| Project/Area Number |
09670181
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Gifu University School of Medicine |
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Principal Investigator |
TAKAMI Tsuyoshi Gifu University School of Medicine, Professor, 医学部, 教授 (70136943)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAYAMA Takashi Gifu University School of Medicine, Assistant, 医学部, 助手 (20293558)
SAIO Masanao Gifu University School of Medicine, Assistant, 医学部, 助手 (40242721)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Hepatoma / HLA-A24 / genetic engineering / Hpe.3B2.1-7 / Acid elution / FPLC / Peptide / 肝癌細胞 / 腫瘍抗原 / Hep-3B細胞 / 抗HLA-A24抗体 / ペプチド・シークエンス / 肝癌腫瘍抗原 / Hep3B / cDNA / 単クローン抗体 |
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| Research Abstract |
Human hepatoma cell line, Hep.3B2.1-7 that has no HLA-A24 gene expression, has been transduced of HLA-A24 cDNA by using mammalian cell line expression vector pcDNA3 .1 in order to carry the endogenous antigenic peptides on its cell surface. The acidic eluate of cloned HLA-A24 expressing Hep.3B2.1-7 (Hep.3B.A24) demonstrated seven significant peakes that were different from wild type Hep.3B2.1-7 and moke-transfected Hep.3B2.1-7 after FPLO fractionations.
One out of seven peptide fraction had anchoring motief for HIA-A24 at second (L) and tenth (K) positions, while two histon H2A, one HLA-A68 binding peptide of macrophage migration inhibiting factor, one HIA-B35 binding peptide, and two unknown peptides were clarified from remaining six fractions.
The homology research of HLA-A24 binding peptide obtained in present study demonstrated no homologous protein in data base.
In conclusion, present study is possible to clarify a novel HLA-A24 binding peptide, and HLA.molecules enforced to express by genetic engineering method will open a new avenue for the research in tumor-antigenic peptides.
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