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Functional Characterization of LIMK2 carrying LIM and PDZ Domains in the Developmental and Tumorigenic Process.

Research Project

Project/Area Number 09670170
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionAichi Cancer Center

Principal Investigator

OSADA Hirotaka  Aichi Cancer Center, Pathophysiology Unit, Section Head, 病態学研究室, 室長 (30204176)

Co-Investigator(Kenkyū-buntansha) YATABE Yasushi  Aichi Cancer Center, Research Institute, Researcher, 研究所, 研究員 (90280809)
MASUDA Akira  Aichi Cancer Center, Laboratory of Ultrastructure Research, Researcher, 超微形態学部, 主任研究員 (50157202)
TAKAHASHI Takashi  Aichi Cancer Center, Laboratory of Ultrastructure Research, Laboratory Head, 超微形態学部, 部長 (50231395)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsLung Cancer / Oncogene / がん遺伝子
Research Abstract

We previously isolated the human LIMK2 gene and identified two alternative transcripts, LIMK2a and LIMK2b, which were differentially regulated in a tissue-specific manner. To examine this differential tissue-specific expression in detail, an RNase protection assay was performed, which demonstrated three expression patterns of the LIMK2 isoforms. In digestive organs, the LIMK2a transcripts were preferentially expressed in fetal and adult tissues ; in brain and lung, the LIMK2a transcript was predominantly expressed only in fetal tissue, and in placenta, the LIMK2b transcript was expressed more abundantly than that of LJMK2a. To further investigate this mechanism and the transcription factors involved, we isolated the two distinct 5' upstream regions from the phage genomic library, and found that both LIMK2a and 2b promoters have a single major transcription initiation site and thecharacteristics of a TATA-less promoter. A luciferase reporter assay of the transcriptional activity revealed positive as well as negative regulatory regions within both promoters. The RORalphal may be involved in the positive regulation for the LIMK2b expression, while the MZF-1 might regulate both LIMK2a and 2b in a different manner. The genomic structure of the LIMK2 gene was also determined. These findings should lead to a better understanding of the possibly diverse functions of the LIMK family

