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Immunocytochemistry and in situ hybridization of some vasoactive peptides and their receptors

Research Project

Project/Area Number 09670035
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionUniversity of Occupational and Environmental Health

Principal Investigator

FUJIMOTO Sunao  Univ.Occup.Environ.Health, Sch.Med, Dept.of Anatomy, Professor, 医学部, 教授 (80080547)

Co-Investigator(Kenkyū-buntansha) KUDO Hideaki  Univ.Occup.Environ.Health, Sch.Med, Dept.of Anatomy, Assistant Professor, 医学部, 講師 (40289575)
DOI Yoshiaki  Uni.Occup.Environ.Health, Sch.Med, Dept.of Anatomy, Associate Professor, 医学部, 助教授 (30258602)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1998: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsendothelial cell / endothelin-1 / calcitonin gene-related peptide / hypoxia / in situ hybridization / Weibel-Palade body / immunocytochemistry / endothelin receptor / カルシトニン遺伝子関連ペプチド / ワイベル・パラーテ小体 / ワイベル・パラ-デ小体
Research Abstract

1)The rat carotid body artery (CBA) and thoracic aorta under the hypoxic condition were investigated by electron microscopy, immunocytochernistry and in-situ hybridization. A remarkable increase in the number of Weibel-Palade (WP) bodies occurred in endothelial cells of the hypoxic CBA and thoracic aorta. The degree of immunoreactivities and gene expressions of both endothelin (ET)-1 and calcitonin gene-related peptide (CGRP) was significantly enhanced in the hypoxic samples when compared to the normoxic ones. Since WP bodies are involved in the storage and release of these peptides, the present findings indicate that ET-1 and CGRP, which are actively synthesized and released from endothelial cells, play a crucial role in the regulation of the blood flow through these vessels in the hypoxic condition.
2)Vasoactivities after treatment with ET-1, and immunoreactivities for ET receptors were investigated on the rat superior mesenteric vasculature (SMV). By the me asurement of corrosion cast images of the SMVs, it was seen that ET-1. induces the remarkable vasocontraction of the distal arterial branches. The present immunocytochemistry showed that immunoreactivities for ETA receptors are localized to the sarcolemma of medial muscle, cells of the distal arterial branches. These findings suggest that ET-1-induced vasocontraction on the distal arterial branches is mediated from the ETA receptors. Immunoreactivities for ETB receptors were seen on the plasmalemma of endothelial cells in the SMVs. Since the WP bodies of these endothelial cells showed ultrastructural changes such as degranulation and exocytosis after treatment with sarafotoxin S6c, ETB receptor selective agonist, it seems likely that ETB receptors of the SMVs are involved in the release of the WP bodies.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Kotaro Kayashima 他: "The role of endothelin-1 in regulation of rat mesenteric microcirculation" Journal of Cardiovascular Pharmacology. 31(Suppl.1). S126-S127 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomohiko Nomiyama 他: "Immunocytochemistry of fibronectin and endothelin-1 in the cavernous body of postnatal rabbit penises" Acta anatomica. 162. 23-32 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takeyuki Fujimura 他: "Immunoreactivity of endothelins and endothelin receptor in the stria vascularis of the mouse cochlea" Hearing Research. in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kotaro Kayashima 他: "Effects of endothelin-1 on vasoactivity and its synthesis, storage, and acting sites in the rat superior mesenteric vasculature : an ultrastructural and immunocytochemical study" Medical Electron Microscopy. in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takatoshi Ozaka 他: "18th UOEH International Symposium" Elsevier Science(in press),

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kotaro Kayashima et al.: "The role of endothelin-1 in requlation of rat mesenteric microcirculation" Journal of Cardiovascular Pharmacology. 31(Suppl.1). S126-S127 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomohiko Nomiyama et al.: "Immunocytochemistry of fibronectin and endothelin-1 in the cavernous body of postnatal rabbit penisea" Acta Anatomica. 162. 23-32 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takeyuki Fujimura et al.: "Immunoreactivity of endothelins and endothelin receptor in the stria vascularis of the mouse cochles" Hearing Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kotaro Kayashima et al.: "Effects of endothelin-1 on vasoactivity and its synthesis, storage, and acting sites in the rat superior mesenteric vasculature : an ultrastructural and immunocytochemical study" Medical Electron Microscopy. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takatoshi Ozaka et al.: "Enhancement in the synthesis and release of calcitonin gene-related peptide and endothelin-1 in the hypoxic rat carotid body artery" 18th UOEH International Symposium. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomohiko Nomiyama 他: "Immunocytochemistry of fibronectin and endothelin-1 in the cavernouse body of postnatal rabbit penises" Acta Anatomica. 162. 23-32 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takeyuki Fujimura 他: "Immunoreactivity of endothelins and endothelin receptor in the stria vascularis of the mouse cochlea" Hearing Research. in press.

    • Related Report
      1998 Annual Research Report
  • [Publications] Kotaro Kayashima 他: "Effects of endothelin-1 on vasoactivity and its synthesis,storage, and acting sites in the rat superior mesenteric vasculature: an ultrastructural and immunocytochemical study." Medical Electron Microscopy. in press.

    • Related Report
      1998 Annual Research Report
  • [Publications] Takatoshi Ozaka 他: "18th UOEH International Symposium" Elsevier Science(in press),

    • Related Report
      1998 Annual Research Report
  • [Publications] Takatoshi Ozaka etal.: "Weibel-Palade bodies as a storage site of calcitonin gene-related peptide and endothelin-1 in blood vessels of the rat carotid body" Anatomical Record. 247・3. 388-394 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kotaro Kayashima etal.: "The Role of Endothelin-1 in Regulation of Rat Mesenteric Microcirculation" Journal of Cardiovascular Pharmacology. 31.(Suppl.1). S126-S127 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 土肥 良秋: "内皮由来の血管作動性因子" 電子顕微鏡. (in press).

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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