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Studies on integtin signaling in platelet adhesion and aggregation

Research Project

Project/Area Number 09670150
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionGIFU UNIVERSYTI

Principal Investigator

BANNO Yoshiko  SCHOOL OF MEDICINE,GIFU UNIVERSITY LECTURER, 医学部, 講師 (50116852)

Co-Investigator(Kenkyū-buntansha) NAKASHIMA Shigeru  SCHOOL OF MEDICINE,GIFU UNIVERSYTI ASSOCIATE PROFESSOR, 医学部, 助教授 (60188935)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsHuman platelet / Phospholipase C / Integrin signaling / Gbetagamma subunit / 血小板 / インテグリンGPIIb / IIIa / PI-PLC / チロシンリン酸化 / 接着
Research Abstract

It has been known that the platelet integrin alpha_<IIb>beta_3 is involved in platelet aggregation and adhesion. When platelets are stimulated by agonists, intracellular signal transduction can be activated, leading to a conformational change in the extracellular domains of integrin alpha_<IIb>beta_3 that increases its affinity for adhesive ligands, for example fibrinogen, resulting in platelet aggregation. Recent study has demonstrated that Galphaq is important in platelet aggregation induced by all agonists and Gi or G12/13 are involved in shape change. To investigate a role of phospholipase C (PLC) isozymes in the integrin alpha_<IIb>beta_3-mediated signaling, their location was examined in thrombin-activated human platelets, revealing different regulation of their translocation to the cytoskeleton (CSK). PLCbeta3b (l4OkDa) was present only in the cytosolic fraction and thrombin stimulation caused a rapid and transient translocation of PLCbeta3(a, b) in the reorganized CSK.The translocation to CSK of both PLCbeta3(a, b), but not PLCbeta2 was dependent on integrin alpha_<IIb>beta_3-mediated aggregation. The integrin alpha_<IIb>beta_3-mediated cytoskeletal association of PLCbeta3 was truncated by calpain. The truncated PLCbeta3 was activated G protein betagamma subunits. The human platelet Gi2 and Gq were consisted from beta1gamma5. No significant differences. for PLCbeta3 activation were not observed among various Gbetagamma subunits. These results suggested that combination of agonist-induced PLCbeta2/3 activation via Gqalpha and integrin-mediated PLCbeta3 activation via Gbetagamma may be important in platelet aggregation and adhesion.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (46 results)

All Other

All Publications (46 results)

