• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Aminergic and peptidergic innervation of neuroendocrine cells and its signal transduction system

Research Project

Project/Area Number 09670029
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionShowa University

Principal Investigator

NAKAI Yasumitsu  Showa University, School of Medicine, Professor, 医学部, 教授 (60053807)

Co-Investigator(Kenkyū-buntansha) SHIODA Seiji  Showa University, School of Medicine, Associate Professor, 医学部, 助教授 (80102375)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsPACAP / Noradrenalin / Vasopressin / Siganal transduction / Calcium channel / Neuroendocrine cells / バイプレシン / 神経内分泌細胞 / 神経支配 / アミン / レセプター / 免疫組織化学 / in situハイブリダイゼーション / 細胞内伝達機構
Research Abstract

Although the pathway arising from PACAP (Pituitary Adenylate Cydase-Activating Polypeptide) INA (Noradrenalin)-containing neurons in the ventrolateral medulla is invoked in the regulation of arginine vasopressin (AVP), the precise mechanism of action and physiological significance of the coexistence of PACAP and NA still need to be investigated. Both PACAP and NA induced large increases in the [Ca^<2+>]i in isolated AVP-containing neurons. When PACAP and NA were added together, they evoked larger increase in [Ca^<2+>]i in AVP neurons. An inhibitor of PKA (protein kinase A) completely inhibited the PACAP-induced increase in [Ca^<2+>]i, and partly the NA-induced increase in AVP-containing neurons. We found that T-type calcium channels might contribute to Ca^<2+> influx during action potentials through cAMP-PKA signaling pathway and L-type currents might contribute to the generation of bursting activity. PACAP and NA, co-released from the same axon terminals, may act synergistically to stimulate calcium signaling in AVP neurons, which is mediated by both cAMP-PKA pathway and L-type Ca^<2+> channel. It is hypothesized that PACAP/NA regulate the functions of AVP-containing neurons which participate in the control of plasma osmolarity and blood pressure.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] 塩田清二: "視床下部-下垂体系の構造" CLINICAL NEUROSCIENCE. 15. 1212-1214 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shioda, S.: "Pituitary adenylate cyclase-activating polypeptide(PACAP) : a novel regulator of vasopressin - containing neurons." Brain Res.765. 81-90 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shioda, S.: "Noradrenaline activates vasopressin neurons via α_1-receptor- mediated Ca^<2+> signaling pathway." Neurosci. Lett.226. 210-212 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shioda, S.: "Neuronal nicotinic acetylcoline receptor in the hypothalamus : morphological diversity and neuroendocrine regulations." Mol.Brain Res.47. 45-54 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shioda, S.: "Nicotine increases cytosolic Ca^<2+> in vasopressin neurons." Neurosci.Res.29. 311-318 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shioda, S.: "Functional significance of medullary noradrenergic innervation of rat hypothamic vasopressin neurons." Advances in Comparative Endocrinology. 1. 677-680 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shioda, S.: "PACAP increases cytosolic calcium in vasopressin neurons : synergism with noradrenaline." Ann.NY.Acad.Sci.865. 427-430 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shioda, S.: "Vasopressin neuron activation and Fos expression by stimulation of the caudal ventrolateral medulla." Brain Res.Bull.45. 443-450 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shioda, S., Nakai, Y: CLINICAL NEUROSCIENCE. Vol.15 No.11. 1212-1214 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shioda, S., Yada, T., Nakajo, S., Nakaya, K., Nakai, Y.and Arimura, A.: "Pituitary adenylate cyclase-activating polypeptide (PACAP) : a novel regulator of vasopressin-containing neurons." Brain Res.765. 81-90 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shioda, S., Yada, T., Muroya, S., Takigawa, M.and Nakai, Y.: "Noradrenaline activates vasopressin neurons via alpha_1-receptor-mediated Ca^<2+> signaling pathway." Neurosci.Lett.226. 210-212 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shioda, S., Nakajo, S., Hirabayashi, T., Nakayama, H., Nakaya, K., Matsuda, K.and Nakai, Y.: "Neuronal nicotinic acetylcholine receptor in the hypothalamus : morphological diversity and neuroendocrine regulations." Mol.Brain Res.47. 