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Molecular and cell biological study on the expression of germ cell function.

Research Project

Project/Area Number 09670021
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionMiyazaki Medical College

Principal Investigator

TOSHIMORI Kiyotaka  Miyazaki Medical College, Anatomy, Professor, 医学部, 教授 (20094097)

Co-Investigator(Kenkyū-buntansha) TANII Ichiro  Miyazaki Medical College, Anatomy, Instructor, 医学部, 助手 (40207171)
YOSHINAGA Kazuya  Miyazaki Medical College, Anatomy, Associate Proffesor, 医学部, 助教授 (50136719)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordstestis / germ cells / sperm / differentiation / epididymis / fertilization / acrosome reaction / sperm antigen / 抗原 / 単クローン抗体 / 精子形成 / 精子成熟
Research Abstract

[Transport of acrosome materials] The fonnation of the acrosome was hamperd when the vesicle transport inhibitor, breferdin, was added to the culture medium (Tanji et al. (1998) J Electron Microsc, 47 ; 161). [Modification of acrin 1 (MN7=90K) during epididymal maturation] Acrin 1 changed its localization accompanying the morphological change during epididymal maturation (Yoshinaga et at. (1998) Cell Tiss Res, 292 ; 427.). [Function of equatorin (MN9=48K)] The equatorin remained at the equatorial segment until sperm reached to the perivitelline space, although some amount of the equatorin was released during the acrosome reaction. Sperm could not penetrate into the egg, and remained in the perivitelline space under in vitro fertilization (IVF) condition in the presence of anti-equatorin antibody (mMN9). The cortical granules could not be released from the egg. Both pronuclear formation and 2-cell formation were inhibited significantly. Thus, the equatorin is found to be an essential molecule for sperm-egg membrane fusion. (Toshimori et al. (1998) Biol Reprod, 59 ; 22.). [Function of acrin 1 and acrin 2 (MC41=165K)] Acrin 1 was required for the acrosome reaction, while acrin 2 was required for a certain process during after acrosome reaction but before penetration through the zona pellucida. (Saxena et al. (1999) J Reprod Fert, in press.) (Function of acrin 3 (MC1O1=155K)] Acrin 3 was suggested to be related to the gamete fusion, but the detail of the mechanism remained unclear. [Cloning of MC31 antigen (26-35K)] MC31 was highly homologous to the CE9 molecule of rat sperm, which is a molecule of immunoglobulin superfamily. [Review] The maturation of sperm antigenic molecules was reviewed. (Toshimori K (1998) Cell Tissue Res, 293 ; 177.).

