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Analysis of angiogenesis in fibrinolytic factordefficient mice

Research Project

Project/Area Number 09670062
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionDepartment of Physiology, Kinki University School of Medicine

Principal Investigator

MATSUO Osamu  Department of Physiology, Kinki University School of Medicine, Professor, 医学部, 教授 (40030879)

Co-Investigator(Kenkyū-buntansha) OKADA Kiyotaka  Department of Physiology, Kinki University School of Medicine, Assistant, 医学部, 助手 (20185432)
FUKAO Hideharu  Department of Physiology, Kinki University School of Medicine, Assistant, 医学部, 助手 (70218874)
UESHIMA Shigeru  Department of Physiology, Kinki University School of Medicine, Assistant Profess, 医学部, 助教授 (30193791)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsFibrinolysis / t-PA / u-PA / PAl-1 / PLGF / Capsule / Knock out mouse / Fibrin / Angiogenesis / Endothelial cells
Research Abstract

The mouse lines which lack the genes for placenta growth factor(PLGF), urokinase-type plasminogen activator (u-PA), tissue-type plasminogen activator (t-PA) and type 1 plasminogen activator inhibitor (PAl-1 ), respectively were established. The capsule-implantation method was useful for the analysis of wound healing process in these mice, since the formation of granulation tissues and tissue fluids were observed to be increased in a time dependent manner after the implantation of capsules. This model demonstrated that the lack of PLGF delayed the formation of granulation tissues inside the capsule at an early stage of wound healing compared with that of wild-type mouse. However, it was considerably improved in the presence of fibrin in the capsule, suggesting that fibrin acted as a good matrix for inducing tissue repair. This method was also applicable in the fibrinolytic factor-diffecient mice. The effect of fibrin in the capsule was also found to be effective for the regeneration of granulation tissues in ery fibrinolytic factor-diffecient mouse. However, there was no difference in the rate of tissue repair among these mice. Neither activities of matrix metalloproteinases in the intracapsular fluids and intracapsular tissue extracts, nor the proliferation of cultured endothelial cells were not influenced by the defficiency of these fibrinolytic factors. Therefore, the effect of u-PA, t-PA and PAl-1 on the process of angiogenesis was not evident by the capsule-implantation model.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (1 results)

All Other

All Publications (1 results)

  • [Publications] Fukao, H., Hagiya, Y., Ueshima, S., Kamiishi, H., Okada, K., and Matsuo, O.: "Fibrinolytic system in rat angiogenesis" International Journal of Angiology. (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary

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Published: 1997-04-01   Modified: 2016-04-21  

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