| Project/Area Number |
09670166
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Saitama Cancer Center |
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Principal Investigator |
KASIKABE Takashi Saitama Cancer center, Research Institute, Chief Researcher, 研究所, 主任研究員 (50152658)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | MmTRA1 gene / phospholipid scramblase / oncogene / transplantaility / monocytic leukemia cells / differentiation / apoptosis / 白血病細胞 / 増殖分化 / 細胞膜 / Mm TRA1遺伝子 |
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| Research Abstract |
We cloned a new leukemogenesis-associated gene MmTRA1a from a mouse leukemogenic and monocytic Mm-P cell cDNA library and also cloned its normal counterpart MmTRA1 from a normal mouse kidney cDNA library. The mouse MmTRA1a is a truncated form of mouse MmTRA1b. The mouse MmTRA1a mRNA was exclusively expressed in leukemogenic but not in nonleukemogenic Mm sublines and normal tissues. The MmTRA1b mRNA was expressed in normal tissues but not in any Mm sublines. After leukemogenic Mm-P cells were induced to differentiate into normal macrophages by sodium butyrate, the normal counterpart MmTRA1b was expressed, whereas the MmTRA1a level decreased. Transfection of MmTRA1a converted nonleukemogenic cells into leukemogenic cells. We also cloned a human homologue of mouse MmTRA1b cDNA from human monocytic U937 cDNA library. Expression of the human MmTRA1b was increased during differentiation inducers. The human MmTRA1b gene was mapped to chromosome 3q23. The predicted amino acid sequence analysis of human MmTRA1b cDNA revealed perfect identity with the human plasma membrane phospholipid scramblase which was recently reported its cDNA cloning. We also detected the expression of human MmTRA1a mRNA by RT-PCR in U937 cells.
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