• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

CHROMOSOMAL AND GENETIC ABERRATIONS RELATED TO THE DEVELOPMENT AND PROGRESSION OF ESOPHAGEAL CANCER

Research Project

Project/Area Number 09670187
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionYAMAGUCHI UNIVERSITY

Principal Investigator

SASAKI Kohsuke  YAMAGUCHI UNIVERSITY, SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (80116722)

Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsesophagus / cancer / DNA / molecular cytogenetics / CGH / metastasis / proliferation / carcinogenesis / Cancer / Esophagus / Chromosome / Genoma / Gene / FISH / FCM / Cytometry / LSC / 食道癌 / 染色体 / 遺伝子
Research Abstract

(1) Surgical specimens.
In this project, 34 esophageal squamous cell carcinomas were analyzed by comparative benomic hybridization (CGH). DNA copy number increases were found at 3q (75%), 8q23-qter (50%), 11q13 (44%), 5p14-pter (25%), 20q (25%), 7q (22%), and 2p (19%), and decreases at 18q (58%), 3p (50%), 9p (44%), 5q14-23 (39%), 4q (33%), 13q (22%), and 11q22-qter (19%). DNA amplification (high-level gain) was detected at 11q13, 2q12, 7q21, 20q11.2. The comparison of these genetic changes with clinicopathological findings revealed that lymph node metastasis of cancer was closely associated with gains of 8q23-qter (p<0.0005) and 20q (p<0.02), and loss of 11q22 (p<0.05). These results suggest that diagnosis of lymph node metastasis is estimated in bioptic specimens. Since gains of 3q and 11q13, and losses of 18q, 3p, 9p, 5q14-23 and 4q were observed in both early and advanced cancers, these are considered to be genetic alterations involved in carcinogenesis of the esophagus.
(2) Cell lines.
We analyzed 6 cell lines established from human esophageal squamous cell carcinomas by CGH.Gains of 3q, 5p, 7p, 8q, 11q, 13q, 17p, 20q, and Xq, and losses of 4q, 9q, and 18q were common to these 6 cell lines. Gains of 7p12-13, 11q14-22, and 11q22-qter, and losses of 4p, 8p, and 11p14-qter were found exclusively in 3 cell lines which proliferale in medium without fetal serum, whereas gains of 12p and 20p, and losses of 3p and 5q were seen only in others which need fetal serum to grow. These findings suggest that characteristics of cell proliferation are related to genetic changes of tumor cells.
Genetic changes seen in cell lines were compatible with those in surgical specimens.

Report

(5 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (41 results)

All Other

All Publications (41 results)