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Yanagisawa, K., et al.: "Induction of apoptosis by Smad3 and down-regulation of Smad3 expression in respose to TGF-β in human normal lung epithelial cells." Oncogene. 17. 1743-47 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kondo, M., et al.: "Molecular cloning of human TAKl and its mutational analysis in human lung cancer." International Journal of Cancer. 75. 559-63 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takagi, Y., et al.: "p53 mutations in non-small-cell lung cancers occurring in individuals without a past history of active smoking." British Journal of Cancer. 77. 1568-72 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yatabe, Y., et al.: "p27KIPl in human lung cancers : differential changes in small cell and non-small cell carcinomas." Cancer Research. 58. 1042-47 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takahashi, T., et al.: "Molecular analysis of a Myc antagonist, ROX/Mnt, at 17p13.3 in human lung cancers." Japanese Journal of Cancer Research. 89. 347-51 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Osada, H.et al.: "The LIM-only protein Lmo2 is a bridging molecule assembling an erythroid, DNA-binding complex which includes the TAL1, E47, GATA-1 and Ldb1/NLI proteins." EMBO Journal. 16. 3142-57 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Osada, H.et al.: "LIM-only protein Lmo2 froms a aprotein complex with erythroid transcription factor GATA-1." Leukemia. 11S3. 307-12 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Rabbitts, T.H., et al.: "Chromosomal translocations and leukaemia : a role for LMO2 in T cell acute leukaemia, in transcription and in erythropoiesis." Leukemia. 11S3. 271-72 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Uchida, K., et al.: "Molecular cloning of CISH, chromosome assignment to 3p21.3, and analysis of expression in fetal and adult tissues." Cytogenetics & Cell Genetics. 78. 209-12 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Uchida, K., et al.: "Altered transcriptional regulation of the insulin-like growth factor 2 gene in human hepatocellular carcinoma." Molecular Carcinogenesis. 18. 193-98 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yanagisawa, K., et al.: "Induction of apoptosis by Smad3 and down-regulation of Smad3 expression in response to TGF-beta in human normal lung epithelial cells." Oncogene. 17. 1743-47 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kondo, M,, et al.: "Molecular cloning of human TAK1 and its mutational analysis in human lung cancer." Int.J.Cancer. 75. 559-63 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takagi, Y., et al.: "p53 mutations in non-small-cell lung cancers occurring in individuals without a past history of active smoking." Br.J.Cancer. 77. 1568-72 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takahashi, T., et al.: "Molecular analysis of a Myc antagonist, ROX/Mnt, at 17p13.3 in human lung cancers." Jpn.J.Cancer Res. 89. 347-51 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yatabe, Y., et al.: "p27KIP1 in human lung cancers : differential changes in small cell and non-small cell carcinomas." Cancer Res.58. 1042-7 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Osada, H.et al.: "The LIM-only protein Lmo2 is a bridging molecule assembling an erythroid, DNA-binding complex which includes the TAL1, E47, GATA-1 and Ldb1/NLI proteins." EMBO J.16. 3145-57 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Osada, H., et al.: "LIM-only protein Lmo2 forms a protein complex with erythroid transcription factor GATA-1" Leukemia. 11 Suppl 3. 307-12 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Rabbitts, T.H., et al.: "Chromosomal translocations and leukaemia : a role for LMO2 in T cell acute leukaemia, in transcription and in erythropoiesis." Leukemia. 11 Suppl 3. 271-2 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Uchida, K., et al.: "Molecular cloning of CISH,chromosome assignment to 3p21.3, and analysis of expression in fetal and adult tissues." Cytogenet.Cell Genet.78. 209-12 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Uchida, K., et al.: "Altered transcriptional regulation of the insulin-like growth factor 2 gene in human hepatocellular carcinoma." Mol Carcinog.18. 193-8 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yanagisawa,K.,et al.: "Induction of apoptosis by Smad3 and down-regulation of Smad3 expression in response to TGF-β in human normal lung epithelial cells." Oncogene. 17. 1743-1747 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kondo,M.,et al.: "Molecular cloning of human TAKI and its mutational analysis in human lung cancer." International Journal of Cancer. 75. 559-563 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takagi,Y.,et al.: "p53 mutations in non-small-cell lung cancers occurring in individuals without a past history of active smoking." British Journal of Cancer. 77. 1568-1572 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yatabe,y.,et al.: "p27KIP1 in human lung cancers:differential changes in small cell and non-small cell carcinomas." Cancer Research. 58. 1042-1047 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takahashi,T.,et al.: "Molecular analysis of a Myc antagonist,ROX/Mnt,at 17p13.3 in human lung cancers." Japanese Journal of Cancer Research. 89. 347-351 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Osada H, Wadman IA, et al.: "The LIM-only protein Lmo2 is a bridging molecule assembling an erythroid,DNA-binding complex which includes the TAL1,E47,GATA-1 and Ldb/NLI proteins." EMBO Journal. 16. 3145-3157 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Osada H, Grutz GG,e t al.: "LIM-only protein Lmo2 forms a protein complex with erythroid transcription factor GATA-1" Leukemia. 11(S3). 307-312 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Uchida K, Yoshimura,A, et al.: "Molecular cloning of CISH,chromosome assignment to 3p21.3,and analysis of expression in fetal and adult tissues." Cytogenetics & Cell Genetics. 78. 3-4 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kondo M, Osada H, et al.: "Molecular cloning of human TAK1 and its mutational analysis in human lung cancer" International Journal of Cancer. 75. 559-563 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yatabe Y, Masuda A, et al.: "p27KIP1 in human lung cancers: differential changes in small cell and non-small cell carcinomas" Cancer Research. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takagi Y, Osada H, et al.: "p53 mutations in non-small cell lung cancers occurring in individuals without a past history of active smoking." British Journal of Cancer. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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