| Project/Area Number |
09670177
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Human pathology
|
|---|
| Research Institution | SCHOOL OF MEDICINE, CHIBA UNIVERSITY |
|---|
Principal Investigator |
ISHII Genichiro (1998) CHIBA UNIVERSITY,SCHOOL OF MEDICINE.Assistant, 医学部, 助手 (00270869)
三方 淳男 (1997) 千葉大学, 医学部, 教授 (40051289)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HARIGAYA Kenichi CHIBA UNIVERSITY,SCHOOL OF MESICINE.Proffesor, 医学部, 教授 (40101894)
TAMARU Junichi CHIBA UNIVERSITY,SCHOOL OF MESICINE.Lecturer, 医学部, 講師 (30188429)
石井 源一郎 千葉大学, 医学部, 助手 (00270869)
|
|---|
| Project Period (FY) |
1997 – 1998
|
| Project Status |
Completed (Fiscal Year 1998)
|
| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
|
|---|
| Keywords | Epstein-Barr virus / Latent membrane protein-1 / Hodgkin's disease / Gastric carcinoma / EBウイルス / LMP-1 / ホジキン病 / 胃癌 / 欠失LMP-1遺伝子 / EBV種地域差 / EBV関連胃癌 / bcl-2免疫染色 |
|---|
| Research Abstract |
The presence of 30-bp deletions within the carboxy terminal end of the LMP-1 oncogene of EBV has been reported in EBV-aasociated neolasms. We analysed the 30-bp deletion of the LMP-1 gene in Hodgkin's disease and gastric carcinoma in Japan and Nepal. 35% of Hodgkin's cells in Japan showed positive reaction for LMP-1. Direct sequence analysis of the PCR products of carboxy terminal end of the LMP-1 oncogene revealed identical 30-bp deletion in 5 of 6 samples of EBV-associated Hodgkin's disease. In EBV-associated gastric carcinoma in Japan, this deletion was found in 2 of 3 samples.
On the other hand, 100% of Hodgkin's cells showed positive reaction for LMP-1 in Nepal. Identical 30-bp deletion was observed in 4 of 8 samples.
Furthermore we examined the proliferating activity and P53 expression of Hodgkin's cells with and without 30-bp deletion. However proliferating activity and P53 expression was almost similar between these two groups.
Our results suggest that the 30-bp deletion mutant is widely spread in Japan and Nepal and is not implicated in EBV-associated neolasms.
|
