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Analysis of spermatogenesis & meiosis using knockout mice

Research Project

Project/Area Number 09670010
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

MORI Chisato  Kyoto Uni.Fac.Med.Associ Pro, 医学研究科, 助教授 (90174375)

Co-Investigator(Kenkyū-buntansha) SHIOTA Kohei  Kyoto Uni.Fac.Med.Professor, 医学研究科, 教授 (80109529)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsspermatogenesis / meiosis / mouse / knockout mouse / heat shock protein / hexokinase / apoptosis / spermiogenesis / アボプトーシス / 解糖系酵素 / 精子 / 繊維鞘
Research Abstract

The developmental program of spermatogenesis is a multi-step process which includes mitotic, meiotic, and post-meiotic phases characterized by dramatic shifts in the patterns of gene expression. We examined the function and expression pattern of spermatogenic cell specific genes (mHkl-s and Hsp 70-2) using knock-out mice.
Unique type 1 hexokinase (HK1) mRNAs encode a spermatogenic cell-specific sequence region (SSR), but not the porin-binding domain (PBD) necessary for HK1 binding to porin on the outer mitochondrial membrane. Our study (Mori et al., 1998) determined the origin of the multiple Hkl-s transcripts in mouse spermatogenic cells and verified that they are translated in mouse spermatogenic cells. It also showed that a single mHkl gene encodes the mHkl transcripts of somatic cells and the mHkl-sa and mHkl-sb transcripts of spermatogenic cells, that alternative exons are used during mHkl gene expression in mouse spermatogenic cells, and that mHKl-S is translated in mouse spermato … More genic cells and is localized mainly with the fibrous sheath in the tail region.
HSP70-2 is a unique member of the mouse 70-kDa heat shock protein family that is synthesized during meiosis in spermatogenic cells. Germ cells in male mice homozygous for a targeted mutation in the Hsp70-2 gene (Hsp7O-2^<-1->) arrest in development and undergo apoptosis at the end of the pachytene spermatocyte stage of meiotic prophase. However, cells with a putative acrosome were present occasionally in histological sections of the testes of juvenile and adult Hsp70-2^<-1-> mice. Our study (Mori et al., 1999) verified that acrosomes were present and investigated the relationship between acrosome formation and the process of meiosis. Our results indicate that not all pachytene spermatocytes in Hsp70-2^<-1-> mice arrest in meiosis, but may divide once or sometimes twice and begin acrosome formation and nuclear condensation. This demonstrates that some aspects of spermatid development can occur without the completion of meiosis in mice. Less

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Mori C. et al,: "Morphological analysis of germ cell apoptosis during postnatal testis development in normal and Hsp70 2 komice" Dev Dyn. 208. 125-136 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakagawa S.et al.: "Spermatogenic cell apoptosis induced by Mitomycin C in the Mouse testis" Toxical Appl Pharmacol. 147. 204-213 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mori C: "Morphological analysis of spermatogenic cell apoptosis" Reprod Biol Update. 229-238 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mori C.et al.,: "Mouse spermatogenic cell-speafic type 1 hexokinase(mHK1-S)transcripts are expressed by alterna fire splicing from mHK1 gene and the HK1-s・・・" Mol.Reprod Dev. 49. 374-385 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin J-H et al.,: "Involvement of germ cell apoptosis in the induction of testicular toxicity following hyroxyurea tretment" Toxical Appl pharmacol. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mori C et al.,: "Completion of meiosis is not required for acrosome formation in HSP70-2 null mice." Biol Reprod. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mori C,Nakamura N,Dix DJ,Fujioka M,Nakagawa S,Shiota K and Eddy EM.: "Morphological analysis of germ cell apoptosis during postnatal testis development in normal and Hsp70-2 knockout mice." Dev Dyn. 208. 125-136 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakagawa S,Nakamura N,Fujioka M and Mori C: "Spermatogenic cell apoptosis induced by mitomycin C in the mouse testis." Toxicol Appl Pharmacol. 147. 204-213 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Dix DJ,Allen JW,Collins BW,Poorman-Allen P,Mori C,Brown PR,Goulding EH,Strong BD and Eddy EM: "HSP70-2 is required for desynapsis of synaptonemal complexes during meiotic prophase in juvenile and adult mouse spermatocytes." Development. 124. 4595-4603 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mori C,Nakamura N,Welch JE,Gotoh H,Goulding EH,Fujioka M and Eddy EM: "Mouse spermatogenic cell-specific type 1 hexokinase (mHk1-s) transcripts are expressed by alternative splicing from the mHk1 gene and the HK1-S protein is localized mainly in the sperm tail." Mol Reprod Dev. 49. 374-385 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin J-H,Mori C and Shiota K: "Involvement of germ cell apoptosis in the induction of testicular toxicity following hydroxyurea treatment." Toxicol Appl Pharmacol. 155. 139-149 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mori C,Allen JW,Dix DJ,Nakamura N,Fujioka M,Toshimori K and Eddy EM: "Completion of meiosis is not required for acrosome formation in HSP70-2 null mice." Biol Reprod. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mori C et al.: "Maphological analysis of gerin cell apoptosis during postvatal Testis development in neural anti Hsp 70・2 knockout mice." Dev Dyu. 205. 125-136 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakagawa S.et al.: "Spermotegenic cell apoptosis induced by Mitoraycin C in the Mouse testis." Toxrial Appl Pharmacol. 147. 204-213 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mori C.: "Morphological analysis of sperintogenic cell apoptosis. TUNEL is a useful tool for detecting testicular toxicity after chemical trestant" Reprod Biol Update. 229-238 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mori C.et al.: "Mouse spermategenic cell Specific type 1 venekinase(mHK1-s)transcripts are expressed by alternative spliing from the mHK1 gene condure HK1-spectein is localized" Mol Repeacl Dev. 49. 374-385 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shin J-H.et al: "Involvement of gerin cell apoptosis in the ireraction of testeciler texocity following Hydroxymia frectuent" Toxical appl pharmacol. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mori C.et al.: "Campletion of meiosis is not required for acressive toxintion in HSP70・2 null mices" Biol.Reprod.(1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 森 千里: "精子形成過程に特異的な遺伝子発現" 細胞. 27. 422-427 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mori,C.et al.: "Testis-specitic expression of mRNAs for a unique human type 1 hexokinase lacking the porin-birding dowain." Mol Reprod Dev. 44. 14-22 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mori,C.et al.: "Morphological analysis of germ cell apoptosis during postnatal testis development in mormal and Hsp70-2 Knockout wice." Dev.Dyn.208. 125-136 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakagawa,S.et al.: "Spermatogemic cell apoptosis induced by Mitomycin C in the Mouse testis." Toxicol Appl pharmacol. 147. 204-213 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mori,C.: "Morphological analysis of Spermatoginic cell apoptosis TUNEL is a useful tool for dutecting testicular toxicityaffer cheuncal freatment" Reprod Biol Update. 229-238 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mori,C.et al.: "mouse spermatogenic cell-specific type 1 hexokinase (mHKl-s) transcripts are expessed by alternative spliuigfrom the mHKl gene and the HKl-s protein is localized mainly ion the sperm tail" Mol Reprod Dev. 49. 374-385 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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