  • [Publications] Tada K.: "Gains of 8q23-qter and 20q and loss of IIq22-qter in esophageal squamous cell carcinoma associated with lymph node metastasis."Cancer. 88. 268-273 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tada K.: "Cytogenetic analysis of esophageal squamous cell carcinoma cell lines by comparative genomic hybridization : Relationship of cytogenetic aberrations to in vitro cell growth."Cancer Genetic & Cytogenetics. 117. 108-112 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Okafuji M.: "The relationship of genetic aberrations detected by comparative genomic hybridization to DNA ploidy and tumor size in human oral squamous cell carcinomas."J Oral Pathol Med. 29. 226-231 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suehiro Y.: "Genetic aberrations detected by comparative genomic hybridization predict outcome in patients with endometrial carcinoma."Genes Chromosomes Cancer. 29. 75-82 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hashimoto Y.: "Relationship between cytogenetic aberrations by CGH coupled with tissue microdissection and DNA ploidy by laser scanning cytometry in head and neck squzamous cell carcinoma."Cytometry. 40. 161-166 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sato T.: "Cytogenetic aberrations detected by flow cytometry and fluorescence in situ hybridization in colorectal cancers : Two cytogenetic pathways in colorectal carcinogenesis."Oncology. 63. 63-69 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Okafuji M.: "Identification of genetic aberrations in cell lines from oral squamous cell carcinomas by comparative genomic hybridization."J Oral Pathol Med. 28. 241-245 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kusano N.: "Genetic aberrations detected by comparative genomic hybridization in hepato-cellular carcinomas : Their relationship to clinicopathological features."Hepatology. 117. 1858-1862 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shiraishi K.: "Genetic aberrations detected by comparative genomic hybridization in biliary tract cancers."Oncology. 57. 42-49 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Miyazaki M.: "The relationship of DNA ploidy to chromosomal instability in primary human colorectal cancers."Cancer Research. 59. 5283-5285 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Harada K.: "Intratumoral cytogenetic heterogeneity detected by comparative genomic hybridization and laser scanning cytometry in human gliomas"Cancer Research. 58. 4694-4700 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 佐々木功典: "応用サイトメトリー"医学書院. 369 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tada K.: "Gains of 8q23-qter and 2Oq and loss of 11q22-qter in esophageal squamous cell carcinoma associated with lymph node metastasis."Cancer. 88. 268-273 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tada K.: "Cytogenetic analysis of esophageal squamous cell carcinoma cell lines by comparative genomic hybridization : Relationship of cytogenetic aberrations to in vitro cell growth."Cancer Genetics & Cytogenetics. 117. 108-112 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Okafuji M.: "The relationship of genetic aberrations detected by comparative genomic hybridization to DNA ploidy and tumor size in human oral squamous cell carcinomas."J Oral Pathol Med. 29. 226-231 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suehiro Y.: "Genetic aberrations detected by comparative genomic hybridization predict outcome in patients with endometrial carcinoma."Genes Chromosomes Cancer. 29. 75-82 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hashimoto Y.: "Relationship between cytogenetic aberrations by CGH coupled with tissue microdissection and DNA ploidy by laser scanning cytometry in head and neck squamous cell carcinoma."Cytometry. 40. 161-166 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sato T.: "Cytogenetic aberrations detected by flow cytometry and fluorescence in situ hybridization in colorectal cancers : Two cytogenetic pathways in colorectal carcinogenesis."Oncology. 63. 63-69 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Okafuji M.: "Identification of genetic aberrations in cell lines from oral squamous cell carcinomas by comparative genomic hybridization."J Oral Pathol Med. 28. 241-245 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kusano N.: "Genetic aberrations detected by comparative genomic hybridization in hepatocellular carcinomas : Their relationship to clinicopathological features."Hepatology. 117. 1858-1862 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shiraishi K.: "Genetic aberrations detected by comparative genomic hybridization in biliary tract cancers."Oncology. 57. 42-49 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Miyazaki M.: "The relationship of DNA ploidy to chromosomal instability in primary human colorectal cancers."Cancer Research. 59. 5283-5285 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Harada K.: "Intratumoral cytogenetic heterogeneity detected by comparative genomic hybridization and laser scanning cytometry in human gliomas"Cancer Research. 58. 4694-4700 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sasaki K.: Igakusyoinn.Applied Cytometory. 369 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tada K.: "Gains of 8q23-qter and 20q and loss of 11q22-qter in esophageal squamous cell carcinoma associated with lymph node metastasis."Cancer. 88. 268-273 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tada K.: "Cytogenetic analysis of esophageal squamous cell carcinoma cell lines by comparative genomic hybridization : Relationship of cytogenetic aberrations to in vitro cell growth."Cancer Genetic & Cytogenetics. 117. 108-112 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Okafuji M.: "The relationship of genetic aberrations detected by comparative genomic hybridization to DNA ploidy and tumor size in human oral squamous cell carcinomas."J Oral Pathol Med. 29. 226-231 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hashimoto Y.: "Relationship between cytogenetic aberrations by CGH coupled with tissue microdissection and DNA ploidy by laser scanning cytometry in head and neck squamous cell carcinoma."Cytometry. 40. 161-166 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 佐々木功典: "応用サイトメトリー"医学書院. 369 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Miyazaki M,: "The relationship of DNA ploidy to chromosomal instability in primary human colorectal cancer"Cancer Research. 59. 5283-5285 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ozaki S,: "Compartive genomic hybridization analysis of genetic・・"Neuro-Onocology. 41. 167-174 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Okajuji M,: "Identification of genetic aberrations in cell lines…"Journal of Oral Pathology. 28. 241-245 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kusano N,: "Genetic aberrations detected by comparative genomic…"Hepatology. 29. 1858-1862 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shiraishi K,: "Genetic aberrations detected by comparative genomic…"Oncology. 57. 42-49 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toshihiko Sato: "Cytognetic aberrations detected by FCM and FISH in colorectal cancers" Oncology. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Masaki Okafuji: "Idenfitifaction of genetic aberrations by comparative genomic hybridization in oral squamous cell carcinomas" J Oral Pathology and Medicine. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Katsumi Harada: "Intratumoral cytogenetic heterogeneity detected by comparative genomic hybridization and laser....." Cancer Research. 58. 4694-4700 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takafumi Nishizaki: "Investigation of genetic alterations asscoiated with the grade of astrocytic tumor by comparative genomic.." Genes Chromosomes Cancer. 21. 340-346 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Satoshi Ozaki: "Comparative genomic hybridization analysis of genetic alterations associated with malignant progression..." J Neuro-Oncol. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] 佐々木 功典: "FISHを利用した大腸腺腫と腺癌の診断" 病理と臨床. 16(1). 44-49 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 佐々木 功典: "遺伝子の発現を解析:In situ hybridizationクロモソーマルISH" 肝胆膵. 35(6). 743-747 (1997)

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi