• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Structural determination of agonist binding site of ADPbS-sensitive P2

Research Project

Project/Area Number 09670057
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionNIHON UNIVERSITY

Principal Investigator

KOKUBUN Shinichiro  Nihon Univ. Sch. Of Med., Professor, 医学部, 教授 (20153520)

Co-Investigator(Kenkyū-buntansha) TAKAO Kyoichi  Nihon Univ. Sch. Of Med., Assistant, 医学部, 助手 (90187922)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsP2X1 / P2Y2 / P2Y4 / Urinary bladder / RT-PCR / ntracellular Calcium / ラット / 膀胱平滑筋細胞 / ヌクレオチド受容体 / クローニング / 細胞内Ca / Polymerase Chain Reaction
Research Abstract

We investigated subtypes of P2-purinoceptors in rat urinary bladder smooth muscle (UBSM) cells. In the experiments using isolated single UBSM cells, in the presence of extracellular 1.8 mM Ca2+ (10ィイD1-8ィエD1 M to 10ィイD1-4ィエD1 M) and P2X1 agonist, α,β-methylene adenosine 5'-triphosphate (α,β-Me ATP) (10ィイD1-9ィエD1 M to 10ィイD1-4ィエD1 M), increased intracellular CaィイD12+ィエD1 ([CaィイD12+ィエD1]ィイD2iィエD2) monitored with 1-[2-(5-carboxyoxazol-2-yl)-6-aminobenz-furan-5-oxy]-2-(a-amino-5-methylphenoxy)-ethane-N,N,N,N-tetraacetic acid (fura-2). In the absence of extracellular CaィイD12+ィエD1, α,β-Me ATP (10ィイD1-9ィエD1 M to 10ィイD1-4ィエD1 M) didnot increase [CaィイD12+ィエD1]ィイD2iィエD2, whereas ATP (10ィイD1-8ィエD1 M to 10ィイD1-4ィエD1 M) increased it. The potency order of P2Y-purinoceptor agonists in the increase in [CaィイD12+ィエD1]ィイD2iィエD2 in the absence of extracellular CaィイD12+ィエD1 was ATP>UTP>ADP>UDP. Reverse transcription polymerase chain reaction (RT-PCR) showed P2X1, P2Y2, P2Y4 and P2Y1 transcripts in these cells, and the relative quantity of mRNAs determined by ABI PRISM 7700 was P2X1>>P2Y2>>P2Y4>>>>P2Y1. These results suggest that the major purinoceptors expressed in rat UBSM cells are P2X1 and P2Y2.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Masahiro NARAMTSU: "The signalling pathway which causes contraction via P_2-purinoceptors in rat urinary bladder smooth muscle"British Jounal of Pharmacology. 122. 558-562 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 國分眞一朗: "ラット膀胱平滑筋におけるP_2受容体を介する収縮の情報伝達路"日本平滑筋学会誌(Japan Section). 1・2. J-81 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 楢松雅裕: "P_2プリン受容体を介するラット膀胱平滑筋の収縮に関与する細胞内情報伝達系の解析"日大医学雑誌. 57(5). 263 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Masahiro Naramatsu and Shiniohiro Kokubun: "The signalling pathway which causes contraction via P2-purinoceptors in rat urinary bladder smooth muscle"Br. J. Pharmacol. 122. 5587-562 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 楢松 雅裕: "P_2プリン受容体を介するラット膀胱平滑筋の収縮に関与する細胞内情報伝達系の解析" 日本医学雑誌. 57(5). 263 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Masahiro NARAMATSU: "The signalling pathway which causes contraction via P_2-purinoceptors in rat urinary bladder smooth muscle" British Journal of Pharmacology. 122. 558-562 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 國分 眞一朗: "ラット膀胱平滑筋におけるP_2受容体を介する収縮の情報伝達路" 日本平滑筋学会誌(Japan Section). 1・2. J-81 (1997)

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi