| Project/Area Number |
09670020
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Oita Medical University |
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Principal Investigator |
KATO Seiji Oita Medical University, Department of Human Biology, Professor, 医学部, 教授 (60034956)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMODA Hirosi Oita Medical University, Department of Anatomy, Assistant Professor, 医学部, 助手 (20274748)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | lymphatics / 5'-nucleotidase / microcirculation / histochemistry / blood vessel / alkaline phosphatase / development / diaminopeptidase / 5'-nucleotidase / Alkaline phosphatase / Diaminopeptidase / 循環 |
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| Research Abstract |
The microcirculatory system, lymphatics and blood vessels was histochemically studied in regards to the organ specificity of the structural organization and fine distribution of the lymphatic capillary network. The lymphatics and blood vessels are characterized by an enzyme-histochemical method using 5'-nucleotidase (5'-Nase), alkaline phosphatase (ALPase) and/or diaminopeptidase (DAPase) staining in addition to an immunohistochemical method. The 5'-Nase-positive lymphatic vessels can be distinguished histochemically from arterial and venous vessels based on ALPase and DAPase activity, respectively. The specificity and localization of enzyme reactions were confirmed by comparative histochemical studies of the same specimen under light microscope and scanning or transmission electron microscope. Furthermore, this report presents a new applicability of the backscattered electron imaginings of 5'-Nase reaction for lymphatics and acetyicholinesterase reactions for nerve plexus to scanning electron microscopic histochemistry stained with heavy metal, respectively. These histochemical methods are discussed in relation to their ability to demonstrate the organ specificity of vascular networks under normal and pathological condition.
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