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Ontogeny and phylogeny of lymphatics and lymphatic stomata of the pleura and diaphragm

Research Project

Project/Area Number 09670005
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionTOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY

Principal Investigator

OHTANI Osamu  Toyama Medical and Pharmaceutical University Faculty of Medicine Professor, 医学部, 教授 (90127548)

Co-Investigator(Kenkyū-buntansha) LI Ruixi  Toyama Medical and Pharmaceutical University Faculty of Medicine Assistant Professor, 医学部, 助手 (20303240)
OHTANI Yuko  Toyama Medical and Pharmaceutical University Faculty of Medicine Assistant Professor, 医学部, 助手 (10313595)
斎藤 麻由  富山医科薬科大学, 医学部, 助手 (70235076)
邵 旭建  富山医科薬科大学, 医学部, 助手 (40281018)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsdiaphragm / pleura / lymphatic vessels / lymphatic stomata / ontogeny / phylogeny / ultrastructure / lymph-angiogenesis / 電子顕微鏡 / リンパ管発生 / 酵素組織化学 / VEGF-C
Research Abstract

We investigated the distribution, ultrastructure, development, and function of the lymphatic system of the pleura and the diaphragm. The results obtained are as follows :
1) When the volume of ascites absorbed into the diaphragmatic lymphatics exceeds lymph volume that can be drained through the draining lymphatic trunks of the diaphragm, part of the ascites can transmit to the pleural cavity. This is at least one mechanism causing a hydrothorax during continuous ambulatory peritoneal dialysis and disease with ascites.
2) Our study has revealed the developmental process of the lymphatics and lymphatic stomata in the rat diaphragm. The lymphatics develop mainly by sprouting and fusing. At early postnatal stages, the lymphatic endothelial cells extend toward the mesothelial cells and form channels or stomata that open into the peritoneal cavity.
3) In the rat costal pleura, we have also revealed the ultrastructure, distribution, and developmental process of the lymphatics and lympyhatic stomata. The lymphatics and the lymphatic stomata in the pleura develop in almost same way as those in the diaphragm.
4) In the bullfrog, the pleuroperitoneal cavity is separated by a thin membrane with numerous stomata from the vast subvertebral lymphatic sinus. We have revealed the ultrastructure of the stomata by electron microscopy.
5) DiI-labeled acetyl-LDL injected into the peritoneal cavity enters the diaphragmatic lymphatics and is taken up by lymphatic endothelial cells. The endothelial cells that took DiI up can be observed clearly under a confocal laser-scanning microscope. This method is quite useful to study the lymphatic system of the diaphragm.
6) At the embryonic and early postnatal stages, there are many migrating lymphatic endothelial cells that do not form channels. This suggests that at these stages the lymphatics are formed by lymph-vasculogenesis and lymph-angiogenesis.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Wang, Q.-X.: "Distribution and ultrastructure of the stomata connecting the pleural cavity with lymphatics in the rat costal pleura"Acta Anatomica. 158. 255-265 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohtani, Y.: "Obliteration of the lymphatic trunks draining diaphragmatic lymph causes peritoneal fluid to enter the pleural cavity"Archives Histology and Cytology. 60. 503-510 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 王 全新: "ラット肋骨胸膜における胸膜腔とリンパ管を直接連絡する小孔の発達"三次元微小解剖. 3. 23-26 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 斎藤 麻由: "ラット肋骨胸膜における胸膜腔とリンパ管を直接連絡する小孔の微細構造"三次元微小解剖. 3. 27-30 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 大谷 修: "横隔膜の微細構造"The Lung Perspectives. 5. 83-87 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shao X.-J.: "Development of diaphragmatic lymphatics : process of their direct connection to the peritoneal cavity"Archives Histology and Cytology. 61. 137-149 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Wang, Q. -X., Ohtani, O., Saitoh, M., and Ohtani, Y.: "Distribution and ultrastructure of the stomata connecting the pleural cavity with lymphatics in the rat costal pleura."Acta Anatomica (Basel). 158. 255-265 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohtani, Y., and Ohtani, O.: "Obliteration of the lymphatic trunks draining diaphragmatic lymph causes peritoneal fluid to enter the pleural cavity."Archives Histology and Cytology. 60. 503-510 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Wang, Q. -X., Saitoh, M., Ohtani, O.: "Development of stomata directly connecting the pleural cavity with lymphatic vessels in the rat costal pleura."3D Microanatomy. 3. 23-26 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Saitoh, M., Wang, Q. -X., and Ohtani, O.: "Ultraxtructure of stomata directly connecting the pleural cavity with lymphatic vesseks in the rat costal pleura."3D Microanatomy. 3. 27-30 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohtani, O.: "Ultrastructure of the diaphragm."The Lung Perspectives. 5. 83-87 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shao, X. -J., Ohtani, O., Saitoh, M., and Ohtani, Y.: "development of diaphragmatic lymphatics : process of their direct connection to the peritoneal cavity."Archives Histology and Cytology. 61. 137-149 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y,Ohtani and O.Ohtani: "Obliteration of the lymphatic trunks draining diaphragmatic lginph causes peritoneal fluid to enter the pleural cavity" Arch.Histol.Cytol.60・5. 503-510 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] X.-J.Shao,O.Ohtani,M.Saitoh and Y.Ohtani: "Development of diaphragmatic lymphatics: the process of their direct connection to the peritoneal cavity" Arch.Histol.Cytol.61・2. 137-149 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] "Distribution and ultrastructure of the stomata connecting the pleural cavity with lymphatics in the rat costal pleura" Acta Anatomica. 158巻4号. 255-265 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yuko Ohtani: "Obliteration of the lymphatic trunks draining diaphragmatic lymph causes peritoneal fluid to enter the pleural cavity" Archives Histology and Cytology. 60巻5号. 503-510 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Xu-Jian Shao: "Development of diaphragmatic lymphatics:process of their direct connection to the peritoneal cavity" Archives Histology and Cytology. 61巻2号(印刷中). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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