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Toshimori, K.: "Maturation of mammalian spermatozoa : modifications of the acrosome and plasma membrane leading to fertilization." Cell Tissue Res.293. 177-187 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toshimori, K., Saxena, D.K Tanii, I. and Yoshinaga, K.: "An MN9 antigenic molecule, equrorin, is equired for successful sperm-oocyte fusion in mice." Biol. Reprod. 59. 22-29 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshinaga, K., Tanii, I., Saxena, D.K. and Toshimori,K.: "Immunocytochemical alterations in the intra-acrosomal antigen MN7 during epididymal maturation of guinea pig spermatozoa." Cell Tissue Res.292. 427-433 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanii, I., Yoshinaga, K. and Toshimori, K.: "The effects of brefeldin A on acrosome formation and protein trnasport to the acrosome in organ cultures of rat seminiferous tubules." J.Electron Microse.47. 161-167 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakai, M., Toshimori, K., Yoshinaga, K., Nasu, T., and Hess, R.A.: "Spermatids of prepubertal rats susceptible to carbendazim-induced testicular toxicity during early spermiogenesis." Arch. Histol.Cytol.61. 433-437 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakai, M., Tishimori, K., Yoshinaga, K., Nasu, T., and Hess, R.A.: "Carbendazim-induced abnormal development of acrosome during early phases of spermiogenesis in the rat testis." Cell Tissue Res.249. 145-152 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 年森清隆: "Modern Reproductive Medicine" メジカルビュー社, 186 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanii I., Yoshinaga, K.and Toshimori K.: "The effects of brefeldin A on acrosome formation and protein trasnport to the acrosome in organ cultures of rat seminiferous tubules." J Electron Microsc. 47. 161-167 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshinaga K., Tanii I., Saxena D.K., and Toshimori K.: "Immunocytochemical alterations in the intra-acrosomal antigen MN7 during epididymal maturation of guinea pig spermatozoa." Cell Tissue Res.292. 427-433 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toshimori K., Saxena D.K., Tanii I.and Yoshinaga K.: "An MN9 antigenic molecule, equatorin, is required for successful sperm-oocyte fusion in mice." Biol Reprod. 59. 22-29 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Saxena D.K., Tanii, I., Yoshinaga K., and Toshimori K.: "Anti-acrosomal monoclonal antibodies, mMN7 and mMC41, inhibit sperm penetration through the zona pellucida in vitro." J Reprod Fert. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toshimori K.: "Maturation of mammalian spermatozoa : modifications of the acrosome and plasma membrane leading to fertilization" Cell Tissue Res. 293. 177-187 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakai M., Toshimori K., Yoshinaga K., Nasu T., and Hess R.A.: "Spermatids of prepubertal male rats are susceptible to carbendazim-induced testicular toxicity during early spermiogenesis." Arch Histol Cytol. 61. 433-437 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakai M., Toshimori K., Yoshinaga K., Nasu T., and Hess R.A.: "Carbendazim-induced abnomal development of acrosome during early phases of spermiogenesis in the rat testis." Cell Tissue Res. 294. 145-152 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toshimori K.: Modern Reproductive Medicine, Vol 1 Egg and Sperm, Role of sperm centriole/centrosome during fertilization (in Japanese). Medical View Press, 68-75 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toshimori K.: Fertilization (in Japanese). Seitai no Kagaku, 48, 219-227 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toshimori,K.: "Maturation of mammalian spermatozoa : modifications of the acrosome and plasma membrane leading to fertilization." Cell Tissue Res.293. 177-187 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toshimori,K.,Saxena,D.K.Tanii,I.and Yoshinaga,K.: "An MN9 antigenic molecule,equatorin,is required for successful sperm-oocyte fusion in mice." Biol.Reprod. 59. 22-29 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshinaga,K.,Tanii,I.,Saxena,D.K.and Toshimori,K.: "Immunocytochemical alterations in the intra-acrosomal antigen MN7 during epididymal maturation of guinea pig spermatozoa." Cell Tissue Res.292. 427-433 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tanii,I.,Yoshinaga,K.and Toshimori,K.: "The effects of brefeldin A on acrosome formation and protein transport to the acrosome in organ cultures of rat seminiferous tubules." J.Electron Microsc.47. 161-167 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakai,M.,Toshimori,K.,Yoshinaga,K.,Nasu,T.,and Hess,R.A.: "Spermatids of prepubertal male rats are susceptible to carbendazim-induced testicular toxicity during early spermiogenesis." Arch.Histol.Cytol.61. 433-437 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakai,M.,Toshimori,K.,Yoshinaga,K.,Nasu,T.,and Hess,R.A.: "Carbendazim-induced abnormal development of acrosome during early phases of spermiogenesis in the rat testis." Cell Tissue Res.294. 145-152 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 年森清隆: "Modern Reproductive Medicine" メジカルビュー社, 186 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 年森 清隆: "受精" 生体の科学. 48. 219-227 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Toshimori, K: "Maturation of mammalian spermatozoa:modifications of the acrosome and plasma membrane leading to fertilization." Cell Tissue Res.accepted. (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Toshimori, K, Saxena, D. K. Tanii, I. and Yoshinaga, K.: "An MN9 antigenic molecule, equatorin, is required for successfur sperm-oocyte fusion in mice." Biol. Reprod. accepted. (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshinaga, K. Tanii, I. Saxena, D. K. and Toshimori, K.: "Immunocytochemical alterations in the intra-acrosomal antigen MN7 during epididymal maturation of guinea pig spermatozoa." Cell Tissue Res.accepted. (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tanii, I.,Yoshinaga, K. and Toshimori, K.: "The effects of brefeldin A on acrosome formation and protein transport to the acrosome in organ cultures of rat seminiferous tudules." J. Electron Mictosc.accepted. (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 年森清隆: "標準細胞生物学" 医学書院, (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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