| Project/Area Number |
09670081
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Hyogo College of Medicine |
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Principal Investigator |
NAKAGAKI Ikuko Hyogo College of Medicine, Medicine, Assistant Professor, 医学部, 講師 (90084904)
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| Co-Investigator(Kenkyū-buntansha) |
HORI Kazuko Hyogo College of Medicine, Medicine, Assistant Professor, 医学部, 講師 (30068514)
TSUJITA Jyunzo Hyogo College of Medicine, Medicine, Assistant Professor, 医学部, 講師 (30104235)
SASAKI Sadao Hyogo College of Medicine, Medicine, Associate professor, 医学部, 助教授 (20104276)
HORI Seiki Hyogo College of Medicine, Medicine, Professor, 医学部, 教授 (80068452)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | brown adipose tissue / cold acclimation / Ca^<2+> / heat production / ion / CLSM / mitochondria / 寒冷曝露 / 熱産生 |
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| Research Abstract |
The changes of ion concentrations such as Ca, Na, K and Cl in warm (WA) and cold (CA) acclimated brown adipose cells were investigated.
Morphometric analyses of brown adipocytes showed that lipid droplets occupied 75% of the WA cells and 37% of the CA cells. The area of a single mitochondrion in CA cells was three times that of one in the WA cells.
The fluorescence intensity of fluo-3 (Ca^<2+> indicating dye), which was loaded for 30 min, increased within 1 min of noradrenaline (NA) exposure in the cytoplasm of WA and CA cells. In contrast, in the nucleus with NA application fluorescence intensity rose after an initial decrease in WA, and in CA decreased gradually. Acridine orange (AO), a fluorescent dye which can indicatea H^+ gradient, was localized exclusively in the mitochondria rich cytoplasm of CA cells.
X-ray microanalysis showed that Na was higher, and K and Cl were lower in CA than WA, in both the nucleus and the mitochondria. Moreover, Ca (total) was higher in the mitochondria, whereas was lower in the nucleus of CA cells than in WA cells. The patch clamp record shows a channel activity, when the reconstituted mitochondrial membrane has some voltage and pH gradient. The whole cell clamp record from oocyte injected with total RNA of CA shows the voltage dependent outward currents.
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