45-54 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shioda, S., Yada, T., Muroya, S., Takigawa, M.and Nakai, Y.: "Nicotine increases cytosolic Ca^<2+> in vasopressin neurons." Neurosci.Res.29. 311-318 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shioda, S.and Nakai, Y.: "Functional significance of medullary noradrenergic innervation of rat hypothalamic vasopressin neurons." Advances in Comparative Endocrinology 1,677-680,1997 Monduzzi Editore (Bologna).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shioda, S., Yada, T., Nakajo., S., Nakai, Y.and Arimura, A.: "PACAP increases cytosolic calcium in vasopressin neurons : synergism with noradrenaline." Ann.NY.Acad.Sci.865. 427-430 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shioda, S., Iwase, M., Homma, I., Nakajo, S., Nakaya, K.and Nakai, Y.: "Vasopressin neuron activation and Fos expression by stimulation of the caudal ventrolateral medulla." Brain Res.Bull.45. 443-450 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 塩田清二: "視床下部-下垂体系の構造" CLINICAL NEUROSCIENCE. 15. 1212-1214 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shioda,S.: "Pituitary adenylate cyclase-activating polypeptide(PACAP):a novel regulator of vasopressin-containing neurons." Brain Res.765. 81-90 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shioda,S.: "Noradrenaline activates vasopressin neurons via α_1-receptor-mediated Ca^<2+> signaling pathway." Neurosci.Lett.226. 210-212 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shioda,S.: "Neuronal nicotinic acetylcholine receptor in the hypothalamus:morphological deversity and neuroendocring regulations." Mol.Brain Res.47. 45-54 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shioda,S.: "Nicotine increases cytosolic Ca^<2+> in vasopressin neurons" Neurosci.Res.29. 311-318 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shioda,S.: "Functional signifiuance of medullary noradrenergic innervation of rat hypothalamic vasopressin neurons." Advances in Comparative Endocrinology.1. 677-680 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shioda,S.: "PACAP in creases cytosolic calcium in vasopressin neurons:synergism with noradrenaline." Ann.NY.Acad.Sci.865. 427-430 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shioda,S.: "Vasopressin neuron activation and Fos expression by stimulation of the caudal ventrolateral medulla." Brain Res.Bull.45. 443-450 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shioda S: "Pituitary adenylate cyclase-activating polypeptide(PACAP): a novelregulator of vasopressincontaining neurons." Brain Res. 765. 81-90 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Li M: "Specific antibody recognition of rat pituitary adenylate cyclase activating polypeptide receptors." Endocrine. 7. 183-190 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shioda S: "Localization and gene expression of the receptor for pituitary adenylate cyclase-activating polypeptide in the ratbrain." Neurosci Res. 28. 345-354 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shioda S: "Noradrenaline activates vasopressin neurons via α1-receptor mediated Ca^<2+>signaling pathway." Neurosci Lett. 226. 210-212 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shioda S: "Neuronal nictotinic acetylcholine receptor in the hypothalamus : morphological diversity and neuroendocrine regulations." Mol Brain Res. 49. 45-54 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Seki T: "Distribution and ultrastructurall ocalization of a receptor for pituitary adenylate cyclase activating polypeptide and its mRNA in the ratretina." Neurosci Lett. 238. 1-4 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shioda S: "Nicotine increases cytosolic Ca^<2+> in vasopressin neurons." Neurosci Res. 29. 311-318 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shioda S: "Pituitary adenylate cyclase-activating polypeptide : a key player in male reproduction?" Ann NY Acad Sci. 839. 524-525 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shioda S: "Vasopressin neuron activation and Fos expression by stimulation of catecholaminergic neuorns in the caudal ventrolateral medulla." Brain Res Bull. 45(in press). (1998)

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi