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Molecular pathological study on the expression of apoptosis-related genes and regulating factors in human gastrointestinal carcinomas :

Research Project

Project/Area Number 09670185
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionTottori University

Principal Investigator

ITO Hisao  Tottori University, Faculty Medicine, Pathology, Professor, 医学部, 教授 (60127610)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsGastrointestinal carcinoma / apoptosis / p53 gene / cell cycle / dThdPase / Anticancer agent / Hyperthermia / Fas / エタノール / 熱ショック / FAS
Research Abstract

1) Adenovirus-mediated transfer of wild type p53 gene of 50 MCI into the human gastric and colonic carcinoma cell lines resulted in apoptotic cell death , growth arrest, or non-effectiveness. After infection with AxCA-p53, the expression levels of bax or bcl-XL protein changed in the cell lines showing apoptosis.
2) Human gastric and colonic carcinoma cell lines showed Fas expression variably. Anti-Fas antibody induced apoptosis of the gastric cell lines, regardless of p53 gene status, while the antibody induced apoptosis only in LoVo carrying wild type p53 gene of the colonic cell lines. AxCA-p53 transfection resulted in up-regulation of Fas.
3) Hyperthermia for 30 min. resulted in apoptosis in human gastric carcinoma cell lines, while necrosis was induced by the treatment for 60 min. The former showed up-regulation of P53.
4) The progression of gastric carcinoma was defined by a gradual increase of proliferative activity and constant occurrence of apoptosis and naturally occurring apoptosis is induced predominantly via a p53 gene-independent pathway.
5) Preoperative treatment with tegafur suppository significantlyenhanced apoptotic cell death and abated intratumoral microvessel density (IMVD) in human colorectal carcinomas.
6) IMVD was significantly higher in gastric and colonic carcinomas with dThdPase expression than in those without the expression, regardless the tumor stage. dThdPase expression was correlated with decrease of apoptos is.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Shigeru Tatebe, Hisao Ito, et al: "Taxol induces apoptosis in human colon carcinoma cell lines arrested in G_2/M phase" Oncology Reports. 4. 1151-1156 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hisae Hayashi, Hisao Ito, et al: "Expression of Fas antigen and its mediation of apoptosis in human gastric cancer cell lines" Jpn.J.Cancer Res. 88. 49-55 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mitsuyuki Ikeda, Hisao Ito, et al: "Frequent occurrence of apoptosis is an early event in the oncogenesis of human gastric carcinoma" Virchows.Arch. 432. 43-47 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shigeru Tatebe, Hisao Ito, et al: "Adenoviral transduction efficiency partly cirrelates with expression levels of integrin αvβ5, but not αvβ3 in human gastric carcinoma cells" Int.J.Mol.Med. 2. 61-64 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Satoshi Ofuji, Hisao Ito, et al: "Expression of MAGE-1, MAGE-2 and MAGE-3 genesin human gastric carcinomas ; lack of evidence for cytotoxic effects in cases with simultaneous expression of MAGE-3 and HLA-A2" Anticancer Res. 18(5B). 3639-3644 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Akira Goto, Hisao Ito, et al: "Hyperthermia-induced apoptosis occus both in a p53 gene-dependent manner in three human gastric carcinoma cell lines" Oncology Reports. 6. 335-339 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hisao Ito, et al: "Stomach cancer and apoptosis : A review" Molecular Pathology of Gastroenterological Cancer Spriger-Verlag, 11 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shunichi Tsujitani, Hisao Ito, et al: "Apoptotic cell death in relation to development of tumors of the stomach" Progress in Gastric Cancer Research, 5 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shigeru Tatebe, Hisao Ito, et al: "Taxol induces apoptosis in human colon carcinoma cell lines arrested in G2/M phase" Oncology Reports. 4. 1151-1156 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hisae Hayashi, Hisao Ito, et al: "Expression of Fas antigen and its mediation of apoptosis in humangastric cancer cell lines" Jpn.J.Cancer Res. 88. 49-55 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mitsuyuki Ikeda, Hisao Ito, et al: "Frequent occurrence of apoptosis is an early event in theoncogenesis of human gastric carcinoma" Virchows Arch. 432. 43-47 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shigeru Tatebe, Hisao Ito, et al: "Adenoviral transduction efficiency partly cirrelates with expression levels of integrin alphavbeta5, but not alphavbeta3 in human gastric carcinoma cells" Int.J.Mol.Med. 2. 61-64 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Satoshi Ofuji, Hisao Ito, et al: "Expression of MAGE-1, MAGE-2 and MAGE-3 genes in human gastriccarcinomas ; lack of evidence for cytotoxic effects in cases with simultaneous expression of MAGE-3 and HLA-A2" Anticancer Res. 18 (5B). 3639-3644 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Akira Goto, Hisao Ito, et al: "Hyperthermia-induced apoptosis occurs both in a p53 gene-dependent manner in three human gastric carcinoma cell lines" Oncology Reports. 6. 335-339 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hisao Ito, et al: "Stomach cancer and apoptosis : A review" Molecular Pathology of Gastroenterological Cancer. Spriger-Verlag 11. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shunichi Tsujitani, Hisao Ito, et al: "Apoptotic cell death in relation to development of tumors of the stomach" Progress in Gastric Cancer Research. 5. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Hirai: "Purification and characterization of trypsin inhibitor produced by plerocercoids of Spirometra eriraceieuropaei." 9^<th> International Congress of Parasitology. 1153-1158 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Wang: "Molecular cloning and expression of the gene encording a growth factor of Spirometra erinaceieuropaei." 9^<th> Interantional Congress of Parasitology. 831-834 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Fukumoto: "Excretory/secretory products from Plerocercoids of Spirometra erinaceieuropaei reduce iNOS and cnemokine mRNA levels in murine macrophages‥‥" 9^<th> Internatinal Congress of Parasitology. 545-550 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Miura: "Plerocercoids of Spirometra erinaceieuropaei reduce gene expression of tumor necrosis factor-α in mice." 9^<th> International Congress of Parasitology. 1241-1245 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mitsuhiko Osaki: "5-Fluororacil(5-FU)induced apoptosis in gastric cancer cell lines : role of the p53 gene" Apoptosis. 2・2. 221-226 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shigeru Tatebe: "Taxol induces apoptosis in human colon carcinoma cell lines arrested in G2/M phase" Oncology Reports. 4. 1151-1156 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hisae Hayashi: "Expression of Fas antigen and its mediation of apoptosis in human gastric cancer cell lines" Jpn J Cancer Res. 88. 49-55 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masato Ishida: "Evidence that expression of a mutated p53 gene attenuates apoptotic cell death in human gastric intestinal-type carcinomas in vivo" Jpn J Cancer Res. 88. 468-475 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hisao ito: "Molecular Pathology of Gastroenterological Cancer" Stomach cancer and apoptosis : A review, 11 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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