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Importance of carbohydrates in platelets-mediated cell adhesion

Research Project

Project/Area Number 09670028
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKyorin University

Principal Investigator

KAWAKAMI Hayato  Department of Anatomy, Kyorin University School of Medicine, Professor, 医学部, 教授 (30146542)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordscell adhesion / platelet / carbohydrate / tumor cells / metastasis / lectin / endothelium / histochemistry / 大腸癌 / インテグリン / 大腸癌細胞
Research Abstract

Mouse colon carcinoma (colon 26) cells were shown to attach to cultured hepatic sinusoidal endothelial cells only in the presence of PRP (platelet-rich-plasma). Most of tumor cells firstly attached to the endothelial cells not directly but via aggregated platelets. In addition, almost all of the tumor cells that were attached to the endothelial cells showed positive staining with ECA (Erythrina crystagalli agglutinin), whereas nearly half of the cells before adhesion were negative for ECA binding. ECA-positive cells, when separated from ECA-negative ones, revealed strong potency for metastasis to the liver compared with ECA-negative ones. ECA-positive materials were extracted from the crude membrane fraction of colon 26 cells and were found to suppress the platelet adhesion. These results indicate that the ECA-positive sugar chains on the surface of colon 26 cells should mediate the binding between colon 26 cells and platelets and the attachment of tumor cells to the endothelium. The attachment of colon carcinoma cells to hepatic sinusoidal cells induced as above may cause the successive events in establishing cancer metastasis.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Kawakami, H: "Importance of carbohydrates and platelets in the early events of cancer metastasis"Acta Histochem. Cytochem.. 32. 203-208 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamamoto, K: "Immunohystochemical localization of β1,4-galactosyltransferase in human pancreatic tissues"Pancreas. 19. 137-142 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fukawa, W: "Changes in lection binding pattern in rat esophageal epithelium during development"Okajimas Folia Anat Jpn. 75. 335-344 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kobayashi, Y: "Binding of Griffonia simplicifolia I-B_4 lection in the substantia gelationosa of rat spinal cord"Acta Histochem. Cytochem.. 31. 509-515 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 川上速人: "血小板の電子顕微鏡像-活性化と突起形成"Medico. 29. 12870-12872 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山口嘉和: "胃癌とレクチン"Medio. 15. 111-113 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamaguchi H, Osaki T, Taguchi H, Yamamoto T, Hanawa T, Fukuda M, Kawakami H, Hirano H & Kamiya S: "Growth inhibition of Helicobacter pylori by monoclonal antibody to heat-shock protein 60"Microbiol Immunol. 41. 909-916 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Osaki T, Yamaguchi H, Taguchi H, Fukuda M, Kawakami H, Hirano H, Watanabe S, Takagi A, Kamiya S: "Establishment and characterisation of monoclonal antibody to inhibit adhesion of Helicobacter phlori to gastric epithelial cells"J Med Microbiology. 47. 505-512 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kurosaki M, Watanabe T, Hori T, Hirai K, Kawakami H, Hirano H: "Co-localization of human growth hormone and human prolactin in hormonally pure human growth hormone-producing pituitary adenomas"Neurologia Medico-Chirurgica. 38. 335-341 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kobayashi Y, Kawakami H, Yoshino Y, Hirano H: "Binding of Griffonia simplicifolia I-B4 lectin in the substantia gelatinosa of the rat spinal cord"Acta Histochem. Cytochem.. 31. 509-515 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fukawa W, Kawakami H, Hirano H: "Changes in lectin binding pattern in rat esophageal epithelium during development"Okajimas Folia Anat. Jpn.. 75. 335-344 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kawakami H, Hirano H: "Importance of carbohydrates and platelets in the early events of cancer metastasis"Acta Histochem. Cytochem.. 32. 203-208 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hanawa T, Fukuda M, Kawakami H, Hirano H, Kamiya S, Yamamoto T: "The Listeria monocytogenes DnaK chaperone is required for stress tolerance and efficient phagocytosis with macrophages"Cell Stress & Chaperones. 4. 118-128 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamamoto K, Kawakami H, Sugiyama M, Atomi Y, Hirano H: "Immunohistochemical localization of β-1, 4-galactosyltransferase in human pancreatic tissues"Pancreas. 19. 137-142 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kamiya S, Yamaguchi H, Osaki T, Taguchi H, Fukuda M, Kawakami H, Hirano H: "Effect of an aluminum hydroxide-magnesium hydroxide combination drug on adhesion, IL-8 inducibility, and expression of HSP60 by Helicobacter pylori"Scand. J. Gastroenterol.. 34. 663-670 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kawakami H et al: "Importance of carbolydrates and platelets in the early events of cancer metastasis" Acta Histochem.Cytorhem. 32(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamamoto K.et al.: "Immunohistockemical localization of β1,4-glactosyltransferare in human pancreas" Pancreas. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kobayashi Y.et al.: "Binding of Griffonia simplicifolia I-B_4 Lectin in the sbstantia gelatinosa of the rat spinal cord" Acta Histochem.Cytochem. 31. 509-515 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kurosaki M.et al.: "Co-localization of human growth hormore and huneniprolactin in hermoralhypure human growth hormore-produing pitritary aderoms" Neurol.Medicochirurgica. 38. 335-341 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 川上速人 他: "血小板の電子顕微鏡像-活性化と突起形成" Medico. 29. 12870-12872 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 松田 実: "乳癌組織におけるHSP90・pS2とホルモンレセプターの免疫組織化学的研究" 乳癌基礎研究. 6. 24-28 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamaguchi,H.: "Grouth inhibition of Helicobacter pylori by maralinal antibody to heat-shack protein 60" Microbiol Immunol. 41(in press). (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Osaki T.: "Estabishment and cherocterizarion of monoclonal antibody to inhibit celhesion of Helicobacter pylori to gastric epithelial cells." J Med Microbiology. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 川上速人: "血小板の電子顕微鏡像-活性化と突起形成" MEDICO. 29(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] KAWAKAMI,H.: "Changes in lectin binding pattern of Leydig cells cluring fetal and postnatel development in mice.「笹川医学奨学金十周年記念論文集」" 中華人民共和国衛生部, 7 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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