal photoreceptor cells in Lamprey (Lampretra japonica)" Dynamic Cells : Cell Biology of 21st Century. 77-83 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Saito S,Goto K,Tonosaki A,Kondo H: "Gene cloning and characterization of CDP-diacylglycerol synthase from rat brain." J Bio Chem. 272. 9503-9509 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matui H,Hattori R,Watanabe H,Tonosaki A,Hattori T: "Na^+-induced structural change of a soil bacterium, S34, and Ca^+ requirement for preserving its original structure." J Bcteriol. 179. 3350-3353 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ishikawa M,Hisatomi O,Tonosaki A,Sakuragi S: "Prefeernce of peanut agglutinin labelling for log-wavelength-sensitive cone photoreceptors in the dace retina." Vision Res. 37. 338-387 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hisatomi O,Ishikawa M,Tonosaki A,Tokunaga F: "Characterization of lamprey rhodopsin by isolation lamprey retina and expression in mammalian cells." Photochem photobio. 66. 792-795 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Luo ZW,Kosaka M,Othman T,Piad JN,Cao Y,Lee JB,Matsumoto T,Ohwatari N,Ichinose A,Mori K,Tonosaki A: "Anatomical and neurochemical peculiarities of the pika retina : basis for lack of circadian rhythm of core temperature." Neurosci Lett. 4 ; 259(1). 13-6 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Saito S,Sakagami H,Tonosaki A,Kondo H: "Localization of mRNAs for CDP-diacylglycerol synthase and phosphatidylinositol synthase in the brain and retina of developing and adult rats." Brain Res Dev Brain Res. 110(1). 21-30 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Endo H,Sasaki K,Tonosaki A,Kayama T: "Three-dimensional and ultrastructural ICAM-1 distribution in the choroid plexus, arachnoid membrane and dural sinus of inflammatory rats induced by LPS injection in the lateral ventricles." Brain Res. 793(1-2). 297-301 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tonosaki A,Mori K: "Immunologocal kinship between retina and pineal photoreceptor cells in Lamprey(Lampetra japonica)" Dynamic Cells : Cell Biology of 21st Century. 77-83 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Luo ZW,Kosaka M,Othman T,Piad JN,Cao Y,Lee JB,Matsumoto T,Ohwatari N,Ichinose A,Mori K,Tonosaki A: "Anatomical and neurochemical peculiarities of the pika retina: basis for lack of circadian rhythm of core temperature." Neurosci Lett. 4;259(1). 13-16 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Saito S,Sakagami H,Tonosaki A,Kondo H: "Localization of mRNAs for CDP-diacylglycerol synthase and phosphatidylinositol synthase in the brain and retina of developing and adult rats." Brain Res Dev Brain Res. 110(1). 21-30 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Endo H,Sasaki K,Tonosaki A,Kayama T: "Three-dimensional and ultrastructural ICAM-1 distributiion in the choroid plexus,arachnoid membrane and dural sinus of inflammatory rats induced by LPS injection in the lateral ventricles." Brain Res. 793(1-2). 297-301 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tonosaki A,Mori K: "Immunologocal kinship between retina and pineal photoreceptor cells in Lamprey(Lampetra japonica)" Dynamic Cells: Cell Biology of 21st Century. 77-83 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Saito S, Goto K, Tonosaki A, Kondo H: "Gene cloning and characterization of CDP-diacylglycerol synthase from rat brain." J Bio Chem 272,9503-9509,1997.

    • Related Report
      1997 Annual Research Report
  • [Publications] Matui H, Hattori R, Watanabe H, Tonosaki A, Hattori T: "Na^+-induced structural change of a soil bacterium,S34,and Ca^+ requirement for preserving its original structure." J Bcteriol 179,3350-3353,1997.

    • Related Report
      1997 Annual Research Report
  • [Publications] Ishikawa M, Hisatomi O, Tonosaki A, Sakuragi S: "Prefeernce of peanut agglutinin labelling for log-wavelength-sensitive cone photoreceptors in the dace retina." Vision Res 37,338-387,1997.

    • Related Report
      1997 Annual Research Report
  • [Publications] Hisatomi O, Ishikawa M, Tonosaki A, Tokunaga F: "Characterzation of lamprey rhodopsin by isolation lamprey retina and expression in mammalian cells." Photochem photobio 66,792-795,1997.

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi