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Roles of stress protein hsp105 during mouse embryo development.

Research Project

Project/Area Number 09670139
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionKyoto Pharmaceutical University

Principal Investigator

HATAYAMA Takumi  Kyoto pharmaceutical University, Professor, 薬学部, 教授 (10094484)

Co-Investigator(Kenkyū-buntansha) YAMAGISHI Nobuyuki  Kyoto pharmaceutical University, research associate, 薬学部, 助手 (60298685)
YASUDA Kunihiko  Kyoto pharmaceutical University, research associate, 薬学部, 助手 (50278446)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsStress protein / HSP105 / HSP105 gene / Apoptosis / Embryo developement / Heat shock protein / Mouse
Research Abstract

1. The 105-kDa heat shock protein (HSP105) is a member of the high molecular mass heat shock protein family. To elucidate the genomic structure of mouse HSP105 and to examine the regulation of expression of its gene, we have isolated and characterized the mouse HSP105 gene including about 1.2kb of the 5'-flanking region. (1) The mouse HSP105 gene spans about 22 kb, consisting of 18 exons separated by 17 introns. (2) Southern blotting analysis revealed the existence of a single copy of HSP105. (3) Primer extension analysis revealed that the transcription initiation site was located 165 bp upstream of the ATG translation initiation codon. (4) The 5'-promoter region of the HSP105 gene contained a TATA box, a CAAT box, an inverted CAAT box and two GC boxes. Two heat shock element (HSE) sequences were found as four nGAAn repeats at nt -64 and nt -128. (5) Promoter analysis using deletion derivatives revealed that a minimal region which contained the two consensus HSE sequences was active in response to heat shock and also for constitutive expression of the gene.
2. To examine the role of HSP105 for differentiation and apoptosis.of mouse teratocarcinoma F9 cells, mouse HSP105alpha cDNA expression plasmid constructed with pcDNA3 vector was introduced into F9 cells, and isolated two HSP105alpha-overexpressing cells which expressed HSP105 at 2-3-fold higher levels than parent cells. (2) In response to various stresses such as heat shock, actinomycin D, etoposide and hydrogen peroxide, the HSP105alpha-overexpressing cells were more sensitive than parent cells or the cells transfected with pcDNA3 vector. (3) Since the increased sensitivity to these stresses was due to an increase of apoptotic cell death, HSP105alpha was suggested to enhance apoptosis. Experiments to elucidate precise mechanisms of HSP105alpha for apoptosis are now in progress.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Takumi Hatayama: "Characteristic expression of high molecular mass heat shock protein HSP105 during mouse embryo development." Cell Struct.Funct.22. 517-525 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takumi Hatayama: "Reduced induction of HSP70 in PC12 cells during neuronal differentiation." J.Biochem.122. 904-910 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takumi Hatayama: "Effects of hyperasmotic NaCl and glycerol stress on stress response of human HeLa cells." Biol.Pharm.Bull.20. 605-612 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hiroshi Oshima: "A possibility for new evaluating method of cytotoxicity by using heat shock protein assay." J.Mater.Sci. : Mater.Med.8. 143-147 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takumi Hatayama: "Association of HSP105 with HSC70 in high molecular mass complexes in mouse FM3A cells." Biochem.Biophys.Res.Commun.248. 395-401 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tohru Wakatsuki: "Characteristic expression of 105-kDa heat shock protein (HSP105) in various tissues of nonstressed and heat-stressed rats." Biol.Pharm.Bull.21. 905-910 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Keiichi Ishihara: "Molecular cloning, expression and localization of human 105 kDa heat shock protein, hsp105." Biochim.Biophys.Acta. 1444. 138-142 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kunihiko Yasuda: "Genomic cloning and promoter analysis of mouse 105-kDa heat shock protein (hsp105) gene." Biochem.Biophys.Res.Commun.印刷中. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takumi Hatayama, Toshiya Takigawa, Sadao Takeuchi, and Kohei Shiota: "Characteristic expression of high molecular mass heat shock protein HSP105 during mouse embryo development." Cell Struct.Funct.22(5). 517-525 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takumi Hatayama, Hisaki Takahashi, and Nobuyuki Yamagishi: "Reduced induction of HSP70 in PC12 cells during neuronal differentiation." J.Biochem.122(5). 904-910 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takumi Hatayama, Souichi Fujimoto, and Kayo Sakai: "Effects of hyperosmotic NaCl and glycerol stress on stress response of human HeLa cells." Biol.Pharm.Bull.20(6). 605-612 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hiroshi Oshima, Takumi Hatayama, and Masaaki Nakamura: "A possibility for new evaluating method of cytotoxicity by using heat shock protein assay." J.Mater.Sci. : Mater.Med.8(3). 143-147 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takumi Hatayama, Kiyoko Yasuda, and Kunihiko Yasuda: "Association of HSP105 with HSC70 in high molecular mass complexes in mouse FM3A cells." Biochem.Biophys. Res.Commun.248(2). 395-401 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tohru Wakatsuki and Takumi Hatayama: "Characteristic expression of 105-kDa heat shock protein (HSP105) in various tissues of nonstressed and heat-stressed rats." Biol.Pharm.Bull.21(9). 905-910 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Keiichi Ishihara, Kunihiko Yasuda, and Takumi Hatayama: "Molecular cloning, expression and localization of human 105 kDa heat shock protein, hsp105" Biochim.Biophys.Acta. 1444(1). 138-142 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kunihiko Yasuda, keiichi Ishihara, Kazuo Nakashima, and Takumi Hatayama: "Genomic cloning and promoter analysis of mouse 105-kDa heat shock protein (hsp105) gene." Biochem.Biophys.Res.Commun.(in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takumi Hatayama: "Association of HSP105 with HSC70 in high molecular mass compexes in mouse FM3A cells." Biochem.Biophys.Res.Commun.248(2). 395-401 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tohru Wakatsuki: "Characteristic expression of 105-KDa heat shock protein (HSP105) in various tissues of nonstressed and heat-stressed rats." Biol.Pharm.Bull.21(9). 905-910 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Keiichi Ishihara: "Molocular cloning,expression and localization of hurnan 105 KDa heat shock protein,hsp105." Biochim.Biophys.Acta.1444(1). 138-142 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kunihiko Yasuda: "Genomic clening and promoter analysis of mouse 105-KDa heat shock protein (hsp105) gcen" Biochem.Biophys.Res.Commun.(印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takumi Hatayama: "Characteristic expression of high molecular mass heat shock protein HSP105 during mouse embryo development." Cell Struct.Funct.22. 517-525 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takumi Hatayama: "Reduced induction of HSP70 in PC12 cells during neuronal differentiation." J.Biochem.122. 904-910 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takumi Hatayama: "Effects of hyperosmotic Nacl and glycerol Stress on stress response of human HeLa cells." Biol.Pharm.Bull.20. 605-612 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hiroshi Oshima: "A possibility for new evaluating method of cytotoxicity by using heat shock protein assuy" J.Mater.Sci. : Mater.med.8. 143-147 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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