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MOLECULAR PHARMACOLOGICAL STUDIES ON MECHANISMS ASSOCIATED WITH NEUROMODULATION BY GLUTATHIONE

Research Project

Project/Area Number 09670111
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General pharmacology
Research InstitutionSETSUNAN UNIVERSITY

Principal Investigator

OGITA Kiyokazu  SETSUNAN UNIVERSITY,DEPARTMENT OF PHARMACOLOGY,LECTURER, 薬学部, 講師 (90169219)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsGLUTATHIONE / NEUROMODULATOR / GLUTAMIC ACID / TRANSCRIPTION FACTOR / ACTIVATOR PROTEINN-1 / KINIC ACID / GLUTATHIONE DEPLETOR / GLUTATHIONE BINDING PROTEIN / グルタミン酸レセプター / グルタミン酸取り込み / γ-アミノ酸取り込み / N-メチル-D-アスパラギン酸レセプター / シナプス膜 / 脳
Research Abstract

In vitro addition of glutathione did not significantly affect uptake of [^3H]-glutamic (Glu) and [^3H] gamma-aminobutyrrc (GABA) acids into rat synaptosomal fractions at a concentration range of up to 1 mM These results suggest that glutathione plays no physiological role in transport systems of Glu and GABA into neuronal and/or glial cells. On the other hand, an intraperitoneal injection of kainic acid (KA) at a subconvulsive low dose markedly potentiated DNA binding ac tivity of the transcription factor activator protein-l (APl) in the hippocampus of mice with reduced contents of brain endogenous gluthatione following a previous injection of the glutathione depletor 2-cyclohexen-l-one, without markedly affecting that in animals previously injected with saline alone. However, the depletor did not significantly modulate the potentiation of APl DNA binding by the administration of N-methyl-D-aspartic acid (NMDA) in murine hippocampus. The data cited here raise the possibility that endogenous glutathione may play a critical role in mechanisms underlying signaling cascade through KA, but not NMDA,receptors to nuclear AP1 in murine hippocampus. Moreover, several membrane proteins with different molecular weights were isolated from porcine brains following column chromatography using affinity resins immobilized with glutathione.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Yukio Yoneda: "Prolongation by bifemelane of potentiation of AP1 DNA binding in hippocampal CA1 subfield of gerbils with transient forebrain ischemia." J.Neurosci.Res.51. 574-582 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yasutaka Azuma: "Possible in vivo crosstalk between transcription factors with zinc-finger and leucine-zipper motifs in murine peripheral but not central excitable tissues." Neurochem.Int.32. 325-336 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yukio Yoneda: "Possible involvement of AP1 DNA binding in mechanisms underlying ischemia tolerance in the CA1 subfield of gerbil hippocampus." Neuroscience. 86. 79-97 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kiyokazu Ogita: "Nitric oxide-independent inhibition by sodium nitroprusside of the native N-methyl-D-aspartate recognition domain in a manner different from that by potassium ferrocyanide." Neurochem.Int.in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yukio Yoneda: "Excitatory amino acid signaling to nuclear transcription factors." Critical Reviews in Neurobiology. in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kiyokazu Ogita: "Modulation by sodium nitroprusside of the native N-mathyl-D-aspartate receptor in a manner independent of nitric oxide radical." Current Topics of Pharmacology. in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 荻田喜代一: "グルタミン酸Caイオンチャネル" 日本臨床. 56. 1819-1823 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 荻田喜代一: "グルタミン酸神経作用薬と中枢神経系疾患" 脳21. 1. 183-189 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 倉本展行: "NMDA受容体" 生体の科学. 印刷中.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kiyokazu Ogita: "Glutathione in the Nervous System" Taylor & Francis, 389 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kiyokazu Ogita: "Methods in Enzymology" Academic Press in press,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoneda Y., et al.: "Prolongation by bifemerane of potentiation of AP1 DNA binding in hippo-campal CA1 subfieeld of gerbils with transient forebrain ischemia." J.Neurosci.Res.51. 574-582 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Azuma Y., et al.: "Possible in vivo crosstalk between transcription factors with zinc-finger and leucine-zipper motifs in murine peripheral but not central excitable tissues." Neurochem.Int.32. 325-336 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoneda Y., et al.: "Possible involvement of AP1 DNA binding in mechanisms underlying ischemic tolerance in the CA1 subfield of gerbil hippocampus." Neuroscience. 86. 79-97 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ogita K., et al.: "Nitric Oxide-independent inhibition by sodium nitroprusside of the native N-methyl-D-aspartate recognition domain in a manner different from that by potassium ferrocyanide." Neurochem.Int. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoneda Y., et al.: "Excitatory amino acid signaling to nuclear transcription factors." Critical Rev.Neurobiol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ogita K., et al.: "Modulation by sodium nitroprusside of the native N-methyl-D-aspartate receptor in a manner independent of nitric oxide radical." Current Topics Pharmacol. (in Press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ogita K., et al.: Gluthatione in the Nervous System. Taylor & Francis, (in press), (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ogita K., et al.: Methods in Enzymology. Academic Press, (in press), (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yukio Yoneda: "Prolongation by bifemelane of potentiation of AP1 DNA binding in hippocampal CA1 subfield of gerbils with transient forebrain ischemia." J.Neurosci Res. 51. 574-582 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yasutaka Azuma: "Possible in vivo crosstalk between transcription factore with zinc-finger and leucine-zipper motifs in murine peripheral but not central excitable tissues." Neurochem.Int. 32. 325-336 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yukio Yoneda: "Possible involvement of AP1 DNA binding in mechanisms underlying ischemia tolerance in the GA1 subfield of gerbil hippocampus." Neuroscience. 86. 79-97 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kiyokazu Ogita: "Nitric oxide-independent inhibition by sodium nitroprusside of the native N-methyl-D-aspartate recognition domain in a manner different from that by potassium ferrocyanide." Neurochem.Int.(in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Yukio Yoneda: "Excitatory amino acid signaling to nuclear transcription factors." Critical Reviews in Neurobiology. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Kiyokazu Ogita: "Modulation by sodium nitroprusside of the native N-methyl-D-aspartate receptor in a manner independent of nitric oxide radical." Current Topics of Pharmacology. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Kiyokazu Ogita: "Glutathione in the Nervous System" Taylor & Francis, 389 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kiyokazu Ogita: "Methods,in Enzymology" Academic Press(in press),

    • Related Report
      1998 Annual Research Report
  • [Publications] Kiyokazu Ogita: "Nitric oxide-Independent Inhibition by sodium nitroprusside of the native N-methyl-D-aspartate recognition domain in a manner different from that by potassium femocya" Neurochem.Int.(発表予定). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kiyokazu Ogita: "Glutathione in the Nervous System" Taylor & Francis, 389 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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