  • [Publications] Ohguchi,K: "Increased activity of small GTP-binding protein-dependent phospho-lipase D during differentiation in human promyelocytic leukemic HL60 cells" J.Biol.Chem.272. 1990-1996 (1977)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Banno,Y.: "Nuclear ADP-ribosylation factor(ARF)-and oleate-dependent phospholipase D (PLD)in rat liver cells" J.Biol.Chem.272. 5208-5213 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ito,Y: "Implication of Ca^<2+>-dependent protein tyrosine phosphorylation in carbachol-induced phospholipase D activation in PC12 cells" J.Neurochem.68. 419-425 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takamura,O.S: "Inhibition of N-formylethionyl-leucyl-phenylalanine-stimulated tyrosine phosphorylation and phospholipase D activation by quercetin in rabbit neutrophils" Biochem.Pharmacol.53. 1503-1510 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshimura,Y.: "Changes in the activity and mRNA levels of phospholipase D during ceremide-induced apoptosis in rat glial cellsishimura" J.Neurochem.,. 69. 713-720 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hammond,S.M.: "Characterization of two alternately spliced forms of phospholipase D1" J.Biol.Chem.272. 3860-3868 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshimura,S.: "Ceramide formation leads to caspase-3 activation during hypoxic PC12" J.Biol.Chem.273. 6921-6927 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Iwasaki-Bessho,Y.: "Decreased phospholipase D(PLD)activity in ceramide-induced apoptosis of human keratinocyte cell line HaCaT" J.Invest.Dermatol.110. 376-382 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ojio,K.: "Role of Rho family GTP-binding proteins in IgE receptor-mediated pgospgolipase D activation in mast cells" Biomed.Res.19. 53-63 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Banno,Y.: "Stimulation by G protein βγ subnits of pgospgolipase β isoforms in human platelets" Thromb.Haemost.79. 1008-1013 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Banno,Y.: "Evidence for the presence of multiple forms of sphingosine kinase in human platelets" Biochem.J.335. 159-170 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hayakawa,K.: "Decreased phosphokipase D activities in ceramide-induced apoptosis of RBL-2H3 mast cells" Biomed.Res.19. 159-170 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakashima,S.: "Advances in Molecular and Cell Biology Series, Vol.17, The Platelet" JAI Press Inc., Greenwich, 15 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakashima,S.: "Handbook of Experimental Pharmacology, Vol.126," Spring-Verlag,Heidelberg, 16 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 中島 茂: "シリーズ分子生物学3, 高等動物の分子生物学" 朝倉書店, 7 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 坂野喜子: "生物薬学実験講座" 廣川書店 (印刷中), (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ohguchi, K.: "Enhancement of phospholipase D activity during differentiation in human promyelocytic leukemic HL60 cells : involvement of RhoA translocation to membrane" J.Biol.Chem.272. 1990-1996 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Banno, Y.: "Nuclear ADP-ribosylation factor (ARF) -and oleate-dependent phospholipase D (PLD) in rat liver cells" J.Biol.Chem.272. 5208-5213 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakashima, S: "Purification of factor from CSF in patient after SAH which induces the cytosolic free calcium elevation in vascular smooth muscle cells" Neurol.Res.19. 51-56 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshimura, Y.: "Changes in the activity and mRNA levels of phospholipase D during ceramide-induced apoptosis in rat glial cellsishimura" J.Neurochem.69. 713-720 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takemura, O.S.: "Inhibition of N-formylmethionyl-leucyl-phenylalanine-stimulated tyrosine phosphorylation and phospholipase D activation by quercetin in rabbit neutrophils" Biochem.Pharmacol. 53. 1503-1510 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kato, Y.: "Involvement of rho family proteins in prostaglandin F2alpha-induced phospholipase D activation in the osteoblast-like cell line MC3T3-E1" Prostaglandins. 54. 475-492 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Dohjima, T.: "Preferential down-regulation of phospholipase C-beta1 (PLC-beta1) during the early stage of differentiation in MC3T3-E1 osteoblast cell" Biomed.Res.18. 445-451 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshimura, S.: "Ceramide formation leads to caspase-3 activation during hypoxic PC12 cell death Inhibitory effects of Bcl-2 on ceramide formation and caspase-3 activation" J.Biol.Chem.273. 6921-6927 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Iwasaki-Bessho, Y.: "Decreased phospholipase D (PLD) activity in ceramide-induced apoptosis of human keratinocyte cell line HaCaT." J.Invest.Dermatol.110. 376-382 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ojio, K.: "Role of Rho family GTP-binding proteins in IgE receptor-mediated phospholipase D activation in mast cells" Biomed.Res.19. 53-63 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Banno, Y.: "Stimulation by G protein betagamma subunits of phospholipase beta isoforms in human platelets" Thromb.Haemost. 79. 1008-1013 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Banno, Y.: "Evidence for the presence of multiple forms of sphingosine kinase in human platelets" Biochem.J.335. 301-304 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hayakawa, K.: "Decreased phospholipase D activities in ceramide-induced apoptosis of RBL-2H3 mast cells" Biomed.Res.19. 159-170 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakashima, S.: "Advances in Molecular and Cell Biology Series, Vol.17, The Platelet, (Lapetina, E.G.ed.)" JAI Press Inc., Greenwich. 253-274 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshimura,S.: "Ceramide formation leads to caspase-3 activation during hypoxic PC12 cell death Inhibitory effects of Bcl-2 on ceramide formation and caspase-3 activation" J.Biol.Chem.273. 6921-6927 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Iwasaki-Bessho,Y.: "Decreased phospholipase D (PLD) activity in ceramide-induced apoptosis of human keratinocyte cell line HaCaT." J.Invest.Dermatol.110. 376-382 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ojio,K.: "Role of Rho family GTP-binding proteins in IgE receptor-mediated phospholipase D activation in mast cells." Biomed.Res.19. 53-63 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Banno,Y.: "Stimulation by G protein βγ subunits of phospholipase β isoforms in human platelets" Thromb.Haemost.79. 1008-1013 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Banno,Y.: "Evidence for the presence of multiple forms of sphingosine kinase in human platelets" Biochem.J.335. 301-304 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hayakawa,K.: "Decreased phospholipase D activities in ceramide-induced apoptosis of RBL-2H3 mast cells" Biomed.Res.19. 159-170 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 中島 茂: "シリーズ分子生物学3,高等動物の分子生物学" 朝倉書店, 7 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 坂野喜子: "生物薬学実験講座" 廣川書店(印刷中),

    • Related Report
      1998 Annual Research Report
  • [Publications] Ohguchi,K.: "Enhancement of phospholipase D activity during differentiation in HL60 cells:involvement of RhoA translocation to membranes" J.Biol.Chem.272. 1990-1996 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Banno,Y.: "Nuclear ADP-ribosylation factor-and oleate-dependent phospholipase D in rat liver cells:Increases of ARF-dependent PLD activity in regenerating liver cells" J.Biol.Chem. 272. 5208-5213 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshimura,S.: "Changes in the activity and mRNA levels of phospholipase D during ceramide-induced apoptosis in rat glial cells." J.Neurochem.69. 713-720 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshimura,S.: "Ceramide formation leads to caspase-3 activation during hypoxic PC12 cell death: Inhibitory effects of Bcl-2 on ceramide formation and caspase-3 activation" Prostaglandins. 54. 475-492 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Banno,Y.: "Stimulation by G protein βγ subunits of phospholipase β isoforms in human platelets" Thromb.Haemost.(in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Kato,Y.: "Involvement of rho family proteins in prostaglandin F2α-induced phospholipase D activation in the osteoblast-like cell line MC3T3-E1" J.Biol.Chem.(in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakashima,S.: "The Platelet,(Lapetina,E.G.ed.)" JAI Press Inc., 19 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakashima,S.: "Handbook of Experimental Pharmacology,Vol.126,Platelets and Their Factors 24" Spring-Verlag, 15 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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