| Project/Area Number |
09670023
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Iwate Medical University |
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Principal Investigator |
AIZAWA Yukio School of Medicine, Department of Anatomy I, Iwate Medical University, Lecturer, 医学部, 講師 (90095258)
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| Co-Investigator(Kenkyū-buntansha) |
ISOGAI Sumio School of Medicine, Department of Anatomy I, Iwate Medical University, Lecturer, 医学部, 講師 (60212966)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
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| Keywords | angiogenesis of fare-limb / primitive subclaviens / intersegmental arteries / rat embryos / axillarv arterial plexus / brachial plexus / intra-vascular dye-inJection / whole-mounted imuunochemical nerve stain / angiogenesis / limb artery / intersegmental art / rat embryo / brachial plexus / limb bud / Dye-injection / whole-mount stain / intersegmental artery / wholemount staining / Angiogenesis / limb Artery / intersegnseutal Artery / rat emoys / orachial plexus / limb band / whole mount stain |
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| Research Abstract |
It has been believed that the primary arterial trunk of the mammalian forelimb is derived from the 7th intersegmental artery. We examined the early angiogenesis and nerves in the forelimb region by adopting a method that combined intravascular-dye-injection with nerve staining to whole-mounted rat embryos.
The study was carried out on greater numbers of specimens at smaller intervals of embryonic stages and from earlier stages than those in previous reports. We obtained the following results: (1) The multiple primary arterial trunks in the forelimb region (primary subclavians) originate directly from the lateral surface of the dorsal aorta independently of the intersegmental arteries previous to the formation of limb buds. (2) The tips of the 8th (and the 9th) primary subclavians that originate from the aorta near the origin of the 8th (or the 9th) intersegmental artery bend cranially and/or caudally. With the formation of limb bud, they extend to form the longitudinal trunks in the presumptive axillary region. The primary arteries in the free arm region branch off from this longitudinal trunk, and one of them develops into the axial artery. (3) The origins of the primary subclavians shift their positions on the surface of the dorsal aorta and approach the origins of the neighboring intersegmental arteries to join them, and then replace the latter. Consequently. The primary subclavians appear to be "the lateral branches of the intersegmental arteries." (4) The 8th primary subclavian is dominant at first, but is replaced by the 7th primary subclavian which develops into the definitive subclavian artery. (5) With the brachial plexus formation, the axillary arterial plexus derived from the longitudinal trunk develops to form two stems of the axillary artery.
Then we examined the changing process of the axillary arterial plexus by the resin-cast method, the results of which will be soon published.
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