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Establishment of Immortalized Neuronal Cells from Transgenic Mice and Its Application to Neuronal Diseases

Research Project

Project/Area Number 09670162
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionMeijo University

Principal Investigator

KANEDA Norio  Meijo University, Faculty of Pharmacy, Professor, 薬学部, 教授 (00144139)

Co-Investigator(Kenkyū-buntansha) HIKITA Kiyomi  Meijo University, Faculty of Pharmacy, Research Associate, 薬学部, 助手 (30257654)
YOKOYAMA Minesuke  Mitsubishi Kasei Institute of Life Sciences, Cheaf, 生殖工学開発室, 室長
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsTransgenic Mice / Substantia Nigra / Neurodegenerative Disease / トランスジェニックマウス
Research Abstract

Parkinson's disease is a neurodegenerative disease in which a selective impairment of nigro-striatal dopaminergic neurons is occurred. To understand the basic mechanism of the selective cell death, nigro-striatal dopaminergic cell line would be extreamly useful. Since the cell number of dopaminergic neurons in substantia nigra is relatively low, and neurons don't proliferate, we used transgenic technology to overcome these problems.
In 1997, we constructed a transgene which is composed of 2.5 kb promoter region of human tyrosine hydroxylase (TH) gene fused to a coding region of SV40T.Using this DNA fragment, we produced 6 lines of Tg mice, in which 2 lines died about 8 weeks after birth and their offsprings were not obtained. The remaining 4 lines were maintained.
In 1998, we tried to culture mesencephalic neurons from the embryos of Tg mice. Cells were cultured at a permissive 33C for 1 week, and analysed by immunocytochemistry. In a double staining experiment, several immuno-positive clones for SV40T were found, but were not immuno-positive for TH.To make the isolation of neuronal cells more easy, we are planning to produce Tg mice carrying a neomycine registant gene under the same promoter region of TN.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] N.KANEDA: "Identification of the Essential Crysteinyl Residue Located in the Active Site of Human Phenylethanolamine N-Methyltransferase" Biochem.Biophys.Res.Commun.249. 405-409 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.IMAISO: "A Novel Mutation of the GTP-Cyclohydrolase I Gene in a Patient with Hereditary Progressive Dystonia/Dopa-Responsive Dystonia" Neurology. 50. 517-519 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] N.Kaneda, K.Hikita, Y.Naruse, T.Fukuo, K.Matsubara and T.Nagatsu: "Identification of the Essential Cysteinyl Residue Located in the Active Site of Human Phenylethanolamine N-Methyltransferase" Biochem.Biophys.Res.Commun.249. 405-409 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Imaiso, T.Taniwaki.T.Yamada, T.Yoshimura, M.Hirano, S.Ueno, N.Kaneda and J.Kira: "A Novel Mutation of the GTP-Cyclohydrolase I Gene in a Patient with Hereditary Progressive Dystonia/Dopa-Responsive Dystonia" Neurology. 50. 517-519 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] N.KANEDA: "Identification of the Essential Cysteinyl Residue Located in the Active Site of Human Phenylethanolamine N-Methyltransferase" Biochem.Biophys.Res.Commun.249. 405-409 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.IMAISO: "A Novel Mutation of the GTP-Cyclohydrolase I Gene in a Patient with Hereditary Progressive Dystonia/Dopa-Responsive Dystonia" Neurology. 50. 517-519 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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